Effects of Modulation of CDC42 Activity in Hippocampal Neurons on Memory and Anxiety Behaviors of Mice

Chaojie ZOU; Yikai TANG; Shuwen DU; Yuqi CHENG

doi:10.12259/j.issn.2095-610X.S20221105

Volume 43 Issue 11

Nov. 2022

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Chaojie ZOU, Yikai TANG, Shuwen DU, Yuqi CHENG. Effects of Modulation of CDC42 Activity in Hippocampal Neurons on Memory and Anxiety Behaviors of Mice[J]. Journal of Kunming Medical University, 2022, 43(11): 1-8. doi: 10.12259/j.issn.2095-610X.S20221105

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Effects of Modulation of CDC42 Activity in Hippocampal Neurons on Memory and Anxiety Behaviors of Mice

doi: 10.12259/j.issn.2095-610X.S20221105

1.
Dept. of Psychiatry，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032
2.
School of Life Sciences，McGovern Institute for Brain Research，Tsinghua University，Beijing 100084，China

Received Date: 2022-06-15
Available Online: 2022-10-19
Publish Date: 2022-11-14

Abstract

Abstract

Objective To establish an animal model to investigate the relationship between cell division cycle 42 （CDC42） and cognitive deficits in hippocampal neurons. Methods Adenovirus with CDC42 mutant gene was packaged to infect excitatory neurons in the hippocampus of healthy mice by microinjection. The cognitive phenotypes of mice under various behavioral patterns were detected. Results Adenovirus specifically enhanced or decreased the expression activity of CDC42 protein in excitatory neurons in the hippocampus. After the inhibition of CDC42 protein in the hippocampus, the conditioned memory of the mice decreased ,significantly at 24 hours and 96 hours, and enhancing CDC42 activity in the hippocampus made mice show an obvious anxiety behavior. However, neither enhancement nor inhibition of the CDC42 activity in the hippocampus had a significant effect on social behavior （P > 0.05）. Conclusions The mice with altered CDC42 activity in hippocampal excitatory neurons may present a fear conditioning memory defect and significant anxiety behavior, this may suggest the relationship between cytoskeletal protein CDC42 and cognitive phenotypes. This regulatory model may provide new methods and ideas for the establishment of research models for mental disorders such as SZ and CDC42 is expected to become a new molecular target for the treatment of memory impairment or anxiety.
- CDC42,
- Schizophrenia,
- Memory impairment,
- Anxiety

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References(25)

References

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