Second-generation Sequencing Study of Trophoblast Cells in vitro Stimulated by Different Concentrations of Vitamin D

Tong XU; Yantuanjin MA; Yuhang ZHANG; Qiuyue HE; Wei HUANG; Mengxin LU; Jian TANG; Jianping HE; Guoqing JIANG; Yuan QIAN

doi:10.12259/j.issn.2095-610X.S20221021

Volume 43 Issue 10

Oct. 2022

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Tong XU, Yantuanjin MA, Yuhang ZHANG, Qiuyue HE, Wei HUANG, Mengxin LU, Jian TANG, Jianping HE, Guoqing JIANG, Yuan QIAN. Second-generation Sequencing Study of Trophoblast Cells in vitro Stimulated by Different Concentrations of Vitamin D[J]. Journal of Kunming Medical University, 2022, 43(10): 1-9. doi: 10.12259/j.issn.2095-610X.S20221021

Citation:

Tong XU, Yantuanjin MA, Yuhang ZHANG, Qiuyue HE, Wei HUANG, Mengxin LU, Jian TANG, Jianping HE, Guoqing JIANG, Yuan QIAN. Second-generation Sequencing Study of Trophoblast Cells in vitro Stimulated by Different Concentrations of Vitamin D[J]. Journal of Kunming Medical University, 2022, 43(10): 1-9. doi: 10.12259/j.issn.2095-610X.S20221021

Citation:

Tong XU, Yantuanjin MA, Yuhang ZHANG, Qiuyue HE, Wei HUANG, Mengxin LU, Jian TANG, Jianping HE, Guoqing JIANG, Yuan QIAN. Second-generation Sequencing Study of Trophoblast Cells in vitro Stimulated by Different Concentrations of Vitamin D[J]. Journal of Kunming Medical University, 2022, 43(10): 1-9. doi: 10.12259/j.issn.2095-610X.S20221021

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Second-generation Sequencing Study of Trophoblast Cells in vitro Stimulated by Different Concentrations of Vitamin D

doi: 10.12259/j.issn.2095-610X.S20221021

Tong XU^{1, 2, 3},
Yantuanjin MA^{1, 2, 3},
Yuhang ZHANG^{1, 2, 3},
Qiuyue HE^{1, 2, 3},
Wei HUANG^{1, 2, 3},
Mengxin LU⁴,
Jian TANG⁴,
Jianping HE^{4, 5},
Guoqing JIANG⁶,
Yuan QIAN^{1, 2, 3, 4, 5, 6
,
,}

1.
Key Laboratory of Laboratory Medicine，Yunnan Kunming 650032
2.
Clinical Medical Research Center of Medical Laboratory，Yunnan Kunming 650032
3.
Dept. of Medical Laboratory，The First Affiliated Hospital of Kunming Medical University，Yunnan Kunming 650032
4.
Center of Medical Genetics and Prenatal Diagnosis，Kunming Maternal and Child Health Hospital，Yunnan Kunming 650032
5.
Joint Key Laboratory of National Clinical Medical Research Center for Obstetrics and Gynecological Diseases （Yunnan），Kunming Maternal and Child Health Hospital，Yunnan Kunming 650032
6.
Dept. of Obstetrics and Gynecology，The First Affiliated Hospital of Kunming Medical University，Yunnan Kunming 650032，China

Received Date: 2022-08-16
Available Online: 2022-10-09
Publish Date: 2022-10-31

Abstract

Abstract

Objective To screen differential expression gene （DEG） in placental trophoblasts stimulated by vitamin D by second-generation sequencing technology, and to analyze their potential role in the pathogenesis of adverse pregnancy outcomes caused by vitamin D deficiency. Methods The second-generation sequencing analysis was used to detect mRNA expression in placental trophoblasts stimulated by different concentrations of vitamin D （0.1, 1, 10, 100 nm） to screen out DEG, GO and KEGG enrichment analysis was applied, and PPI network gene screening center was constructed. Results Gene expression in four different concentrations of vitamin D stimulated cells of the samples and normal control samples without vitamin D are as follows: a total of 354 DEG were obtained in VitD 0.1 vs Control group, including 242 up-regulated and 112 down-regulated; a total of 320 DEG were obtained in VitD 1 vs Control group, including 216 up-regulated and 104 down-regulated; a total of 374 DEG were obtained in VitD 10 vs Control group, including 277 up-regulated and 97 down-regulated; a total of 300 DEG were obtained in VitD 100 vs Control group, including 151 up-regulated and 149 down-regulated. At last, the intersection of four groups of DEG was taken, and nine common DEGs were obtained. GO analysis showed that biological processes are mainly concentrated in the collagen fibrils tissue, cell differentiation, cell chemotaxis, cell differentiation, the regulation of the negative control, signal receptor activity, outside the cell structure, extracellular matrix organization, protein secretion, adjusting the enrichment of the cellular elements in collagen trimer compounds, proteins of the extracellular matrix, the top part of the cell and collagen trimer; Adjust the molecular function of enrichment in receptor ligands, receptor modulators, protein bridge, the activity of cytokines. KEGG analysis showed significant enrichment in stimulating nerve receptor-ligand interactions, cell adhesion molecules, gastric cancer, cell factor receptor interactions,produce IgA intestinal immune network, inflammatory bowel disease, malaria, amoebiasis and PI3K-Akt signaling pathway. The PPI network identified 17 hub genes, including COL1A2, ACTA2, S100A4, TAGLN, CSF1R, TLR4, TNFSF13B, FTCD, APOBEC3G, IL6, IGF1, PDGFRBTGFB2, BGLAP, COL4A4, COL8A1 and COL11A1. Conclusions COL1A2, ACTA2, S100A4, TAGLN, CSF1R, TLR4, TNFSF13B, FTCD, APOBEC3G, IL6, IGF1, PDGFRBTGFB2, BGLAP, COL4A4, COL8A1 and COL11A1 may be linked to vitamin D deficiency caused by the occurrence and development of adverse pregnancy outcomes, and provide potential therapeutic targets adverse pregnancy outcomes caused by vitamin D deficiency.
- Vitamin D,
- Trophoblast cells,
- Differentially expressed genes

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