YLR358C Regulates Cell Wall Integrity of <i>Saccharomyces cerevisiae</i>

Yu ZHANG; Mengyan LI; Hanying WANG; Yanfang GUO; Jiaqing MA; Weiqun WANG

doi:10.12259/j.issn.2095-610X.S20220730

Volume 43 Issue 7

Jul. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(7): 33-37

Yu ZHANG, Mengyan LI, Hanying WANG, Yanfang GUO, Jiaqing MA, Weiqun WANG. YLR358C Regulates Cell Wall Integrity of Saccharomyces cerevisiae[J]. Journal of Kunming Medical University, 2022, 43(7): 33-37. doi: 10.12259/j.issn.2095-610X.S20220730

Citation:

Yu ZHANG, Mengyan LI, Hanying WANG, Yanfang GUO, Jiaqing MA, Weiqun WANG. YLR358C Regulates Cell Wall Integrity of Saccharomyces cerevisiae[J]. Journal of Kunming Medical University, 2022, 43(7): 33-37. doi: 10.12259/j.issn.2095-610X.S20220730

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YLR358C Regulates Cell Wall Integrity of Saccharomyces cerevisiae

doi: 10.12259/j.issn.2095-610X.S20220730

1.
Faculty of Basic Medical Science
2.
Science and Technology Achievement Incubation Center，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2022-04-11
Available Online: 2022-06-16
Publish Date: 2022-07-14

Abstract

Abstract

Objective To observe the effect of Saccharomyces cerevisiae （S. cerevisiae） YLR358C deletion on cell wall integrity. Methods The growth of YLR358CΔ yeast was observed by spot assay; the effect of YLR358C on yeast cell wall was analyzed by Calcofluor White （CFW） staining and transmission electron microscopy （TEM）; the signal pathway regulated by YLR358C was detected by transcriptome analysis and qRT-PCR. Results The growth of S. cerevisiae knockout with YLR358C was significantly inhibited on medium containing CFW, congored （CR） and sodium dodecyl sulfate （SDS） at different concentrations. The cell wall chitin was down-regulated by CFW staining and the cell wall thickness was reduced by TEM. Transcriptome sequencing and analysis indicated that YLR358C gene may be involved in the regulation of CWI signaling pathway. qRT-PCR showed that WSC3, SWI4 and HSP12 were differentially expressed after YLR358C knockout （P < 0.05）. Conclusion YLR358C may regulate the integrity of yeast cell wall, and has certain application potential in fermentation.
- Saccharomyces cerevisiae,
- Cell wall integrity,
- YLR358C,
- Transcriptome analysis

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References(11)

References

[1]	Kadam A A,Kamatkar J D,Khandare R V,et al. Solid-state fermentation:tool for bioremediation of adsorbed textile dyestuff on distillery industry waste-yeast biomass using isolated Bacillus cereus strain EBT1[J]. Environmental Science and Pollution Research International,2013,20(2):1009-1020. doi: 10.1007/s11356-012-0929-6
[2]	Sostaric N,Arslan A,Carvalho B,et al. Integrated multi-omics analysis of mechanisms underlying yeast ethanol tolerance[J]. Journal of Proteome Research,2021,20(8):3840-3852. doi: 10.1021/acs.jproteome.1c00139
[3]	Omura F,Takagi M,Kodama Y. Compromised chitin synthesis in lager yeast affects its Congo red resistance and release of mannoproteins from the cells[J]. FEMS Microbiology Letters,2020,367(22):fnaa181. doi: 10.1093/femsle/fnaa181
[4]	Sanz A B,Garcia R,Rodriguez-Pena J M,et al. The CWI pathway:Regulation of the transcriptional adaptive response to vell wall stress in yeast[J]. Journal of Fungi,2017,4(1):1. doi: 10.3390/jof4010001
[5]	Zhao F,Yang J,Li J,et al. (2020). Multiple cellular responses guarantee yeast survival in presence of the cell membrane/wall interfering agent sodium dodecyl sulfate[J]. Biochemical and Biophysical Research Communications,2020,527(1):276-282. doi: 10.1016/j.bbrc.2020.03.163
[6]	Winzeler E A,Shoemaker D D,Astromoff A,et al. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis[J]. Science,1999,285(5429):901-906. doi: 10.1126/science.285.5429.901
[7]	Ohashi K,Chaleckis R. High levels of Tryptophan reduce cell wall or membrane stress tolerance in Saccharomyces cerevisiae[J]. Bioscience,Biotechnology,and Biochemistry,2021,85(10):2131-2136.
[8]	Li J,Zhang M M,Wan C,et al. Improved cellulase production in recombinant Saccharomyces cerevisiae by disrupting the cell wall protein-encoding gene CWP2[J]. Journal of Bioscience and Bioengineering,2020,129(2):165-171. doi: 10.1016/j.jbiosc.2019.08.012
[9]	Ravishankar A,Pupo A,Gallagher J E G. Resistance mechanisms of Saccharomyces cerevisiae to commercial formulations of glyphosate involve DNA damage repair,the cell cycle,and the cell wall structure[J]. G3,2020,10(6):2043-2056. doi: 10.1534/g3.120.401183
[10]	Feng L,Wang J,Ye D,et al. Yeast population dynamics during spontaneous fermentation of icewine and selection of indigenous Saccharomyces cerevisiae strains for the winemaking in Qilian,China[J]. Journal of the Science of Food and Agriculture,2020,100(15):5385-5394. doi: 10.1002/jsfa.10588
[11]	Hara K Y,Saito M,Kato H,et al. 5-Aminolevulinic acid fermentation using engineered Saccharomyces cerevisiae[J]. Microbial Cell Factories,2019,18(1):194. doi: 10.1186/s12934-019-1242-6

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