Establishment and Significance of Screening System for Acute Leukemia in Children Based on Detection of TEL-AML1 Fusion Gene in Neonatal Cord Blood

Yaling ZHAO; Kun WU; Rong HUANG; Genya HE; Zhenbo DING; Caiying ZHANG

doi:10.12259/j.issn.2095-610X.S20230126

Volume 44 Issue 1

Jan. 2023

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Article Navigation > Journal of Kunming Medical University > 2023 > 44(1): 54-58

Yaling ZHAO, Kun WU, Rong HUANG, Genya HE, Zhenbo DING, Caiying ZHANG. Establishment and Significance of Screening System for Acute Leukemia in Children Based on Detection of TEL-AML1 Fusion Gene in Neonatal Cord Blood[J]. Journal of Kunming Medical University, 2023, 44(1): 54-58. doi: 10.12259/j.issn.2095-610X.S20230126

Citation:

Yaling ZHAO, Kun WU, Rong HUANG, Genya HE, Zhenbo DING, Caiying ZHANG. Establishment and Significance of Screening System for Acute Leukemia in Children Based on Detection of TEL-AML1 Fusion Gene in Neonatal Cord Blood[J]. Journal of Kunming Medical University, 2023, 44(1): 54-58. doi: 10.12259/j.issn.2095-610X.S20230126

Citation:

Yaling ZHAO, Kun WU, Rong HUANG, Genya HE, Zhenbo DING, Caiying ZHANG. Establishment and Significance of Screening System for Acute Leukemia in Children Based on Detection of TEL-AML1 Fusion Gene in Neonatal Cord Blood[J]. Journal of Kunming Medical University, 2023, 44(1): 54-58. doi: 10.12259/j.issn.2095-610X.S20230126

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Establishment and Significance of Screening System for Acute Leukemia in Children Based on Detection of TEL-AML1 Fusion Gene in Neonatal Cord Blood

doi: 10.12259/j.issn.2095-610X.S20230126

1.
Dept.of Pediatrics
2.
Dept. of Medical Laboratory
3.
Dept. of Obstetrics，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2022-10-16
Available Online: 2022-12-24
Publish Date: 2023-01-18

Abstract

Abstract

Objective To establish an early screening system for childhood acute lymphoblastic leukemia based on the detection of TEL-AML1 fusion gene in neonatal umbilical cord blood and to explore its clinical significance. Methods First, the newborn with high risk factors of childhood acute leukemia was screened through questionnaire survey. Then, the umbilical cord blood of high-risk neonates at birth was collected and the mRNA of umbilical cord blood mononuclear cells was extracted within 12 to 24 hours. The TEL-AML1 fusion gene in umbilical cord blood was detected by RT-PCR, so as to build a screening system and analyze the feasibility, efficiency and practicality of the screening system. Results 231 cases of high-risk population were screened, including 118 males （51.1%） and 113 females （48.9%）. The TEL-AML1 fusion gene was negative by RT-PCR. The average time spent was （12.3±0.6） hours. The average cost was 200RMB and the satisfaction rate and acceptance rate of patient were both 100%. Conclusion The early screening system for childhood acute lymphoblastic leukemia based on the detection of TEL-AML1 fusion gene in neonatal umbilical cord blood is scientific in theory and feasible in clinical practice, which provides a basis for early screening of childhood acute lymphoblastic leukemia in clinical practice.
- Umbilical cord blood,
- Child,
- Acute leukemia,
- TEL-AML1,
- Fusion gene

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References(17)

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