CircRNA EZH2 Promotes Proliferation and Migration of Prostate Cancer Cells by Regulating miR-30c-5p

Bin ZHAO; Yuanpeng DUAN; Guoying ZHANG; Chengwei BI; Libo YANG; Zhiyu SHI; Yong YANG; Jianpeng ZHANG; Ting GAO

doi:10.12259/j.issn.2095-610X.S20220731

Volume 43 Issue 7

Jul. 2022

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2022 > 43(7): 25-32

Bin ZHAO, Yuanpeng DUAN, Guoying ZHANG, Chengwei BI, Libo YANG, Zhiyu SHI, Yong YANG, Jianpeng ZHANG, Ting GAO. CircRNA EZH2 Promotes Proliferation and Migration of Prostate Cancer Cells by Regulating miR-30c-5p[J]. Journal of Kunming Medical University, 2022, 43(7): 25-32. doi: 10.12259/j.issn.2095-610X.S20220731

Citation:

Bin ZHAO, Yuanpeng DUAN, Guoying ZHANG, Chengwei BI, Libo YANG, Zhiyu SHI, Yong YANG, Jianpeng ZHANG, Ting GAO. CircRNA EZH2 Promotes Proliferation and Migration of Prostate Cancer Cells by Regulating miR-30c-5p[J]. Journal of Kunming Medical University, 2022, 43(7): 25-32. doi: 10.12259/j.issn.2095-610X.S20220731

Citation:

Bin ZHAO, Yuanpeng DUAN, Guoying ZHANG, Chengwei BI, Libo YANG, Zhiyu SHI, Yong YANG, Jianpeng ZHANG, Ting GAO. CircRNA EZH2 Promotes Proliferation and Migration of Prostate Cancer Cells by Regulating miR-30c-5p[J]. Journal of Kunming Medical University, 2022, 43(7): 25-32. doi: 10.12259/j.issn.2095-610X.S20220731

PDF( 2704 KB)

CircRNA EZH2 Promotes Proliferation and Migration of Prostate Cancer Cells by Regulating miR-30c-5p

doi: 10.12259/j.issn.2095-610X.S20220731

1.
Dept. of Urinary Surgery，Yunnan Provincial Tumor Hospital，Kunming Yunnan 650118
2.
University Hospital，Yunnan University of Finance and Economics，Kunming Yunnan 650021，China

Received Date: 2022-04-27
Available Online: 2022-06-24
Publish Date: 2022-07-14

Abstract

Abstract

Objective To investigate the effect and related mechanism of circRNA EZH2 on proliferation and migration of prostate cancer cells through miR-30c-5p. Methods The expression difference and survival probability of EZH2 in prostate cancer were analyzed according to TCGA database. SiRNA were transfected to silence EZH2 in prostate cancer LNCaP cells, and proliferation and migration of LNCaP cells were detected by CCK-8 assay and wound healing assay, and the expression of miR-30c-5p, JAK1 and PI3K proteins were determined by RT-qPCR and western blot. Results The expression of EZH2 was up-regulated in prostate cancer tissues, and the survival rate of patients with high expression of EZH2 decreased. Compare with normal LNCaP cells, the proliferation and migration of EZH2-silenced LNCaP cells were decreased, miR-30c-5p expression was increased, JAK1 expression was decreased, and PI3K signal was inhibited. Conclusion EZH2 is highly expressed in prostate cancer and can promote the proliferation and migration of LNCaP cells by regulating miR-30c-5p/JAK1 axis and acting on PI3K signaling pathway.
- Prostate cancer,
- CircRNA EZH2,
- miR-30c-5p,
- Proliferation,
- Migration

FullText(HTML)

References(19)

References

[1]	Rebello R J,Oing C,Knudsen K E,et al. Prostate cancer[J]. Nat Rev Dis Primers,2021,7(1):9.
[2]	Swami U,McFarland T R,Nussenzveig R,et al. Advanced prostate cancer:Treatment advances and future directions[J]. Trends Cancer,2020,6(8):702-715.
[3]	Rebbeck,T R. Prostate cancer genetics:Variation by race,ethnicity,and geography[J]. Semin Radiat Oncol,2017,27(1):3-10.
[4]	Wang C,Tan S,Li J,et al. CircRNAs in lung cancer - Biogenesis,function and clinical implication[J]. Cancer Lett,2020,492:106-115.
[5]	Liu Y,Chen S,Zong Z H,et al. CircRNA WHSC1 targets the miR-646/NPM1 pathway to promote the development of endometrial cancer[J]. J Cell Mol Med,2020,24(12):6898-6907.
[6]	Huang,G,Liang M,Liu H,et al. CircRNA hsa_circRNA_104348 promotes hepatocellular carcinoma progression through modulating miR-187-3p/RTKN2 axis and activating Wnt/β-catenin pathway[J]. Cell Death Dis,2020,11(12):1065.
[7]	Wen J,Li X,Ding Y,et al. Lidocaine inhibits glioma cell proliferation,migration and invasion by modulating the circEZH2/miR-181b-5p pathway[J]. Neuroreport,2021,32(1):52-60.
[8]	Zhou Y,Shi H,Du Y,et al. lncRNA DLEU2 modulates cell proliferation and invasion of non-small cell lung cancer by regulating miR-30c-5p/SOX9 axis[J]. Aging (Albany NY),2019,11(18):7386-7401.
[9]	Herichova I,Reis R,Hasakova K,et al. Downregulation of miR-30c-5p expression in colorectal cancer tissue is sex-dependent[J]. Physiol Res,2020,69(Suppl 3):S479-S487.
[10]	Tanaka T,Okada R,Hozaka Y,et al. Molecular pathogenesis of pancreatic ductal adenocarcinoma:impact of miR-30c-5p and miR-30c-2-3p regulation on oncogenic genes[J]. Cancers (Basel),2020,12(10):2731.
[11]	Liu J,Lichtenberg T,Hoadley K A,et al. An integrated TCGA Pan-Cancer clinical data resource to drive high-quality survival outcome analytics[J]. Cell,2018,173(2):400-416.
[12]	Chen B,Lai J,Dai D,et al. JAK1 as a prognostic marker and its correlation with immune infiltrates in breast cancer[J]. Aging (Albany NY),2019,11(23):11124-11135.
[13]	Ma X,Zhao X,Zhang Z,et al. Differentially expressed non-coding RNAs induced by transmissible gastroenteritis virus potentially regulate inflammation and NF-κB pathway in porcine intestinal epithelial cell line[J]. BMC Genomics,2018,19(1):747.
[14]	Gao F,Du Y,Zhang Y,et al. Circ-EZH2 knockdown reverses DDAH1 and CBX3-mediated cell growth and invasion in glioma through miR-1265 sponge activity[J]. Gene,2020,726:144196.
[15]	Yang J,Nie J,Ma X,et al. Targeting PI3K in cancer:mechanisms and advances in clinical trials[J]. Mol Cancer,2019,18(1):26.
[16]	Taylor B S,Schultz N,Hieronymus H,et al. Integrative genomic profiling of human prostate cancer[J]. Cancer Cell,2010,18(1):11-22.
[17]	Tang Y,Pan J,Huang S,et al. Downregulation of miR-133a-3p promotes prostate cancer bone metastasis via activating PI3K/AKT signaling[J]. J Exp Clin Cancer Res,2018,37(1):160.
[18]	Liu J M,Yu C P,Chuang H C,et al. Androgen deprivation therapy for prostate cancer and the risk of autoimmune diseases[J]. Prostate Cancer Prostatic Dis,2019,22(3):475-482.
[19]	Edlind M P,Hsieh A C. PI3K-AKT-mTOR signaling in prostate cancer progression and androgen deprivation therapy resistance[J]. Asian J Androl,2014,16(3):378-386.

Relative Articles(20)

Relative Articles

[1]	Ling WANG, Xiangchuan QIN, Jinqiu LI, Hasim AXIANGU. CD147 Mediates Cervical Cancer Cell Pyroptosis and Proliferation through AIM2 Inflammasome. Journal of Kunming Medical University, 2024, 45(1): 15-21. doi: 10.12259/j.issn.2095-610X.S20240103
[2]	Xiangchuan QIN, Jinqiu LI, Xiaojing HUANG, Kuerban HUTUBIDING·, Hasim AXIANGU·. Effect of HPV E6 on Proliferation，Invasion and Migration of Cervical Cancer Cells Through Rap1 Signaling Pathway. Journal of Kunming Medical University, 2024, 45(9): 9-16. doi: 10.12259/j.issn.2095-610X.S20240902
[3]	Tao HU, Yi WU, Wenda GENG, Yijian ZHANG, Xuan HE, Shanshan LI, Yanbin XIYANG, Liling DENG. Voluntary Exercise Training Attenuated the Proliferation and Growth of Human Breast Cancer with BRCA1 Mutation by Regulating Caspase-3 Activity. Journal of Kunming Medical University, 2023, 44(4): 31-39. doi: 10.12259/j.issn.2095-610X.S20230419
[4]	Yelin ZHANG, Liya MA, Xuhui PENG, Hefeng YANG, Rui SHE. Hsa-let-7a-5p Regulates Proliferation and Apoptosis of Periodontal Ligament Stem Cells. Journal of Kunming Medical University, 2023, 44(10): 47-54. doi: 10.12259/j.issn.2095-610X.S20231028
[5]	Liang ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Yang KE, Wei ZHANG. Effect of miR-29c-3p/IGF1 Molecular Axis on Activation，Proliferation and Apoptosis of Hepatic Stellate Cells. Journal of Kunming Medical University, 2023, 44(9): 7-14. doi: 10.12259/j.issn.2095-610X.S20230926
[6]	Bangqing LIU, Jianfeng LI, Xiaohui LIU, Jinnan ZHANG, Jinping LIANG. MiR-196b Promotes Proliferation and Migration of Lung Adenocarcinoma by Targeting ERG. Journal of Kunming Medical University, 2023, 44(10): 83-91. doi: 10.12259/j.issn.2095-610X.S20231023
[7]	Baoquan WANG, Wei ZHANG, Yuan TIAN, Xifeng LEI, Xu WANG. miR-142-5p Regulates the Proliferation and Metastasis of Gallbladder Carcinoma Cells through CCND1. Journal of Kunming Medical University, 2022, 43(2): 47-53. doi: 10.12259/j.issn.2095-610X.S20220223
[8]	Wei ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Liang ZHANG. miR-125b-5p Regulates HK2 to Inhibit Proliferation and Glycolysis of Gallbladder Cancer Cells. Journal of Kunming Medical University, 2022, 43(12): 23-29. doi: 10.12259/j.issn.2095-610X.S20221206
[9]	Zhoujun LIAO, Shaohua YANG, Lixin LIU, Sheng HU, Yihui CHEN, Qiang KANG, Xiaowen ZHANG. Effect of AK4 on Proliferation and Migration of Intrahepatic Bile Duct Carcinoma Cell HUCCT1. Journal of Kunming Medical University, 2022, 43(6): 1-6. doi: 10.12259/j.issn.2095-610X.S20220611
[10]	Lan YANG, Xiao JIA, Yi-tong JIANG, Qi CUI, Guang-ci LIU, Ying-hong HE. The Effect of Silencing of UBE2C Gene on the Proliferation and Migration of Human Gastric Cancer AGS Cells. Journal of Kunming Medical University, 2021, 42(5): 18-23. doi: 10.12259/j.issn.2095-610X.S20210504
[11]	Li Hui , Ping Qin Rong , Shi Yun Qiang , Wang Chun Hui , Yang Yang , Hu Li Bing , Bi Xiao Fang , Li Jian , Zhong Yi Ming . . Journal of Kunming Medical University, 2020, 41(01): 52-56.
[12]	Pang Yue Wen , Gong Hui Jie , Wang Ling , Shi Jing , Duan JunYao , Feng Ying Ying , Yan Yong Ji . . Journal of Kunming Medical University, 2019, 40(08): 22-27.
[13]	Zhao Bin , Li Rui Qian , Zhang Guo Ying , Lei Yong Hong , Wang Qi Lin , Liu Zhi Min . Overexpression of miR-4328 Inhibits the Proliferation, Migration and Epithelial Mesenchymal Transition of Prostate Cancer Cells. Journal of Kunming Medical University, 2017, 38(11): 29-33.
[14]	Jia Nan Nan . Effect of Gnaq on the Proliferation of SH-SY5Y Cells and Its Mechanism of Action. Journal of Kunming Medical University,
[15]	Ma Yin Rui . . Journal of Kunming Medical University,
[16]	Liu Li Xin . . Journal of Kunming Medical University,
[17]	Wang Hai Feng . . Journal of Kunming Medical University,
[18]	Sun Jian Wei . . Journal of Kunming Medical University,
[19]	Liu Peng Jie . . Journal of Kunming Medical University,
[20]	Liu Jia Xin . . Journal of Kunming Medical University,