Zhenkun LI, Tuanli BAI, Linyu YANG, Ying Wang. Breviscapine Regulates the mRNA and Protein Levels of CINC-1 and CINC-2 in the Right Ventricle of Rats with Acute Pulmonary Embolism[J]. Journal of Kunming Medical University, 2022, 43(10): 28-34. doi: 10.12259/j.issn.2095-610X.S20221002
Citation: Zhenkun LI, Tuanli BAI, Linyu YANG, Ying Wang. Breviscapine Regulates the mRNA and Protein Levels of CINC-1 and CINC-2 in the Right Ventricle of Rats with Acute Pulmonary Embolism[J]. Journal of Kunming Medical University, 2022, 43(10): 28-34. doi: 10.12259/j.issn.2095-610X.S20221002

Breviscapine Regulates the mRNA and Protein Levels of CINC-1 and CINC-2 in the Right Ventricle of Rats with Acute Pulmonary Embolism

doi: 10.12259/j.issn.2095-610X.S20221002
  • Received Date: 2022-05-27
    Available Online: 2022-10-08
  • Publish Date: 2022-10-31
  •   Objective  To observe whether breviscapine has any effect on the mRNA and protein levels of CINC-1 and CINC-2 in the right ventricle of rats with pulmonary embolism.   Methods  The rat model of pulmonary embolism was replicated, and rats in the breviscapine low-dose, high-dose and low-molecular-weight heparin groups were given intraperitoneal injection of corresponding drugs, lung tissue samples were collected for pathological detection of pulmonary embolism, and right ventricular tissue samples were determined by Q-PCR and Western blot to determine CINC-1, CINC-2 gene and protein levels in each group.   Results  Compared with the sham operation group, the gene expression of CINC-1 and CINC-2 in the model group was significantly upregulated (P < 0.01). The low-dose, high-dose and low-molecular-weight heparin groups of breviscapine could significantly down-regulate the gene expression of CINC-1 and CINC-2 (P < 0.01). The high-dose breviscapine group was better than the low-molecular-weight heparin group in down-regulating CINC-1 gene level (P < 0.05). Compared with the sham operation group, the protein expressions of CINC-1 and CINC-2 in the model group were significantly increased (P < 0.01). The low-dose and high-dose groups of breviscapine could significantly down-regulate the protein level of CINC-1 (P < 0.01), and the high-dose group of breviscapine was better than the low-molecular-weight heparin group in down-regulating the protein expression of CINC-1 (P < 0.05). And the high dose of breviscapine can significantly down-regulate the protein level of CINC-2 (P < 0.01).   Conclusion  Breviscapine may play an anti-inflammatory role in pulmonary embolism by down-regulating the gene and protein expression of CINC-1 and CINC-2 in the right ventricle of rats with acute pulmonary embolism.
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