Effect of Cathelicidin LL-37 on Transmembrane Barrier Function of Urothelial Cells

Tongxin YANG; Guang WANG; Rui XU; Siwei SU; Kewei FANG; Jianhe LIU; Jiongming LI

doi:10.12259/j.issn.2095-610X.S20220806

Volume 43 Issue 8

Jul. 2022

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Tongxin YANG, Guang WANG, Rui XU, Siwei SU, Kewei FANG, Jianhe LIU, Jiongming LI. Effect of Cathelicidin LL-37 on Transmembrane Barrier Function of Urothelial Cells[J]. Journal of Kunming Medical University, 2022, 43(8): 34-40. doi: 10.12259/j.issn.2095-610X.S20220806

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Effect of Cathelicidin LL-37 on Transmembrane Barrier Function of Urothelial Cells

doi: 10.12259/j.issn.2095-610X.S20220806

Dept. of Urology，The 2nd Affiliated Hospital of Kunming Medical University， Kunming Yunnan 650101，China

Received Date: 2022-04-20
Available Online: 2022-07-23
Publish Date: 2022-07-28

Abstract

Abstract

Objective To explore the specific effect of LL-37 on the destruction of the transmembrane barrier function of bladder urothelial cells and develop a cellular experimental model suitable for studying interstitial cystitis （IC） in vitro. Methods Human urothelial immortalized cells SV-HUC-1 were treated with LL-37 at different concentrations. The transepithelial electrical resistance （TEER） was measured by a transepithelial resistor, and the cell proliferative activity was detected by CCK-8 kit. Cell cycle and calcium ion concentration were detected by flow cytometry. The expression levels of heparan sulfate, a key component of glycosaminoglycan （GAGs）, were detected by RT-qPCR and WB. Results In vitro measurement showed that 100 and 200 μg/mL of LL-37 significantly inhibited the TEER and destroyed the transmembrane barrier function of SV-HUC-1. The CCK-8 test and PI staining flow cytometry results showed that LL-37 above 100 μg/mL could significantly reduce the proliferation activity of SV-HUC-1 and increase the proportion of SV-HUC-1 in the G0/G1 phase （P < 0.05）, suggesting that cell proliferation was significantly inhibited. Further flow cytometry analysis showed that the calcium ion concentration in SV-HUC-1treated with LL-37 was significantly increased （P < 0.05）, and the increase was related to LL-37 concentration. RT-qPCR and WB assays confirmed significant up-regulation of heparan sulfate, a key GAGs component, in the LL-37-treated SV-HUC-1 （P < 0.05）. Conclusion The cathelicidin LL-37 can inhibit the expression of GAGs and destroy the cell proliferation and transmembrane barrier function of SV-HUC-1.
- Interstitial cystitis,
- Cathelicidin LL-37,
- Urothelium cells,
- Transmembrane barrier,
- Glycosaminoglycan

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References

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