Breviscapine Regulates the mRNA and Protein Levels of CINC-1 and CINC-2 in the Right Ventricle of Rats with Acute Pulmonary Embolism

Zhenkun LI; Tuanli BAI; Linyu YANG; Ying Wang

doi:10.12259/j.issn.2095-610X.S20221002

Volume 43 Issue 10

Oct. 2022

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Zhenkun LI, Tuanli BAI, Linyu YANG, Ying Wang. Breviscapine Regulates the mRNA and Protein Levels of CINC-1 and CINC-2 in the Right Ventricle of Rats with Acute Pulmonary Embolism[J]. Journal of Kunming Medical University, 2022, 43(10): 28-34. doi: 10.12259/j.issn.2095-610X.S20221002

Citation:

Zhenkun LI, Tuanli BAI, Linyu YANG, Ying Wang. Breviscapine Regulates the mRNA and Protein Levels of CINC-1 and CINC-2 in the Right Ventricle of Rats with Acute Pulmonary Embolism[J]. Journal of Kunming Medical University, 2022, 43(10): 28-34. doi: 10.12259/j.issn.2095-610X.S20221002

Citation:

Zhenkun LI, Tuanli BAI, Linyu YANG, Ying Wang. Breviscapine Regulates the mRNA and Protein Levels of CINC-1 and CINC-2 in the Right Ventricle of Rats with Acute Pulmonary Embolism[J]. Journal of Kunming Medical University, 2022, 43(10): 28-34. doi: 10.12259/j.issn.2095-610X.S20221002

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Breviscapine Regulates the mRNA and Protein Levels of CINC-1 and CINC-2 in the Right Ventricle of Rats with Acute Pulmonary Embolism

doi: 10.12259/j.issn.2095-610X.S20221002

1.
Pulmonary and Critical Care Medicine，The Second Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101
2.
Second Clinical Medical Colleges of Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2022-05-27
Available Online: 2022-10-08
Publish Date: 2022-10-31

Abstract

Abstract

Objective To observe whether breviscapine has any effect on the mRNA and protein levels of CINC-1 and CINC-2 in the right ventricle of rats with pulmonary embolism. Methods The rat model of pulmonary embolism was replicated, and rats in the breviscapine low-dose, high-dose and low-molecular-weight heparin groups were given intraperitoneal injection of corresponding drugs, lung tissue samples were collected for pathological detection of pulmonary embolism, and right ventricular tissue samples were determined by Q-PCR and Western blot to determine CINC-1, CINC-2 gene and protein levels in each group. Results Compared with the sham operation group, the gene expression of CINC-1 and CINC-2 in the model group was significantly upregulated （P < 0.01）. The low-dose, high-dose and low-molecular-weight heparin groups of breviscapine could significantly down-regulate the gene expression of CINC-1 and CINC-2 （P < 0.01）. The high-dose breviscapine group was better than the low-molecular-weight heparin group in down-regulating CINC-1 gene level （P < 0.05）. Compared with the sham operation group, the protein expressions of CINC-1 and CINC-2 in the model group were significantly increased （P < 0.01）. The low-dose and high-dose groups of breviscapine could significantly down-regulate the protein level of CINC-1 （P < 0.01）, and the high-dose group of breviscapine was better than the low-molecular-weight heparin group in down-regulating the protein expression of CINC-1 （P < 0.05）. And the high dose of breviscapine can significantly down-regulate the protein level of CINC-2 （P < 0.01）. Conclusion Breviscapine may play an anti-inflammatory role in pulmonary embolism by down-regulating the gene and protein expression of CINC-1 and CINC-2 in the right ventricle of rats with acute pulmonary embolism.
- Breviscapine,
- Pulmonary embolism,
- CINC-1,
- CINC-2

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References(14)

References

[1]	Duffett L,Castellucci LA,Forgie MA. Pulmonary embolism:update on management and controversies[J]. BMJ,2020,370:2177. doi: 10.1136/bmj.m2177
[2]	Watts JA,Lee YY,Gellar MA,et al. Proteomics of microparticles after experimental pulmonary embolism[J]. Thromb Res,2012,130(1):122-128. doi: 10.1016/j.thromres.2011.09.016
[3]	Shibata F. The role of rat cytokine-induced neutrophil chemoattractants (CINCs) in inflamation[J]. Akugaku Zasshi,2002,122(4):263-268. doi: 10.1248/yakushi.122.263
[4]	Zagorski J,Gellar M A,Obraztsova M,et al. Inhibition of CINC-1 decreases right ventricular damage caused by experimental pulmonary embolism in rats[J]. J Immunol,2007,179(11):7820-7826. doi: 10.4049/jimmunol.179.11.7820
[5]	Li Y,Li S,Li D. Breviscapine Alleviates Cognitive Impairments Induced by Transient Cerebral Ischemia/Reperfusion through Its Anti-Inflammatory and Anti-Oxidant Properties in a Rat[J]. ACS Chem Neurosci,2020,11(24):4489-4498.
[6]	Wang C,Li Y,Gao S,et al. Breviscapine Injection Improves the Therapeutic Effect of Western Medicine on Angina Pectoris Patients[J]. PLoS One,2015,10(6):e0129969. doi: 10.1371/journal.pone.0129969
[7]	Li Z,Lin H,Dong Z,et al. Protective effect of breviscapine in acute pulmonary embolism rats via regulation of MCP-1 and IL-13[J]. Journal of Cellular and Molecular Medicine,2018,22(12):6405-6407. doi: 10.1111/jcmm.13871
[8]	Jarman AF,Mumma BE,Singh KS,et al. Crucial considerations:Sex differences in the epidemiology,diagnosis,treatment,and outcomes of acute pulmonary embolism in non-pregnant adult patients[J]. J Am Coll Emerg Physicians Open,2021,2(1):e12378.
[9]	Cohen AT,Katholing A,Rietbrock S,et al. Epidemiology of first and recurrent venous thromboembolism in patients with active cancer. A population-based cohort study[J]. Korean J Intern Med,2019,34(5):1125-1135. doi: 10.3904/kjim.2018.097
[10]	Bircan HA,Alanoglu EG. Massive Pulmonary Embolism in a Patient with Heparin Induced Thrombocytopenia:Successful Treatment with Dabigatran[J]. Eurasian J Med,2016,48(1):65-68. doi: 10.5152/eurasianjmed.2015.95
[11]	Condliffe R,Elliot CA,Hughes RJ,et al. Management dilemmas in acute pulmonary embolism[J]. Thorax,2014,69(2):174-180. doi: 10.1136/thoraxjnl-2013-204667
[12]	Adegunsoye A,Balachandran J. Inflammatory response mechanisms exacerbating hypoxemia in coexistent pulmonary fibrosis and sleep apnea[J]. Mediators Inflamm,2015,2015:510105.
[13]	Watts JA,Zagorski J,Gellar MA et al. Cardiac inflammation contributes to right ventricular dysfunction following experimental pulmonary embolism in rats[J]. J Mol Cell Cardiol,2006,41(2):296-307. doi: 10.1016/j.yjmcc.2006.05.011
[14]	Pilarczyk K,Renner J,Haake N. Acute right heart failure on the intensive care unit:Pathophysiology,monitoring and management[J]. Med Klin Intensivmed Notfmed,2019,114(6):567-588. doi: 10.1007/s00063-019-0603-6

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