CircRNA EZH2 Promotes Proliferation and Migration of Prostate Cancer Cells by Regulating miR-30c-5p

Bin ZHAO; Yuanpeng DUAN; Guoying ZHANG; Chengwei BI; Libo YANG; Zhiyu SHI; Yong YANG; Jianpeng ZHANG; Ting GAO

doi:10.12259/j.issn.2095-610X.S20220731

Volume 43 Issue 7

Jul. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(7): 25-32

Bin ZHAO, Yuanpeng DUAN, Guoying ZHANG, Chengwei BI, Libo YANG, Zhiyu SHI, Yong YANG, Jianpeng ZHANG, Ting GAO. CircRNA EZH2 Promotes Proliferation and Migration of Prostate Cancer Cells by Regulating miR-30c-5p[J]. Journal of Kunming Medical University, 2022, 43(7): 25-32. doi: 10.12259/j.issn.2095-610X.S20220731

Citation:

Bin ZHAO, Yuanpeng DUAN, Guoying ZHANG, Chengwei BI, Libo YANG, Zhiyu SHI, Yong YANG, Jianpeng ZHANG, Ting GAO. CircRNA EZH2 Promotes Proliferation and Migration of Prostate Cancer Cells by Regulating miR-30c-5p[J]. Journal of Kunming Medical University, 2022, 43(7): 25-32. doi: 10.12259/j.issn.2095-610X.S20220731

Citation:

Bin ZHAO, Yuanpeng DUAN, Guoying ZHANG, Chengwei BI, Libo YANG, Zhiyu SHI, Yong YANG, Jianpeng ZHANG, Ting GAO. CircRNA EZH2 Promotes Proliferation and Migration of Prostate Cancer Cells by Regulating miR-30c-5p[J]. Journal of Kunming Medical University, 2022, 43(7): 25-32. doi: 10.12259/j.issn.2095-610X.S20220731

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CircRNA EZH2 Promotes Proliferation and Migration of Prostate Cancer Cells by Regulating miR-30c-5p

doi: 10.12259/j.issn.2095-610X.S20220731

1.
Dept. of Urinary Surgery，Yunnan Provincial Tumor Hospital，Kunming Yunnan 650118
2.
University Hospital，Yunnan University of Finance and Economics，Kunming Yunnan 650021，China

Received Date: 2022-04-27
Available Online: 2022-06-24
Publish Date: 2022-07-14

Abstract

Abstract

Objective To investigate the effect and related mechanism of circRNA EZH2 on proliferation and migration of prostate cancer cells through miR-30c-5p. Methods The expression difference and survival probability of EZH2 in prostate cancer were analyzed according to TCGA database. SiRNA were transfected to silence EZH2 in prostate cancer LNCaP cells, and proliferation and migration of LNCaP cells were detected by CCK-8 assay and wound healing assay, and the expression of miR-30c-5p, JAK1 and PI3K proteins were determined by RT-qPCR and western blot. Results The expression of EZH2 was up-regulated in prostate cancer tissues, and the survival rate of patients with high expression of EZH2 decreased. Compare with normal LNCaP cells, the proliferation and migration of EZH2-silenced LNCaP cells were decreased, miR-30c-5p expression was increased, JAK1 expression was decreased, and PI3K signal was inhibited. Conclusion EZH2 is highly expressed in prostate cancer and can promote the proliferation and migration of LNCaP cells by regulating miR-30c-5p/JAK1 axis and acting on PI3K signaling pathway.
- Prostate cancer,
- CircRNA EZH2,
- miR-30c-5p,
- Proliferation,
- Migration

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References(19)

References

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