Study on Differential Expression Profile of lncRNA between Concurrent Chemoradioresistant Nasopharyngeal Carcinoma Cell Lines and Parental Cell Lines

Qiuyan LIU; Wei XIONG; Cheng LI

doi:10.12259/j.issn.2095-610X.S20221108

Volume 43 Issue 11

Nov. 2022

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2022 > 43(11): 77-84

Qiuyan LIU, Wei XIONG, Cheng LI. Study on Differential Expression Profile of lncRNA between Concurrent Chemoradioresistant Nasopharyngeal Carcinoma Cell Lines and Parental Cell Lines[J]. Journal of Kunming Medical University, 2022, 43(11): 77-84. doi: 10.12259/j.issn.2095-610X.S20221108

Citation:

Qiuyan LIU, Wei XIONG, Cheng LI. Study on Differential Expression Profile of lncRNA between Concurrent Chemoradioresistant Nasopharyngeal Carcinoma Cell Lines and Parental Cell Lines[J]. Journal of Kunming Medical University, 2022, 43(11): 77-84. doi: 10.12259/j.issn.2095-610X.S20221108

Citation:

Qiuyan LIU, Wei XIONG, Cheng LI. Study on Differential Expression Profile of lncRNA between Concurrent Chemoradioresistant Nasopharyngeal Carcinoma Cell Lines and Parental Cell Lines[J]. Journal of Kunming Medical University, 2022, 43(11): 77-84. doi: 10.12259/j.issn.2095-610X.S20221108

PDF( 1596 KB)

Study on Differential Expression Profile of lncRNA between Concurrent Chemoradioresistant Nasopharyngeal Carcinoma Cell Lines and Parental Cell Lines

doi: 10.12259/j.issn.2095-610X.S20221108

Qiuyan LIU^{1, 2},
Wei XIONG^{1
,
,},
Cheng LI¹

1.
Dept. of Radiation Therapy，Yunnan Cancer Hospital，The 3rd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650118
2.
Dept. of Oncology，Affiliated Hospital of Panzhihua University，Panzhihua Sichuan 617000，China

Received Date: 2022-05-06
Available Online: 2022-10-29
Publish Date: 2022-11-14

Abstract

Abstract

Objective To analyze the long non coding RNA （lncRNA） differentially expressed between nasopharyngeal carcinoma cell lines resistant to concurrent radiotherapy and chemotherapy and their parent cell lines, and explore the possible role of lncRNA in nasopharyngeal carcinoma resistance to concurrent radiotherapy and chemotherapy. Methods The concurrent chemoradiotherapy resistance model of nasopharyngeal carcinoma CEN1 and CNE2 cells was constructed in vitro, and the concurrent chemoradiotherapy resistance cell lines CEN1CRR and CNE2CRR of nasopharyngeal carcinoma cells were induced. The differentially expressed lncRNAs between the two groups of cell lines were screened by high-throughput sequencing, and the target genes of the differentially expressed lncRNAs were analyzed by GO and KEGG. Results With Log2FC > 1 or < - 1 and FDR < 0.05 as the differential expression criteria, 2746 lncRNAs （358 up-regulated and 2063 down-regulated） were differentially expressed in CNE1 CRR compared with CNE1. Compared with CNE2, CNE2CRR differentially expressed 3475 lncRNAs （265 up-regulated, 520 down-regulated）. In addition, 387 lncRNAs were downregulated in CNE1CRR and CNE2CRR, and 49 lncRNAs were upregulated in CNE1CRR and CNE2CRR. GO analysis showed that the target genes differentially expressing lncRNAs in the two groups of cells were involved in biological process （BP）, molecular function （MF）, and cell component （CC）. In the KEGG analysis results, it was found that in CNE1 group cells, the main signal pathways for the enrichment of target genes of differential lncRNAs were apoptosis, viral carcinogenesis, metabolic pathways, etc. In CNE2 group, the target genes of differential lncRNAs were mainly enriched through metabolic pathways （lipoic acid, pentose phosphate, arachidonic acid, ether lipid）, T cell receptor signaling pathways, nucleotide excision repair, etc. （P < 0.05）. Conclusions There are significant differences in lncRNA expression profiles between concurrent chemoradiotherapy-resistant cell lines and parental cell lines. These differentially expressed lncRNAs may participate in concurrent chemoradiotherapy resistance of nasopharyngeal carcinoma, and lay a foundation for its mechanism research.
- Long non-coding RNA（lncRNA）,
- Expression profile,
- Nasopharyngeal carcinoma cell,
- Concurrent chemoradioresistance,
- High-throughput sequencing

FullText(HTML)

References(19)

References

[1]	Gong L,Kwong D L,Dai W,et al. The stromal and immune landscape of nasopharyngeal carcinoma and its implications for precision medicine targeting the tumor microenvironment[J]. Frontiers in Oncology,2021,11:744889. doi: 10.3389/fonc.2021.744889
[2]	Chen Y P,Chan A,Le QT,et al. Nasopharyngeal carcinoma[J]. Lancet,2019,394(10192):64-80. doi: 10.1016/S0140-6736(19)30956-0
[3]	梁锌,杨剑,高婷,等. 中国鼻咽癌流行概况[J]. 中国肿瘤,2016,25(11):835-840. doi: 10.11735/j.issn.1004-0242.2016.11.A001
[4]	Chen Y P,Ismaila N,Chua M,et al. Chemotherapy in combination with radiotherapy for definitive-intent treatment of stage II-IVA nasopharyngeal carcinoma:CSCO and ASCO guideline[J]. J Clin Oncol,2021,39(7):840-859. doi: 10.1200/JCO.20.03237
[5]	Huang B,Li Y,Deng Z,et al. Regulatory mechanism of lncRNA CTBP1-AS2 in nasopharyngeal carcinoma cell proliferation and apoptosis via the miR-140-5p/BMP2 axis[J]. Protein Pept Lett,2022,29(7):621-630.
[6]	Cui M,Chang Y,Fang Q G,et al. Non-coding RNA Pvt1 promotes cancer stem cell-Like traits in nasopharyngeal cancer via inhibiting miR-1207[J]. Pathol Oncol Res,2019,25(4):1411-1422. doi: 10.1007/s12253-018-0453-1
[7]	Estrada-Meza C,Torres-Copado A,Loreti G L,et al. Recent insights into the microRNA and long non-coding RNA-mediated regulation of stem cell populations[J]. 3 Biotech,2022,12(10):270. doi: 10.1007/s13205-022-03343-8
[8]	Tang Y,He X. Long non-coding RNAs in nasopharyngeal carcinoma:biological functions and clinical applications[J]. Mol Cell Biochem,2021,476(9):3537-3550. doi: 10.1007/s11010-021-04176-4
[9]	Wang Y H,Guo Z,An L,et al. LINC-PINT impedes DNA repair and enhances radiotherapeutic response by targeting DNA-PKcs in nasopharyngeal cancer[J]. Cell Death Dis,2021,12(5):454. doi: 10.1038/s41419-021-03728-2
[10]	刘珊. 结直肠癌细胞同期放化疗抵抗关键调控基因的筛选[D]. 昆明: 昆明医科大学, 2015.
[11]	Bridges M C,Daulagala A C,Kourtidis A. LNCcation:lncRNA localization and function[J]. J Cell Biol,2021,220(2):e202009045.
[12]	Zheng J,Zhao Z,Ren H,et al. LncRNA HCG11 facilitates nasopharyngeal carcinoma progression through regulating miRNA-490-3p/MAP3K9 Axis[J]. Front Oncol,2022,12:872033. doi: 10.3389/fonc.2022.872033
[13]	Guo C,Ma X,He H,et al. Expression of ANCR in nasopharyngeal carcinoma patients and its clinical significance[J]. Medicine (Baltimore),2021,100(33):e26834.
[14]	Guo Z,Wang Y H,Xu H,et al. LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma[J]. Cell Death Dis,2021,12(1):69. doi: 10.1038/s41419-020-03302-2
[15]	Xiao J,He X. Involvement of non-coding RNAs in chemo- and radioresistance of nasopharyngeal carcinoma[J]. Cancer Manag Res,2021,13:8781-8794. doi: 10.2147/CMAR.S336265
[16]	Lee A,Tung S Y,Ng W T,et al. A multicenter,phase 3,randomized trial of concurrent chemoradiotherapy plus adjuvant chemotherapy versus radiotherapy alone in patients with regionally advanced nasopharyngeal carcinoma:10-year outcomes for efficacy and toxicity[J]. Cancer,2017,123(21):4147-4157. doi: 10.1002/cncr.30850
[17]	Li G,Liu Y,Liu C,et al. Genome-wide analyses of long noncoding RNA expression profiles correlated with radioresistance in nasopharyngeal carcinoma via next-generation deep sequencing[J]. BMC Cancer,2016,16:719. doi: 10.1186/s12885-016-2755-6
[18]	Niu Z,Wang F,Lv S,et al. HNRNPU-AS1 regulates cell proliferation and apoptosis via the microRNA 205-5p/AXIN2 axis and Wnt/beta-catenin signaling pathway in cervical cancer[J]. Mol Cell Biol,2021,41(10):e11521.
[19]	Zhang L,Zhao Y,Guan H,et al. HnRNPU-AS1 inhibits the proliferation,migration and invasion of HCC cells and induces autophagy through miR-556-3p/ miR-580-3p/SOCS6 axis[J]. Cancer Biomark,2022,34(3):443-457. doi: 10.3233/CBM-210261

Relative Articles(20)

Relative Articles

[1]	Feifei XIE, Yinzi XIN, Min XU, Jinghan LI, Wei WANG. Mechanisms of Long Non-Coding RNAs in Cartilage Development and Related Diseases. Journal of Kunming Medical University, 2024, 45(10): 1-7. doi: 10.12259/j.issn.2095-610X.S20241001
[2]	Jiang WU, Jiang ZHANG, Yanyan FEI, Qin LIU, Yuanjiao CHEN, Yuanxiao WANG, Shuifen DAI. Application of Knowledge-attitude-behavior Continuation in Nursing Education for Nasopharyngeal Cancer Patients After Radiotherapy and Chemotherapy. Journal of Kunming Medical University, 2024, 45(10): 166-172. doi: 10.12259/j.issn.2095-610X.S20241026
[3]	Xuedan WANG, Yifan LIU, Zihan YANG, Dandan ZHANG, Yi DUAN, Zhihui YANG. Analysis of the Clinicopathological and Molecular Features of Appendiceal Mucinous Neoplasms. Journal of Kunming Medical University, 2024, 45(7): 42-48. doi: 10.12259/j.issn.2095-610X.S20240706
[4]	Jiang WU, Shuifen DAI, Qin LIU, Shoutao YANG, Jiang ZHANG, Jiao YANG, Qiongyao GUAN. Effect of Nutrition Clinical Nursing Pathway for Nasopharyngeal Carcinoma Patients Concurrent Radiotherapy and Chemotherapy. Journal of Kunming Medical University, 2023, 44(5): 185-190. doi: 10.12259/j.issn.2095-610X.S20230510
[5]	Jianping HE, Mengxin LV, Maohua QIN, Lihong ZHU, Fengbo DANG, Yongbo SUN, Shengjun LUO, Lan LUO, Jian TANG. Gene Variation Analysis of 357 Cases with Gilbert Syndrome in Kunming. Journal of Kunming Medical University, 2023, 44(10): 155-160. doi: 10.12259/j.issn.2095-610X.S20231015
[6]	Jun GU, Zexi HE, Ting LUAN, Haifeng WANG, Jiansong WANG, Mingxia DING. Research Progress of Exosomes-derived Long Non-coding RNA in Bladder Cancer. Journal of Kunming Medical University, 2022, 43(1): 150-156. doi: 10.12259/j.issn.2095-610X.S20220110
[7]	Xiaoli WANG, Ting DING, Meiping JIANG, Kuixuan ZHU, Hongwei YANG. Optimal Total Nedaplatin Dose of Concurrent Chemotherapy in Patients with Stage III-IVA Nasopharyngeal Carcinoma. Journal of Kunming Medical University, 2022, 43(12): 66-74. doi: 10.12259/j.issn.2095-610X.S20221213
[8]	Fangyuan GUO, Wenliang LI, Fan XU, Xiangqun WEI. The Expression and Clinical Significance of Non-coding RNA in Cervical Cancer. Journal of Kunming Medical University, 2022, 43(5): 108-116. doi: 10.12259/j.issn.2095-610X.S20220527
[9]	Liju ZHANG, Xiaoming JIANG, Changxian CHEN, Xi WU, Zhenyong ZHANG, Weijun LIU. Long Non-coding RNA-p21 Regulates the MicroRNA-9 / Sirtuin-1 Signaling Pathway to Reverse Oxaliplatin Resistance in Colorectal Cancer Cells. Journal of Kunming Medical University, 2022, 43(5): 27-32. doi: 10.12259/j.issn.2095-610X.S20220519
[10]	Xinyi LI, Yuexiao LI, Lixian LI, Yongping LI, Ying GAO, Yanhong LI. Study on Microbiome of Supragingival Plaque of College Students with Gingivitis. Journal of Kunming Medical University, 2022, 43(2): 102-106. doi: 10.12259/j.issn.2095-610X.S20220207
[11]	Teng-fei ZHANG, Yue-feng HE, Lei ZHANG, Li ZHANG, Kai-yun YANG, Lian-qing LIU, Hui TAN. Expression of Long Non-coding RNA APTR，HEIH，FAS-ASA1，FAM83H-AS1，DICER1-AS1，PR-lncRNA in Lung Cancer. Journal of Kunming Medical University, 2021, 42(4): 49-52. doi: 10.12259/j.issn.2095-610X.S20210409
[12]	Wei Han Xiao , Zhang Ai Jun , Li Qiang , Jin Pei Sheng . . Journal of Kunming Medical University, 2020, 41(09): 50-55.
[13]	Hu Tao , Liu Guang Cai , Hong Ying , Chen Hong Lan , Chang Ye Fei , Jiang Shui , Tan Ya Xin , Xi Yang Yan Bin , Chen Bo . Effect of G6PD Knockdown on the Expression of miRNAs in Cervical Cancer Cells. Journal of Kunming Medical University, 2019, 40(03): 6-10.
[14]	Wu Jie , Zhang Yun Qiao , Teng Zhao Wei , You Xu , Liu Zi Jun , Ma Yu Han , Zhang Wen Yu , Feng Zi Qiao , Long Qing , Zeng Yong . . Journal of Kunming Medical University, 2019, 40(07): 137-141.
[15]	Wu Han Xin , Yu Jian Kun , Gao 淩, Li Ming Yang , Wu Xiao Hai , Tai Wen Lin . . Journal of Kunming Medical University, 2019, 40(01): 20-23.
[16]	Yin Shun Hui , Zhan Ye Ming , Jia Feng Mei , Ran Li Quan , Zhang Ming Zhu . . Journal of Kunming Medical University, 2019, 40(04): 23-30.
[17]	Xiong Jing Jing , Qi Wen Jin , Hu Hong Wei , Zeng Jie , Huang Rong , Ma Run Mei , Liu Mei , Huang Yong Kun . The Detection and Analysis of the Placenta Microbiota of Normal Term Delivery. Journal of Kunming Medical University, 2017, 38(11): 52-57.
[18]	Bai Hua . . Journal of Kunming Medical University,
[19]	Cai Yan . . Journal of Kunming Medical University,
[20]	. The Curative Effect of Cisplatin Concurrent Chemotherapy and Radiotherapy on Local Advanced Cervical Cancer. Journal of Kunming Medical University,