Effect of Panax Noto-ginseng Saponins on the Proliferation of Human Stem Cells from Apical Papilla

Shibing QIAN; Mingyao MENG; Hongbin YU; Kaiwen DUAN; Changquan LI; Zhigang XIA

doi:10.12259/j.issn.2095-610X.S20221018

Volume 43 Issue 10

Oct. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(10): 22-27

Shibing QIAN, Mingyao MENG, Hongbin YU, Kaiwen DUAN, Changquan LI, Zhigang XIA. Effect of Panax Noto-ginseng Saponins on the Proliferation of Human Stem Cells from Apical Papilla[J]. Journal of Kunming Medical University, 2022, 43(10): 22-27. doi: 10.12259/j.issn.2095-610X.S20221018

Citation:

Shibing QIAN, Mingyao MENG, Hongbin YU, Kaiwen DUAN, Changquan LI, Zhigang XIA. Effect of Panax Noto-ginseng Saponins on the Proliferation of Human Stem Cells from Apical Papilla[J]. Journal of Kunming Medical University, 2022, 43(10): 22-27. doi: 10.12259/j.issn.2095-610X.S20221018

Citation:

Shibing QIAN, Mingyao MENG, Hongbin YU, Kaiwen DUAN, Changquan LI, Zhigang XIA. Effect of Panax Noto-ginseng Saponins on the Proliferation of Human Stem Cells from Apical Papilla[J]. Journal of Kunming Medical University, 2022, 43(10): 22-27. doi: 10.12259/j.issn.2095-610X.S20221018

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Effect of Panax Noto-ginseng Saponins on the Proliferation of Human Stem Cells from Apical Papilla

doi: 10.12259/j.issn.2095-610X.S20221018

1.
Dept. of Stomatology，Yan’an Hospital，Kunming City，The Affiliated Yan’an Hospital，Kunming Medical University，Kunming Yunnan 650051
2.
Central Laboratory，Yan’an Hospital, Kunming City，The Affiliated Yan’an Hospital，Kunming Medical University， Kunming Yunnan 650051，China

Received Date: 2022-06-11
Available Online: 2022-10-09
Publish Date: 2022-10-31

Abstract

Abstract

Objective To explore the effects of which Panax noto-ginseng saponins （PNS） influence the proliferation of human stem cells from apical papilla （hSCAPs）. Methods We collected apical papilla and separated primary cells with wall methods. Flow cytometry was used to identify the surface markers of hSCAPs, the multi-directional-differentiation potential of hSCAPs was identified by inducing bone and fat differentiation. PNS-culture-medium with 25 mg/L, 50 mg/L, 100 mg/L, and 200 mg /L was prepared, CCK-8 method was used to detect the proliferation rate of hSCAPs under different concentrations of PNS, and the changes of the cell cycle at the best proliferation concentration were observed. Results hSCAPs was cultivated successfully by primary separation and belong to the source of mesenchymal stem cells. The related surface markers were highly expressed and induced to fat differentiation and bone differentiation. Compared with the control and other experimental groups, PNS with 25 mg/L promoted highly hSCAPs proliferation. The difference was statistically significant （P < 0.05）. Conclusions PNS with 25 mg/L has the best effect for boosting hSCAPs proliferation abilities. It is prompted that hSCAPs has a strong sensitivity to PNS, that is, a low concentration can significantly promote the proliferation of hSCAPs.
- Panax notoginseng saponins,
- Human apical papilla stem cells,
- Cell proliferation,
- Cell cycle

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References(19)

References

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