Scutellarin Ameliorates Liver Fibrosis in Nonalcoholic Fatty Liver Disease by Inhibiting NOX Expression

Yongrui YANG; Liyuan WANG; Haiwen LI; Zhirong ZHAO; Rui PU; Guishuai WU; Shude LI

doi:10.12259/j.issn.2095-610X.S20220721

Volume 43 Issue 7

Jul. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(7): 38-45

Yongrui YANG, Liyuan WANG, Haiwen LI, Zhirong ZHAO, Rui PU, Guishuai WU, Shude LI. Scutellarin Ameliorates Liver Fibrosis in Nonalcoholic Fatty Liver Disease by Inhibiting NOX Expression[J]. Journal of Kunming Medical University, 2022, 43(7): 38-45. doi: 10.12259/j.issn.2095-610X.S20220721

Citation:

Yongrui YANG, Liyuan WANG, Haiwen LI, Zhirong ZHAO, Rui PU, Guishuai WU, Shude LI. Scutellarin Ameliorates Liver Fibrosis in Nonalcoholic Fatty Liver Disease by Inhibiting NOX Expression[J]. Journal of Kunming Medical University, 2022, 43(7): 38-45. doi: 10.12259/j.issn.2095-610X.S20220721

Citation:

Yongrui YANG, Liyuan WANG, Haiwen LI, Zhirong ZHAO, Rui PU, Guishuai WU, Shude LI. Scutellarin Ameliorates Liver Fibrosis in Nonalcoholic Fatty Liver Disease by Inhibiting NOX Expression[J]. Journal of Kunming Medical University, 2022, 43(7): 38-45. doi: 10.12259/j.issn.2095-610X.S20220721

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Scutellarin Ameliorates Liver Fibrosis in Nonalcoholic Fatty Liver Disease by Inhibiting NOX Expression

doi: 10.12259/j.issn.2095-610X.S20220721

1.
Dept. of Hepatology，The Third Hospital of Kunming，Yunnan Provincial Center for Infectious Diseases Clinical Medicine，Kunming Yunnan 650041
2.
Dept. of Biochemistry and Molecular Biology，School of Basic Medicine
3.
School of Basic Medicine，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2022-04-05
Publish Date: 2022-07-25

Abstract

Abstract

Objective To investigate the effect of scutellarin （SCU） on the expression of NOX （NOX2, NOX4） and the degree of liver fibrosis induced by high fat and high sugar in non-alcoholic fatty liver disease （NAFLD）. Methods Sixty rats were randomly divided into normal group, model group, SCU-25 lower dose treatment group［25 mg/（kg·d）, SCU-50 dose in the treatment group［50 mg/（kg·d）］, SCU-100 high dose treatment group ［100 mg/（kg·d） and curcumin positive drug treatment group［200 mg/（kg·d）］, 10 in each group. The normal group was fed with ordinary diet for 24+8 weeks, and the other groups were fed with high-fat and high- sugar diet for 24 weeks to establish NAFLD animal model, and then given Different concentrations of SCU and curcumin intragastric treatment for 8 weeks. After the treatment, the weight of the rats was measured, and the serum samples and liver tissues of the rats were collected under anesthesia. The weight of theliver was measured and the liver index was calculated. ROS content in serum and tissues was detected by ELISA. Using oil red "O" Dyeing, detection of liver lipid deposition and Masson staining to detect the degree of fibrosis of liver tissue; Western blot and immunohistochemistry were used to detect the expression of NOX2 and NOX4 proteins in liver tissues. Results Body weight, liver weight and liver index increased in model group （ P < 0.01）, body weight, liver weight and liver index decreased after different doses of SCU and curcumin treatment group, while body weight and liver weight were significantly decreased in SCU-100 high-dose treatment group and curcumin positive drug treatment group （ P < 0.01）. ROS content in serum and liver tissues of model group was significantly increased （ P < 0.01）, and ROS content was decreased after treatment with different doses of SCU and curcumin （ P < 0.05）. In the liver tissue, the model group showed a large amount of lipid deposition and tissue fibrosis. After treatment with different doses of SCU and curcumin, the lipid deposition was reduced and the degree of liver fibrosis wasimproved. In liver tissue cells, the protein expressions of NOX2 and NOX4 were up-regulated in the model group （ P < 0.05）, but down-regulated in differentdoses of SCU and curcumin （ P < 0.05）, but not dose-dependent. Conclusion SCU may reduce ROS production by inhibiting the expression of NOX2 and NOX4 proteins in liver, thus alleviating the degree of liver fibrosis in NAFLD.
- Non-alcoholic fatty liver disease,
- Liver fibrosis,
- Scutellarin,
- Reactive oxygen species,
- NADPH oxidase

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