Pyrotosis Participates in the Pathogenesis of Necrotizing Enterocolitis in Early Offspring Rats

Jing WANG; Hongying MI; Yi ZHANG; Li LI; Jianhua YU; Liqiao LIU; Qingyu LIU; Liwei WANG

doi:10.12259/j.issn.2095-610X.S20230124

Volume 44 Issue 1

Jan. 2023

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Article Navigation > Journal of Kunming Medical University > 2023 > 44(1): 25-30

Jing WANG, Hongying MI, Yi ZHANG, Li LI, Jianhua YU, Liqiao LIU, Qingyu LIU, Liwei WANG. Pyrotosis Participates in the Pathogenesis of Necrotizing Enterocolitis in Early Offspring Rats[J]. Journal of Kunming Medical University, 2023, 44(1): 25-30. doi: 10.12259/j.issn.2095-610X.S20230124

Citation:

Jing WANG, Hongying MI, Yi ZHANG, Li LI, Jianhua YU, Liqiao LIU, Qingyu LIU, Liwei WANG. Pyrotosis Participates in the Pathogenesis of Necrotizing Enterocolitis in Early Offspring Rats[J]. Journal of Kunming Medical University, 2023, 44(1): 25-30. doi: 10.12259/j.issn.2095-610X.S20230124

Citation:

Jing WANG, Hongying MI, Yi ZHANG, Li LI, Jianhua YU, Liqiao LIU, Qingyu LIU, Liwei WANG. Pyrotosis Participates in the Pathogenesis of Necrotizing Enterocolitis in Early Offspring Rats[J]. Journal of Kunming Medical University, 2023, 44(1): 25-30. doi: 10.12259/j.issn.2095-610X.S20230124

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Pyrotosis Participates in the Pathogenesis of Necrotizing Enterocolitis in Early Offspring Rats

doi: 10.12259/j.issn.2095-610X.S20230124

1.
School of Clinical Medicine，Dali University，Dali Yunnan 671003
2.
Dept. of Pediatrics，The 1st People’s Hospital of Yunnan Province，Kunming Yunnan 650032
3.
School of Food Science and Engineering，Kunming University of Science and Technology，Kunming Yunnan 650032
4.
Clinical Medical Research Center，The 1st People’s Hospital of Yunnan Province，Kunming Yunnan 650032
5.
School of Clinical Medicine，Kunming University of Science and Technology，Kunming Yunnan 650032，China

Received Date: 2022-10-15
Available Online: 2022-12-24
Publish Date: 2023-01-18

Abstract

Abstract

Objective To establish a mouse model of necrotizing enterocolitis and detect the key protein molecules in serum of neonatal rats with early NEC, so as to preliminatively determine whether necrotizing enterocolitis is involved in early neonatal necrotizing enterocolitis. Methods The NEC mouse model was established and randomly divided into the experimental group and control group with 12 newborn rats in each group. The neonatal rats in the experimental group were induced into NEC animal models. Blood samples were extracted and the concentrations of IL-18, Gasdermin D and Caspase-1 in serum of all rats were detected by Elisa kit. The concentration of target protein in serum of the two groups was compared, α = 0.05. Results The concentrations of Gasdermin D and Caspase-1 in serum of rats in the two groups were significantly different, with P values of 0.017 and < 0.001, respectively. It could be considered that the concentrations of Gasdermin D and caspase-1 in serum of rats in the experimental group were higher than those in the control group. There was no significant difference in serum IL-18 concentration between the two groups （P = 0.755）. Conclusion Gasdermin D mediated pyroptosis may be involved in the pathogenesis of early NEC.
- Gasdermin D,
- Pyroptosis,
- Necrotizing enterocolitis,
- Neonatal

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References(32)

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