Analysis of Serum High-sensitivity HBV DNA in Patients with Chronic Hepatitis B with Low Viral Load in Kunming Area

Dong PU; Tingting YU; Lihua LI; Runwu ZHANG; Kunli WU; Dongling LI

doi:10.12259/j.issn.2095-610X.S20230114

Volume 44 Issue 1

Jan. 2023

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Article Navigation > Journal of Kunming Medical University > 2023 > 44(1): 116-121

Dong PU, Tingting YU, Lihua LI, Runwu ZHANG, Kunli WU, Dongling LI. Analysis of Serum High-sensitivity HBV DNA in Patients with Chronic Hepatitis B with Low Viral Load in Kunming Area[J]. Journal of Kunming Medical University, 2023, 44(1): 116-121. doi: 10.12259/j.issn.2095-610X.S20230114

Citation:

Dong PU, Tingting YU, Lihua LI, Runwu ZHANG, Kunli WU, Dongling LI. Analysis of Serum High-sensitivity HBV DNA in Patients with Chronic Hepatitis B with Low Viral Load in Kunming Area[J]. Journal of Kunming Medical University, 2023, 44(1): 116-121. doi: 10.12259/j.issn.2095-610X.S20230114

Citation:

Dong PU, Tingting YU, Lihua LI, Runwu ZHANG, Kunli WU, Dongling LI. Analysis of Serum High-sensitivity HBV DNA in Patients with Chronic Hepatitis B with Low Viral Load in Kunming Area[J]. Journal of Kunming Medical University, 2023, 44(1): 116-121. doi: 10.12259/j.issn.2095-610X.S20230114

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Analysis of Serum High-sensitivity HBV DNA in Patients with Chronic Hepatitis B with Low Viral Load in Kunming Area

doi: 10.12259/j.issn.2095-610X.S20230114

The Clinical Laboratory，The 3rd People’s Hospital of Kunming/Clinical Center for Diseases in Yunnan Province，Kunming Yunnan 650041，China

Received Date: 2022-10-23
Available Online: 2022-12-08
Publish Date: 2023-01-18

Abstract

Abstract

Objective To investigate the relationship among high-sensitive HBV-DNA load , high-sensitivity HBV-DNA load, HBV genotype distribution, HBV P region drug resistance mutation, HBeAg, HBsAg, ALT and its value of clinical application in patients among the chronic hepatitis B with persistent low-level viremia in Kunming. Methods A total of 707 patients with chronic hepatitis B who received inpatient and outpatient treatment in the Third People’s Hospital of Kunming from January 2021 to December 2021 were selected. The relationship between different HBV-DNA loads and HBeAg, HBsAg and ALT was analyzed. Results Among the 707 chronic hepatitis B patients with persistent low load in the study, 446 cases had high-sensitivity HBV DNA load < 500 IU/ml, accounting for （63.50%）, and there were 576 chronic hepatitis B patients with high-sensitivity HBV DNA < 2000 IU/ml, accounting for （81.90%）; 262 cases （37.05%） were HBeAg positive; genotype C accounted for the highest proportion of 60.53% （428/707）. Among the 196 chronic hepatitis B patients with HBeAg （+） and HBV DNA < 2000 IU/mL, males were dominant, accounting for 69.89%; ALT > 40 U/L accounted for 28.57% and 73.47% of patients had HBV DNA > 20 IU/mL mL. Among the 265 chronic hepatitis B patients with HBV DNA < 20 IU/mL, HBeAg （-） accounted for 80.38% （213 cases）, and HBeAg （+） accounted for 19.62% （52 cases）. Compared with the clinical indicators, the positive rate of HBeAg, the abnormal rate of ALT, the mutation rate of drug resistance sites and other sites were significantly different among different HBV DNA load levels （P < 0.05）. Conclusion For chronic hepatitis B patients with persistent low-level viremia, the use of high sensitivity PCR technology to regularly monitor HBV DNA of hepatitis B virus in combination with quantitative monitoring of clinical indicators such as HBeAg, HBsAg, ALT, etc. can provide the objective reference for the clinical treatment of follow-up patients.
- Hepatitis B virus,
- Low viral load,
- High-sensitivity HBV DNA,
- Gene mutation,
- HbeAg

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