Possible Roles of MiR-219-5p Targeting SOX5 in the Development of Oral Cancer

Ting ZHOU; Ying HE; Xiaohan DONG; Yanbin XIYANG; Bo CHEN; Jun TONG; Rui MAO

doi:10.12259/j.issn.2095-610X.S20230201

Volume 44 Issue 2

Feb. 2023

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Ting ZHOU, Ying HE, Xiaohan DONG, Yanbin XIYANG, Bo CHEN, Jun TONG, Rui MAO. Possible Roles of MiR-219-5p Targeting SOX5 in the Development of Oral Cancer[J]. Journal of Kunming Medical University, 2023, 44(2): 45-51. doi: 10.12259/j.issn.2095-610X.S20230201

Citation:

Ting ZHOU, Ying HE, Xiaohan DONG, Yanbin XIYANG, Bo CHEN, Jun TONG, Rui MAO. Possible Roles of MiR-219-5p Targeting SOX5 in the Development of Oral Cancer[J]. Journal of Kunming Medical University, 2023, 44(2): 45-51. doi: 10.12259/j.issn.2095-610X.S20230201

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Possible Roles of MiR-219-5p Targeting SOX5 in the Development of Oral Cancer

doi: 10.12259/j.issn.2095-610X.S20230201

Ting ZHOU¹,
Ying HE^{1, 2},
Xiaohan DONG³,
Yanbin XIYANG³,
Bo CHEN⁴,
Jun TONG⁵,
Rui MAO^{1
,
,}

1.
School of Stomatology，Kunming Medical University，Kunming Yunnan 650500
2.
Outpatient Department，Yunnan Province Corps Hospital，Chinese People’s Armed Police Forces，Kunming Yunnan 650100
3.
Institute of Neuroscience，Basic Medical College
4.
Science and Technology Achievement Incubation Center
5.
Physical Education Department，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2022-12-08
Available Online: 2023-01-31
Publish Date: 2023-02-25

Abstract

Abstract

Objectives To explore whether the expressions of miR-219-5p and / or SRY-related high-mobility-group box 5 （SOX5） in oral squamous cell carcinoma cells are differ from normal cells. We also aim to confirm the transcription regulation in SOX5 by miR-219-5p. Methods Human oral squamous cell lines SCC4 and SCC9 were employed. The expression levels of miR-219-5p and SOX5 were detected in cancer cells （indicated by RT-qPCR and Western blot）. Genetic analysis and luciferase assay were employed to verify whether miR-219-5p targets SOX5 by binding to the specific region of 3'-UTR. Results Our data showed that high miR-219-5p and low SOX5 levels were detected in vitro SCC4 and SCC9 cells （P < 0.05）. miR-219-5p was negatively correlated with SOX5 expression. Genetic analysis and luciferase assay revealed that miR-291-5p regulated SOX5 expression by targeting the 3'untranslated region （3'-UTR） of the SOX5 mRNA. Conclusions This study demonstrates that miR-219-5p is involved in the development and prognosis of oral cancer by targeting the expression of SOX5, and can be a novel candidate for clinical prognosis and/or therapy.
- Oral cancer cells,
- miR-219-5p,
- SOX5,
- Target regulation

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References(19)

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