Expression Levels of TNF-α，sTNFR-55 and sTNFR-75 in Induced Sputum and Serum of AECOPD Patients and Their Clinical Significance

Qingsong MA; Zhihuan ZHAO; Lirong MA; Weijing LI; Ling LIU

doi:10.12259/j.issn.2095-610X.S20230112

Volume 44 Issue 1

Jan. 2023

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Qingsong MA, Zhihuan ZHAO, Lirong MA, Weijing LI, Ling LIU. Expression Levels of TNF-α，sTNFR-55 and sTNFR-75 in Induced Sputum and Serum of AECOPD Patients and Their Clinical Significance[J]. Journal of Kunming Medical University, 2023, 44(1): 41-46. doi: 10.12259/j.issn.2095-610X.S20230112

Citation:

Qingsong MA, Zhihuan ZHAO, Lirong MA, Weijing LI, Ling LIU. Expression Levels of TNF-α，sTNFR-55 and sTNFR-75 in Induced Sputum and Serum of AECOPD Patients and Their Clinical Significance[J]. Journal of Kunming Medical University, 2023, 44(1): 41-46. doi: 10.12259/j.issn.2095-610X.S20230112

Citation:

Qingsong MA, Zhihuan ZHAO, Lirong MA, Weijing LI, Ling LIU. Expression Levels of TNF-α，sTNFR-55 and sTNFR-75 in Induced Sputum and Serum of AECOPD Patients and Their Clinical Significance[J]. Journal of Kunming Medical University, 2023, 44(1): 41-46. doi: 10.12259/j.issn.2095-610X.S20230112

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Expression Levels of TNF-α，sTNFR-55 and sTNFR-75 in Induced Sputum and Serum of AECOPD Patients and Their Clinical Significance

doi: 10.12259/j.issn.2095-610X.S20230112

Dept. of Respiratory Critical Care Medicine，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2022-11-14
Available Online: 2022-12-23
Publish Date: 2023-01-18

Abstract

Abstract

Objective To investigate the different expression levels of TNF-α, sTNFR-55 and sTNFR-75 in the induced sputum and serum of AECOPD patients and their clinical significance. Methods Forty AECOPD patients were enrolled and hospitalized during January 2019 and December 2020. All subjects received the conventional therapy. The expression levels of TNF-α, sTNFR-55 and sTNFR-75 in the induced sputum and serum before the treatment （group Ⅰ） and after the treatment （group Ⅱ） were compared and their correlation with the severity of the disease was analyzed. Meanwhile, the correlation between three inflammatory mediators in the induced sputum and serum was analyzed. According to the smoking history, they were divided into smoking cessation group （group Ⅲ） and smoking group （group Ⅳ）. The changes of TNF-α, sTNFR-55 and sTNFR-75 levels after quitting smoking were also compared and analyzed. Results （1） After the treatment, the levels of TNF-α in sputum and serum decreased significantly （P < 0.05）, while the levels of sTNFR-55 in sputum increased （t = 3.044, P = 0.004）, and the levels of sTNFR-55 and sTNFR-75 in serum showed no significant difference （P > 0.05）. （2） FEV₁, FEV₁%pred, FEV₁/FVC were negatively correlated with the level of TNF-α in sputum （r was -0.827, -0.865, -0.868, respectively, P < 0.001）, and positively correlated with the level of sTNFR in sputum （r_sTNFR-55 was 0.415, 0.330 and 0.305, respectively, P < 0.05; r_sTNFR-75 was 0.903, 0.969 and 0.965, respectively, P < 0.001）. （3） APACHEIII score was positively correlated with the level of TNF-α in sputum （r = 0.374, P = 0.001）, and negatively correlated with the level of sTNFR level （r was -0.227, -0.341, respectively, P < 0.05）. （4） There was no correlation between TNF-α/sTNFR levels in the induced sputum and serum （P > 0.05）, and its level in serum had no correlation with lung function index and APACHEII score （P > 0.05）. （5） Compared with the smoking group, the TNF-α level in the induced sputum of the smoking cessation group was lower （Z = -2.626, P = 0.008）, while the sTNFR-55 and sTNFR-75 levels in the induced sputum were higher （t = 2.716, P = 0.01; Z = -2.696, P = 0.006）. Conclusion The levels of TNF-α, sTNFR-55 and sTNFR-75 in the induced sputum can be used to evaluate the efficacy and severity of AECOPD patients. No correlation has been found between systemic and airway inflammation in AECOPD patients, and airway inflammation may still progress after smoking cessation.
- Chronic obstructive pulmonary disease,
- Tumor necrosis factor-α,
- Soluble tumor necrosis factor receptor,
- Induced sputum

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