Study on Osteogenic Ability of Dental Pulp Stem Cells at Different Concentrations

Shibing QIAN; Lingpeng ZHANG; Lingyun YIN; Changquan LI; Hu LI; Hongbin YU

doi:10.12259/j.issn.2095-610X.S20230214

Volume 44 Issue 2

Feb. 2023

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2023 > 44(2): 61-68

Shibing QIAN, Lingpeng ZHANG, Lingyun YIN, Changquan LI, Hu LI, Hongbin YU. Study on Osteogenic Ability of Dental Pulp Stem Cells at Different Concentrations[J]. Journal of Kunming Medical University, 2023, 44(2): 61-68. doi: 10.12259/j.issn.2095-610X.S20230214

Citation:

Shibing QIAN, Lingpeng ZHANG, Lingyun YIN, Changquan LI, Hu LI, Hongbin YU. Study on Osteogenic Ability of Dental Pulp Stem Cells at Different Concentrations[J]. Journal of Kunming Medical University, 2023, 44(2): 61-68. doi: 10.12259/j.issn.2095-610X.S20230214

Citation:

PDF( 3343 KB)

Study on Osteogenic Ability of Dental Pulp Stem Cells at Different Concentrations

doi: 10.12259/j.issn.2095-610X.S20230214

Dept. of Stomatology，Yan’an Hospital，Kunming City，The Affiliated Yan’an Hospital，Kunming Medical University，Kunming Yunnan 650051，China

Received Date: 2022-12-08
Publish Date: 2023-02-25

Abstract

Abstract

Objective To explore the optimal osteogenic ratio of Dental pulp stem cells （DPSC） with different concentrations in quantitative Bio-Oss bone powder. Methods The primary cells were isolated and cultured by adherent method. The surface markers of DPSC were identified by flow cytometry, and the multidirectional differentiation potential of DPSC was identified by osteogenesis and adipogenesis. DPSC was transfected with GFP lentivirus, and GFP-DPSC was screened with puromycin. The cells in groups 1×10⁵, 2×10⁵, 4×10⁵ and 8×10⁵ were inoculated with 0.05 g bone powder and cultured for osteogenesis, while the control group did not add bone powder. Alkaline phosphatase activity was measured on day 3 and day 7. On the 7th day of culture, the morphological changes of the cells in bone powder were observed under scanning electron microscope. Results The DPSC was derived from mesenchymal stem cells with high expression of related surface markers and could differentiate into adipogenic and osteogenic cells. GFP was successfully transfected into DPSC, and alkaline phosphatase activity was detected on the 3rd and 7th day, and the experimental group was higher than the control group, and the difference was statistically significant （P < 0.05）. Scanning electron microscopy showed that DPSC crawled on the surface of bone powder, and some cells extended pseudopodia, and the cells of 4×10 ⁵ and 8×10⁵ groups were more dense. Conclusions The combined culture of 4×10⁵-8×10⁵ DPSC and 0.05 g Bio-Oss bone powder has a good osteogenic effect, which can shorten the osteogenic cycle. If the cells are inoculated too much, the osteogenic effect is inhibited, resulting in the waste of stem cells.
- Bio-Oss bone meal,
- Pulp stem cells,
- Osteogenic differentiation,
- Scanning electron microscopy

FullText(HTML)

References(25)

References

[1]	Le Thieu M K,Homayouni A,Haeren L R,et al. Impact of simultaneous placement of implant and block bone graft substitute: an in vivo peri-implant defect model[J]. Biomater Res,2021,25(1):43-52. doi: 10.1186/s40824-021-00245-3
[2]	Shiu S T,Lee W F,Chen S M,et al. Effect of different bone grafting materials and mesenchymal stem cells on bone regeneration: A micro-computed tomography and histomorphometric study in a rabbit calvarial defect model[J]. Int J Mol Sci,2021,22(15):8101-8115. doi: 10.3390/ijms22158101
[3]	Shi X,Mao J,Liu Y. Pulp stem cells derived from human permanent and deciduous teeth: Biological characteristics and therapeutic applications[J]. Stem Cells Transl Med,2020,9(4):445-464. doi: 10.1002/sctm.19-0398
[4]	Tsutsui T W. Dental pulp stem cells: advances to applications[J]. Stem Cells Cloning,2020,13:33-42.
[5]	Gendviliene I,Simoliunas E,Alksne M,et al. Effect of extracellular matrix and dental pulp stem cells on bone regeneration with 3D printed PLA/HA composite scaffolds[J]. Eur Cell Mater,2021,41:204-215. doi: 10.22203/eCM.v041a15
[6]	Tanikawa D Y S,Pinheiro C C G,Almeida M C A,et al. Deciduous Dental Pulp Stem Cells for Maxillary Alveolar Reconstruction in Cleft Lip and Palate Patients[J]. Stem Cells Int,2020,2020:6234167.
[7]	Gil L F,Nayak V V,Benalcazar Jalkh E B,et al. Laddec(R) versus Bio-Oss(R): The effect on the healing of critical-sized defect - Calvaria rabbit model[J]. J Biomed Mater Res B Appl Biomater,2022,110(12):2744-2750. doi: 10.1002/jbm.b.35125
[8]	Li Y,Zhou W,Li P,et al. Comparison of the osteogenic effectiveness of an autogenous demineralised dentin matrix and Bio-Oss(R) in bone augmentation: a systematic review and meta-analysis[J]. Br J Oral Maxillofac Surg,2022,60(7):868-876. doi: 10.1016/j.bjoms.2022.03.009
[9]	Dai Y,Xu J,Han X H,et al. Clinical efficacy of mineralized collagen (MC) versus anorganic bovine bone (Bio-Oss) for immediate implant placement in esthetic area: a single-center retrospective study[J]. BMC Oral Health,2021,21(1):390-398. doi: 10.1186/s12903-021-01752-4
[10]	Shamsoddin E,Houshmand B,Golabgiran M. Biomaterial selection for bone augmentation in implant dentistry: A systematic review[J]. J Adv Pharm Technol Res,2019,10(2):46-50. doi: 10.4103/japtr.JAPTR_327_18
[11]	RAPONE B,INCHINGOLO A D,TRASARTI S,et al. Long-Term Outcomes of Implants Placed in Maxillary Sinus Floor Augmentation with Porous Fluorohydroxyapatite (Algipore((R)) FRIOS((R))) in Comparison with Anorganic Bovine Bone (Bio-Oss((R))) and Platelet Rich Plasma (PRP): A Retrospective Study[J]. J Clin Med,2022,11(9):2491-2503. doi: 10.3390/jcm11092491
[12]	Iaquinta M R,Martini F,D'agostino A,et al. Stem cell fate and immunomodulation promote bone regeneration via composite bio-oss((R))/avitene(TM) biomaterial[J]. Front Bioeng Biotechnol,2022,10:873814. doi: 10.3389/fbioe.2022.873814
[13]	Kosinski M,Figiel-dabrowska A,Lech W,et al. Bone defect repair using a bone substitute supported by mesenchymal stem cells derived from the umbilical cord[J]. Stem Cells Int,2020,2020:1321283.
[14]	Jiang Y H,Shang Y,Zou D H,et al. [Effect of rat allogeneic BMSCs-Bio-Oss-bFGF compound on tooth extraction healing: a micro-CT study][J]. Shanghai Kou Qiang Yi Xue,2022,31(1):38-43.
[15]	Zhou Q,Yu B H,Liu W C,et al. BM-MSCs and bio-oss complexes enhanced new bone formation during maxillary sinus floor augmentation by promoting differentiation of BM-MSCs[J]. In Vitro Cell Dev Biol Anim,2016,52(7):757-771. doi: 10.1007/s11626-015-9995-7
[16]	Xu X,Fang K,Wang L,et al. Local application of semaphorin 3a combined with adipose-derived stem cell sheet and anorganic bovine bone granules enhances bone regeneration in type 2 diabetes mellitus rats[J]. Stem Cells Int,2019,2019:2506463.
[17]	Chen Y,Huang H,Li G,et al. Dental-derived mesenchymal stem cell sheets: a prospective tissue engineering for regenerative medicine[J]. Stem Cell Res Ther,2022,13(1):38-52. doi: 10.1186/s13287-022-02716-3
[18]	Alksne M,Kalvaityte M,Simoliunas E,et al. Dental pulp stem cell-derived extracellular matrix: autologous tool boosting bone regeneration[J]. Cytotherapy,2022,24(6):597-607. doi: 10.1016/j.jcyt.2022.02.002
[19]	Davies O G,Cooper P R,Shelton R M,et al. A comparison of the in vitro mineralisation and dentinogenic potential of mesenchymal stem cells derived from adipose tissue,bone marrow and dental pulp[J]. J Bone Miner Metab,2015,33(4):371-382. doi: 10.1007/s00774-014-0601-y
[20]	Lee Y C,Chan Y H,Hsieh S C,et al. Comparing the osteogenic potentials and bone regeneration capacities of bone marrow and dental pulp mesenchymal stem cells in a rabbit calvarial bone defect model[J]. Int J Mol Sci,2019,20(20):5015-5024. doi: 10.3390/ijms20205015
[21]	Lorusso F,Inchingolo F,Dipalma G,et al. Synthetic scaffold/dental pulp stem cell (DPSC) tissue engineering constructs for bone defect treatment: an animal studies literature review[J]. Int J Mol Sci,2020,21(24):9765-9784. doi: 10.3390/ijms21249765
[22]	Wang F,Li Q,Wang Z. A comparative study of the effect of Bio-Oss((R)) in combination with concentrated growth factors or bone marrow-derived mesenchymal stem cells in canine sinus grafting[J]. J Oral Pathol Med,2017,46(7):528-536. doi: 10.1111/jop.12507
[23]	Qin R,Cui Z,Zhou H,et al. Effect of lentivirus-mediated BMP2 from autologous tooth on the proliferative and osteogenic capacity of human periodontal ligament cells[J]. J Periodontal Res,2022,57(4):869-879. doi: 10.1111/jre.13025
[24]	Zhou C,Zhang D,Zou J,et al. Substrate Compliance Directs the Osteogenic Lineages of Stem Cells from the Human Apical Papilla via the Processes of Mechanosensing and Mechanotransduction[J]. ACS Appl Mater Interfaces,2019,11(29):26448-26459. doi: 10.1021/acsami.9b07147
[25]	Shahabipour F,Oskuee R K,Shokrgozar M A,et al. CRISPR/Cas9 mediated GFP-human dentin matrix protein 1 (DMP1) promoter knock-in at the ROSA26 locus in mesenchymal stem cell for monitoring osteoblast differentiation[J]. J Gene Med,2020,22(12):e3288.

Relative Articles(20)

Relative Articles

[1]	Long PU, Xuanran ZHOU, Chenrong JIANG, Yunxuan LI, Yong YUAN. Effect of Inhibiting PPARγ Expression on Osteogenic Differentiation of BMSCs. Journal of Kunming Medical University, 2024, 45(9): 17-23. doi: 10.12259/j.issn.2095-610X.S20240903
[2]	Shibing QIAN, Huiping SHI, Yanqiu LI, Rongyu YANG, Kaiwen DUAN. Research Progress on Osteogenic Differentiation of Apical Papilla Stem Cells. Journal of Kunming Medical University, 2024, 45(9): 168-173. doi: 10.12259/j.issn.2095-610X.S20240926
[3]	Rufang LI, Qiuyan LI, Xia CUI, Xia ZHAO, Limei WANG, hai LAN. Remineralization Effect of Caesalpinia Sappan L. on Artificial Demineralization of Enamel Caries. Journal of Kunming Medical University, 2023, 44(2): 23-26. doi: 10.12259/j.issn.2095-610X.S20230208
[4]	Qian XU, Yumei CUI, Siming MA, Yunhong LIN, Yijing XIONG, Zijun SONG, Xudong LI. miR-148a-3p Targeting SMURF2 in Regulating Osteogenic Differentiation and Enamel Development during In Vitro Tooth Organogenesis. Journal of Kunming Medical University, 2023, 44(11): 16-21. doi: 10.12259/j.issn.2095-610X.S20231103
[5]	Xiaobing TAN, Min ZHANG, Yu GUO, Linling DU, Qingyuan DAI. Effect of miR-130b-3p on the Reprogramming of Human Dental Origin iPSCs. Journal of Kunming Medical University, 2022, 43(12): 18-22. doi: 10.12259/j.issn.2095-610X.S20221204
[6]	Jing ZHOU, Ping XIONG, Chao-feng LIU, Li-qiong CHEN, Yong-hui ZHANG, Ke SHI, Yan NIE, Yan LIU. Periodontal Ligament Stem Cell BMP-2-PSH Composite Membrane in Repairing Alveolar Bone Defect in New Zealand Rabbits. Journal of Kunming Medical University, 2021, 42(5): 12-17. doi: 10.12259/j.issn.2095-610X.S20210503
[7]	Liu Chong , Cao Hui , Yang Cai Cai , Wang Zhen . . Journal of Kunming Medical University, 2020, 41(06): 32-38.
[8]	Zhao Xian , Han Xue Song , Zhou Qing Zhu , Li Shan , Liu Liu , Wang Song Mei . The Bone Defect Repairment in Vivo with Co-cultured EPCs and BMSCs. Journal of Kunming Medical University, 2018, 39(03): 11-18.
[9]	Ji Qin Long , Kong Xiang Dong , Qi Shan Hong , Li Chao , Fan Jun , Sha Yong , Wang Shao Feng . The Experimental Study of Cyasterone on the Treatment of Osteoporosis through the Bidirectional Effect of Inhibiting Osteoclast Differetiation and Promoting Osteoblast Differentiation. Journal of Kunming Medical University, 2018, 39(05): 21-28.
[10]	Xu Lin , Hu Min , Li Zhong Hui , Fan Jian Nan . . Journal of Kunming Medical University, 2017, 38(02): 1-5.
[11]	Hu Zheng Xiong . Effect of TGF-β2 and geneX on the Proliferation and Osteogenic Differentiation of BrdU-labeled Bone Mesenchymal Stem Cells. Journal of Kunming Medical University,
[12]	Han Qun Chao . . Journal of Kunming Medical University,
[13]	Yao Han Xi . . Journal of Kunming Medical University,
[14]	Qi Zong Ze . . Journal of Kunming Medical University,
[15]	Yang De Biao . . Journal of Kunming Medical University,
[16]	Wu Bin . . Journal of Kunming Medical University,
[17]	. Inducing Differentiation of Pig's Bone Mesenchymal Stem Cell(BMSC) to Urothelial Epithelia in Vitro. Journal of Kunming Medical University,
[18]	Mao Xi Hong . Pathological Study of PNS in Regulating Cathepsin B Expression in Rabbits with Pulmonary Fibrosis of Pulmonary Heart Disease. Journal of Kunming Medical University,
[19]	. The Effect of Microarc Oxidation Treatment on the Surfaces of Polished and Sandblasted Titanium. Journal of Kunming Medical University,
[20]	Li Wei Guo . . Journal of Kunming Medical University,