Effect of VEGF-C on Radiation Sensitivity of Nasopharyngeal Carcinoma CNE-2 Cell Transplanted Mice

Xifang WU; Yong WANG; Lisha PENG; Jie LUO; Feng WANG

doi:10.12259/j.issn.2095-610X.S20221205

Volume 43 Issue 12

Dec. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(12): 6-11

Xifang WU, Yong WANG, Lisha PENG, Jie LUO, Feng WANG. Effect of VEGF-C on Radiation Sensitivity of Nasopharyngeal Carcinoma CNE-2 Cell Transplanted Mice[J]. Journal of Kunming Medical University, 2022, 43(12): 6-11. doi: 10.12259/j.issn.2095-610X.S20221205

Citation:

Xifang WU, Yong WANG, Lisha PENG, Jie LUO, Feng WANG. Effect of VEGF-C on Radiation Sensitivity of Nasopharyngeal Carcinoma CNE-2 Cell Transplanted Mice[J]. Journal of Kunming Medical University, 2022, 43(12): 6-11. doi: 10.12259/j.issn.2095-610X.S20221205

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Effect of VEGF-C on Radiation Sensitivity of Nasopharyngeal Carcinoma CNE-2 Cell Transplanted Mice

doi: 10.12259/j.issn.2095-610X.S20221205

1.
Dept. of Head and Neck Surgery，The 3rd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650118
2.
Dept. of Radiotherapy，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2021-12-06
Available Online: 2022-11-28
Publish Date: 2022-12-25

Abstract

Abstract

Objective To investigate the effect of interfering with the expression of VEGF-C on the radiosensitivity of the nasopharyngeal carcinoma CNE-2 cell transplanted tumor and related signaling pathways. Methods The mice with transplanted tumors of nasopharyngeal carcinoma CNE-2 were randomly divided into tumor body group, siRNA-NC group, siRNA-VEGF-C group; irradiation group; siRNA-NC+ irradiation group; siRNA-VEGF-C+irradiation group. Tumor growth was observed, and the protein expression of VEGF-C, ATM and P65 were detected by immunohistochemistry. Results Interference of VEGF-C expression in nasopharyngeal carcinoma CNE-2 cell transplanted tumors showed significantly slower tumor growth after irradiation. Immunohistochemical test results showed that after tumor transplantation of mice interfered with VEGF-C, VEGF-C and ATM were significantly decreased （P < 0.05）, while P65 was increased （P < 0.05）. After combined irradiation, VEGF-C and ATM were significantly decreased （P < 0.05）. Conclusion In mouse nasopharyngeal carcinoma CNE-2 cell transplantation tumors, interfering with VEGF-C gene affects downstream ATM by activating NF-kB signaling pathway, thereby increasing the sensitivity of radiotherapy.
- Nasopharyngeal carcinoma,
- VEGF-C,
- Radiation sensitivity,
- Experimental animals

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References(15)

References

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