Polyethylene Glycol 4000 Lowers Serum Uric Acid in Uricase-deficient Rats

Wan QIN; Yalin QI; Yinfang GAO; Nan FAN; Yu YUN; Weigang DUAN

doi:10.12259/j.issn.2095-610X.S20220801

Volume 43 Issue 8

Jul. 2022

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Wan QIN, Yalin QI, Yinfang GAO, Nan FAN, Yu YUN, Weigang DUAN. Polyethylene Glycol 4000 Lowers Serum Uric Acid in Uricase-deficient Rats[J]. Journal of Kunming Medical University, 2022, 43(8): 1-6. doi: 10.12259/j.issn.2095-610X.S20220801

Citation:

Wan QIN, Yalin QI, Yinfang GAO, Nan FAN, Yu YUN, Weigang DUAN. Polyethylene Glycol 4000 Lowers Serum Uric Acid in Uricase-deficient Rats[J]. Journal of Kunming Medical University, 2022, 43(8): 1-6. doi: 10.12259/j.issn.2095-610X.S20220801

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Polyethylene Glycol 4000 Lowers Serum Uric Acid in Uricase-deficient Rats

doi: 10.12259/j.issn.2095-610X.S20220801

Wan QIN^{1, 2},
Yalin QI¹,
Yinfang GAO³,
Nan FAN³,
Yu YUN³,
Weigang DUAN^{1
,
,}

1.
School of Basic Medicine
2.
School of Chinese Pharmacy，Yunnan University of Chinese Medicine，Kunming Yunnan 650500
3.
School of Basic Medicine，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2022-04-20
Available Online: 2022-07-20
Publish Date: 2022-07-28

Abstract

Abstract

Objective To explore serum uric acid （SUA） lowering effect of polyethylene glycol 4000 （PEG4000） on male Kunming-DY rats （uricase-deficient rats）. Methods A total of 24 45-day male Kunming-DY rats were selected and divided into control group （n = 6） and treatment group: high （10.5 g/L）, medium （5.25 g/L） and low （2.625 g/L） PEG4000 dose groups with 6 rats in each group. The control group drank freely and the treatment group drank freely for 1 week. Before and after the experiment, the tail was cut and blood was collected, and the serum, urine and feces of the rats were collected for 24 hours. The food and water intake for 24 hours were recorded, and the main tissues and organs of the rats were harvested. The contents of uric acid, blood urea nitrogen and creatinine in serum samples, uric acid in excreta and uric acid in tissues and organs were tested, and the changes of indexes in control group and treatment group were compared. Results The blood uric acid in the control group was higher than 70 µg/mL, while PEG4000 （10.5 and 5.25 g/L） reduced it to 50-60 g/ml, and the difference was statistically significant （P < 0.05）. Compared with the control group, the fecal excretion of PEG4000 （10.5, 5.25, 2.625 g/L） groups increased significantly （P < 0.05）. Compared with the control group, the blood urea nitrogen of PEG4000 （5.25 g/L） in the treatment group also decreased significantly, and the difference was statistically significant （P < 0.05）. Compared with the control group, PEG4000 （5.25 g/L） group decreased the uric acid content in tissues （heart, liver, spleen, lung, kidney and adrenal gland）, and the difference was statistically significant （P < 0.05）; PEG4000 （2.625 g/L） decreased the uric acid in tissues （liver, spleen, kidney and adrenal gland）, and the difference was statistically significant （P < 0.05）. Conclusion Oral PEG4000 solution can reduce uric acid, which is related to the increase of defecation. Oral PEG4000 solution can reduce the burden of uric acid in kidney.
- Kunming-DY rat,
- Uricase deficiency,
- Hyperuricemia,
- PEG4000,
- Serum uric acid lowering

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