Effects of Puerarin on Th17/Treg Cell Immune Homeostasis and the Expression of Related Transcription Factors in Rats with Periodontitis

Le ZHAN; Ruiyu MA; Ni WAN; Liwen MO; Huiying CHEN; Li ZHANG

doi:10.12259/j.issn.2095-610X.S20221129

Volume 43 Issue 11

Nov. 2022

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Le ZHAN, Ruiyu MA, Ni WAN, Liwen MO, Huiying CHEN, Li ZHANG. Effects of Puerarin on Th17/Treg Cell Immune Homeostasis and the Expression of Related Transcription Factors in Rats with Periodontitis[J]. Journal of Kunming Medical University, 2022, 43(11): 36-43. doi: 10.12259/j.issn.2095-610X.S20221129

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Effects of Puerarin on Th17/Treg Cell Immune Homeostasis and the Expression of Related Transcription Factors in Rats with Periodontitis

doi: 10.12259/j.issn.2095-610X.S20221129

Le ZHAN¹,
Ruiyu MA²,
Ni WAN¹,
Liwen MO¹,
Huiying CHEN¹,
Li ZHANG^{1
,
,}

1.
School of Stomatology，Hainan Medical University， Haikou Hainan 571101
2.
School of Stomatology，Lanzhou University，Lanzhou Gansu 730000，China

Received Date: 2022-07-15
Available Online: 2022-10-29
Publish Date: 2022-11-14

Abstract

Abstract

Objective To study the effects of puerarin on the immune homeostasis of T helper 17 （Th17）/regulatory T （Treg） cells and the expression of related transcription factors in rats with periodontitis. Methods A rat periodontitis model was established using gingival isolation, silk ligature application and subgingival injection of Escherichia coli endotoxin （E-LPS）. The rats were randomly divided into a normal control group （group A）, a periodontitis model group （group B）, and a 200 mg/（kg-d） puerarin group （group C）. HE staining was used to observe the changes of periodontal tissue. Micro-ct correlated bone indexes were also analyzed to quantify the resorption of alveolar bone. The percentages of Th17 and Treg cells and the expression of interleukin （IL）-17, retinoic acid-related orphan receptor gamma t （RORγt）, IL-10 and forkhead transcription factor-3 （Foxp3）in periodontal tissues were monitored by flow Cytometry and Western blot （WB）, respectively. Results In the study, the roots of the teeth in the puerarin group were significantly proliferated with new osteoblasts. Flow cytometry showed that the proportion of TH17 and Treg cells in the puerarin group decreased, and the proportion of TH17/Treg cells also decreased . The protein expressions of IL-10 and Foxp3 were up-regulated in puerarin, while the histone expressions of IL-17 and RORγt were down-regulated in puerarin （P < 0.05）. Conclusions Puerarin can alleviate the periodontal tissue inflammatory response and alveolar bone resorption in rats with periodontitis and favor periodontal tissue healing by regulating Th17/Treg cell immune homeostasis and the expression of related transcription factors. The mechanism of action may be related to effective regulation of Th17/Treg cell immune homeostasis by puerarin.
- Puerarin,
- Periodontitis,
- Rats,
- Helper T cells,
- Regulatory T cells

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References(27)

References

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