Mutation Analysis of <i>OprD</i> Gene in Pseudomonas Aeruginosa with Car-R/ Ceph-S

Xi ZHENG; Jinzhu MA; Qinchun LI; Yu GAO; Ying PAN; Guibo SONG

doi:10.12259/j.issn.2095-610X.S20230121

Volume 44 Issue 1

Jan. 2023

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Xi ZHENG, Jinzhu MA, Qinchun LI, Yu GAO, Ying PAN, Guibo SONG. Mutation Analysis of OprD Gene in Pseudomonas Aeruginosa with Car-R/ Ceph-S[J]. Journal of Kunming Medical University, 2023, 44(1): 7-11. doi: 10.12259/j.issn.2095-610X.S20230121

Citation:

Xi ZHENG, Jinzhu MA, Qinchun LI, Yu GAO, Ying PAN, Guibo SONG. Mutation Analysis of OprD Gene in Pseudomonas Aeruginosa with Car-R/ Ceph-S[J]. Journal of Kunming Medical University, 2023, 44(1): 7-11. doi: 10.12259/j.issn.2095-610X.S20230121

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Mutation Analysis of OprD Gene in Pseudomonas Aeruginosa with Car-R/ Ceph-S

doi: 10.12259/j.issn.2095-610X.S20230121

1.
The Clinical Laboratory，The 2nd Affiliated Hospital of Yunnan University of Traditional Chinese Medicine，KunmingYunnan 650041
2.
Dep. of Medical Laboratory，The 1st Affiliated Hospital of Kunming Medical University，Yunnan Key Laboratory of Laboratory Medicine，Yunnan Medical Laboratory Clinical Medical Research Center，Kunming Yunnan 650032，China

Received Date: 2022-10-15
Available Online: 2022-12-23
Publish Date: 2023-01-18

Abstract

Abstract

Objective To study the mutation types of OprD gene in Pseudomonas aeruginosa resistant to carbapenems but sensitive to cephalosporins （Car-R/Ceph-S）. Methods Ten strains of Pseudomonas aeruginosa which were resistant to carbapenems but sensitive to cephalosporins were isolated and cultured in the First Affiliated Hospital of Kunming Medical University from January 2021 to March 2021. Polymerase chain reaction （PCR） was used to detect the carrying status of OprD gene in Pseudomonas aeruginosa, and the mutation type of OprD gene was analyzed by sequencing. Results All the 10 strains showed obvious OprD bands and the sequencing analysis showed that there were 2 frameshift mutations, 8 amino acid substitutions, 2 early termination codons and 4 large fragment deletions. Conclusion The mutation types of OprD gene in carbapenem-resistant but cephalosporin-sensitive （Car-R/Ceph-S） strains of Pseudomonas aeruginosa are diversified with the highest frequency of point mutations, deletion of large segments and early termination of codons. These mutations lead to changes in the structure of OprD, resulting in the occurrence of drug resistance.
- Pseudomonas aeruginosa,
- Carbapenem resistance,
- Cephalosporin sensitivity,
- OprD,
- Gene mutation,
- Sequencing

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References(14)

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