HLA-G Enhances the Killing Effect of NK Cells on Cervical Cancer Cells by Promoting the Expression of Activated Receptor

Qian LIU; Tuerxun HATILA; Hasimu AXIANGU

doi:10.12259/j.issn.2095-610X.S20220714

Volume 43 Issue 7

Jul. 2022

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Qian LIU, Tuerxun HATILA, Hasimu AXIANGU. HLA-G Enhances the Killing Effect of NK Cells on Cervical Cancer Cells by Promoting the Expression of Activated Receptor[J]. Journal of Kunming Medical University, 2022, 43(7): 46-54. doi: 10.12259/j.issn.2095-610X.S20220714

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Qian LIU, Tuerxun HATILA, Hasimu AXIANGU. HLA-G Enhances the Killing Effect of NK Cells on Cervical Cancer Cells by Promoting the Expression of Activated Receptor[J]. Journal of Kunming Medical University, 2022, 43(7): 46-54. doi: 10.12259/j.issn.2095-610X.S20220714

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HLA-G Enhances the Killing Effect of NK Cells on Cervical Cancer Cells by Promoting the Expression of Activated Receptor

doi: 10.12259/j.issn.2095-610X.S20220714

Xinjiang Key Laboratory of Molecular Biology of Endemic Diseases， School of Basic Medicine，Xinjiang Medical University，Urumqi Xinjiang 830054，China

Received Date: 2022-04-13
Available Online: 2022-06-25
Publish Date: 2022-07-14

Abstract

Abstract

Objective To explore the effect of HLA-G expression on NK cell surface activation receptor in cervical cancer cells. Methods Real-time PCR （RT-PCR） and Western Blot （WB） methods were used to examine the expression of HLA-G in cervical cancer cells and normal cervical cells, respectively. The cervical cancer cell lines transfected with lentivirus to silence the expression of HLA-G were co-cultured with NK cells. The proliferation ability of co-cultured cervical cancer cells was measured by the method of CCK8, and the level of apoptosis in cervical cancer cells was measured by the flow cytometry method, as well as the expression of NKG2D and NKP30, Granzyme and Perforin. Results The results of RT-PCR and WB showed that compared with H8 cells, the expressions of HLA-G in C33a and SiHa cells were significantly increased （P < 0.05）. Results of CCK8 showed that the proliferation of C33a and SiHa cells was significantly decreased （P < 0.05） when C33a and SiHa cells were co-cultured with NK cells after transfection. The levels of apoptosis in C33a and SiHa cells were significantly increased （P < 0.05）.The results of Flow cytometry showed that the expression of NKG2D on NK surface was significantly increased after C33a and SiHa were transfected （P < 0.05）. Of C33a, The expression of NKP30 on the surface of NK cells and Granzyme, Perforin were significantly increased （P < 0.05）. However, of C33a, the expression of NKP30 on the surface of NK cells and Granzyme, Perforin were increased, but the difference was not statistically significant （P > 0.05）. Conclusion The high expression of HLA-G is in cervical cancer cells, and it may enhance the killing effect of NK cells on cervical cancer cells by promoting the expression of activated receptor.
- Cervical cancer,
- HLA-G,
- NK cell,
- Immune escape

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References(23)

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