Protective Effect of Interleukin-4 in Lipopolysaccharide-induced Acute Lung Injury Models

Kun ZHAO; Yun XIAO; Chun YANG; Zhiling YAN; Minna DONG; Bingquan XIANG; Mingyao XIAO

doi:10.12259/j.issn.2095-610X.S20220805

Volume 43 Issue 8

Jul. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(8): 41-46

Kun ZHAO, Yun XIAO, Chun YANG, Zhiling YAN, Minna DONG, Bingquan XIANG, Mingyao XIAO. Protective Effect of Interleukin-4 in Lipopolysaccharide-induced Acute Lung Injury Models[J]. Journal of Kunming Medical University, 2022, 43(8): 41-46. doi: 10.12259/j.issn.2095-610X.S20220805

Citation:

Kun ZHAO, Yun XIAO, Chun YANG, Zhiling YAN, Minna DONG, Bingquan XIANG, Mingyao XIAO. Protective Effect of Interleukin-4 in Lipopolysaccharide-induced Acute Lung Injury Models[J]. Journal of Kunming Medical University, 2022, 43(8): 41-46. doi: 10.12259/j.issn.2095-610X.S20220805

Citation:

Kun ZHAO, Yun XIAO, Chun YANG, Zhiling YAN, Minna DONG, Bingquan XIANG, Mingyao XIAO. Protective Effect of Interleukin-4 in Lipopolysaccharide-induced Acute Lung Injury Models[J]. Journal of Kunming Medical University, 2022, 43(8): 41-46. doi: 10.12259/j.issn.2095-610X.S20220805

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Protective Effect of Interleukin-4 in Lipopolysaccharide-induced Acute Lung Injury Models

doi: 10.12259/j.issn.2095-610X.S20220805

1.
Dept. of Critical Care Medicine
2.
Dept. of Gynecological Oncology，The 3rd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650118
3.
Dept. of Emergency，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2022-03-28
Available Online: 2022-07-20
Publish Date: 2022-07-28

Abstract

Abstract

Objective To investigate the protective effect of interleukin-4 （IL-4） on acute lung injury （ALI）. Methods ALI cell models were developed using lipopolysaccharide （LPS） to induce A549 cells. The model was treated with different concentrations of IL-4 （0.1 μg/mL, 1 μg/mL, 10 μg/mL） for different durations. Then the apoptosis rate of A549 cells was detected by flow cytometry, and the secretion of IL-1, IL-6, IL-10, TNF-α, TNF-γ of A549 cells was determined by ELISA. Results IL-4 reduced the apoptosis rate of A549 cells and inhibited the expression of Caspase3 in ALI model （P < 0.05）. The effect was enhanced with the increase of IL-4 concentration and the extension of intervention time. IL-4 promoted the expression of Bcl-2 （P < 0.05）, and the best effect was achieved when the concentration of IL-4 was 1 μg/mL for 12 h. Under the above conditions, IL-4 decreased IL-1, IL-6, TNF-α, TNF-γ and increased IL-10 in A549 cells in ALI model （P < 0.05）. Conclusion IL-4 can positively regulate acute lung injury by improving cytokine secretion in ALI in vitro model.
- Acute lung injury,
- Interleukin-4,
- Lipopolysaccharide,
- Regulatory T cells,
- Cytokines

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References(20)

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