Experimental Study on the Expression of mTOR in TMJOA Condylar Cartilage of SD Rats

Zihan WANG; Gaiying YE; Tao ZHAO; Jun ZHANG; Yu HU

doi:10.12259/j.issn.2095-610X.S20221033

Volume 43 Issue 10

Oct. 2022

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Zihan WANG, Gaiying YE, Tao ZHAO, Jun ZHANG, Yu HU. Experimental Study on the Expression of mTOR in TMJOA Condylar Cartilage of SD Rats[J]. Journal of Kunming Medical University, 2022, 43(10): 10-15. doi: 10.12259/j.issn.2095-610X.S20221033

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Zihan WANG, Gaiying YE, Tao ZHAO, Jun ZHANG, Yu HU. Experimental Study on the Expression of mTOR in TMJOA Condylar Cartilage of SD Rats[J]. Journal of Kunming Medical University, 2022, 43(10): 10-15. doi: 10.12259/j.issn.2095-610X.S20221033

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Experimental Study on the Expression of mTOR in TMJOA Condylar Cartilage of SD Rats

doi: 10.12259/j.issn.2095-610X.S20221033

School of Stomatology，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2022-07-24
Publish Date: 2022-10-25

Abstract

Abstract

Objective To investigate the expression of mTOR in TMJOA condylar cartilage of SD rats. Methods The model of TMJOA in SD rats was established by unilateral mastication, and the corresponding sham-operation group was established. TMJOA model was established by unilateral mastication.The right condyle was taken 2, 4 and 8 weeks after modeling. HE staining and safranine o-solid green staining were used for pathological examination; The expressions of Sox9 and mTOR were detected by immunohistochemical staining. Results Hematoxylin-eosin （HE） staining and saffron o-green staining showed that the condylar cartilage of the control group had clear structure, smooth and intact surface, and the proteoglycans were evenly distributed. In the experimental group （TMD group）, obvious degenerative changes of condylar cartilage were observed over time （P < 0.05）, and the lesions were the highest in 4 W. Immunohistochemical staining showed that the expression level of Sox9 in the condylar cartilage of the experimental group decreased successively at 2 W （ P < 0.01）, 4 W （ P < 0.05） and 8 W （ P < 0.05）, and were lower than that of the control group. The expression level of mTOR decreased at 2 W （ P < 0.05） and 4 W （ P < 0.001）, but increased relatively at 8 W （ P < 0.05） . Conclusion With the progress of inflammation, mTOR in TMJOA condylar cartilage of SD rats shows a trend of early down-regulation and late up-regulation, which plays a key role in transforming protective autophagy into destructive apoptosis.
- Temporomandibular Joint,
- Arthritis,
- Cartilage degeneration,
- TOR serine-Threonine Kinases（mTOR）,
- Autophagy

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References(16)

References

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