Volume 44 Issue 3
Mar.  2023
Turn off MathJax
Article Contents
Jie LI, Yuan HE, Chunyan WU, Bin SHAN, Rui ZHENG, Zhifu GUAN, Baojun REN, Chunxia SHI, Youquan ZHOU, Hanyu LIU, Mi ZHANG, Yanming KANG, Guibo SONG. Distribution and Drug Resistance Analysis of Bacteria in Sterile Body Fluids in Yunnan from 2017 to 2021[J]. Journal of Kunming Medical University, 2023, 44(3): 61-67. doi: 10.12259/j.issn.2095-610X.S20230309
Citation: Jie LI, Yuan HE, Chunyan WU, Bin SHAN, Rui ZHENG, Zhifu GUAN, Baojun REN, Chunxia SHI, Youquan ZHOU, Hanyu LIU, Mi ZHANG, Yanming KANG, Guibo SONG. Distribution and Drug Resistance Analysis of Bacteria in Sterile Body Fluids in Yunnan from 2017 to 2021[J]. Journal of Kunming Medical University, 2023, 44(3): 61-67. doi: 10.12259/j.issn.2095-610X.S20230309

Distribution and Drug Resistance Analysis of Bacteria in Sterile Body Fluids in Yunnan from 2017 to 2021

doi: 10.12259/j.issn.2095-610X.S20230309
  • Received Date: 2022-11-14
    Available Online: 2023-03-02
  • Publish Date: 2023-03-25
  •   Objective  To explore the bacterial distribution and drug resistance of sterile body fluid specimens (excluding blood) in nine tertiary general hospitals in Yunnan Province from 2017-2021, and to provide a reference basis for the rational clinical use of antibiotics in the region.   Methods  Repeated strains from the same patients were excluded, and drug sensitivity tests were performed by instrumental method or paper method, and the results were interpreted according to the 2021 CLSI standard.   Results  Ascites was the specimen type with the highest strain isolation rate, accounting for 32.7%. A total of 5305 strains were isolated from all specimens, with Gram-positive bacteria accounting for 53.3%, predominantly Staphylococcus epidermidis, and Gram-negative bacteria accounting for 46.7%, predominantly Escherichia coli. Methicillin-resistant coagulase-negative staphylococci and methicillin-resistant Staphylococcus aureus were comparable in both cases and should not be underestimated. Enterococcus faecalis showed a decreasing trend of resistance to commonly used antimicrobials. The resistance rates of Klebsiella pneumoniae to meropenem and imipenem were 35.6% and 40.3%, respectively, both of which were higher than the 2021 CHINET level, and the resistance rates showed a trend of increasing year by year. Acinetobacter baumannii was highly resistant to most antibiotics, especially to carbapenems with a resistance rate of 82%.   Conclusion  The source of pathogenic bacteria in sterile body fluid is extensive, and the form of carbapenem resistance among different genera is severe. It is still necessary to focus on monitoring the third-generation cephalosporin-resistant Enterobacteriaceae and carbapenem-resistant Enterobacteriaceae and Acinetobacter baumannii.
  • loading
  • [1]
    Clinical Laboratory Standard Institute. Performance standards for antimicrobiol susceptibility[S]. 2021: M100-S31.
    [2]
    钟敏,龙姗姗,黄湘宁,等. 2015—2018年四川地区无菌体液细菌的分布及耐药性分析[J]. 中国抗生素杂志,2019,44(10):1181-1188. doi: 10.3969/j.issn.1001-8689.2019.10.011
    [3]
    黄燕春,陈知行,谢轶,等. 2012年3家教学医院无菌体液病原菌分布和耐药性[J]. 中国抗生素杂志,2014,39(6):456-461. doi: 10.3969/j.issn.1001-8689.2014.06.011
    [4]
    刘爽,肖晓光,林琳. 2013-2015年无菌体液病原菌分布及耐药性分析[J]. 检验医学与临床,2017,14(14):2038-2041. doi: 10.3969/j.issn.1672-9455.2017.14.013
    [5]
    臧婉,殷勤,何建维,等. 2013-2019年无菌体液病原菌分布及耐药性分析[J]. 国际检验医学杂志,2020,41(1):65-68,73. doi: 10.3969/j.issn.1673-4130.2020.01.017
    [6]
    秦立霞,李芳,赵会海. 感染性无菌体液细菌分布及耐药性分析[J]. 临床误诊误治,2020,33(3):77-82. doi: 10.3969/j.issn.1002-3429.2020.03.018
    [7]
    郭燕,吴湜,傅祝英杰,等. 血液和无菌体液分离金黄色葡萄球菌对抗菌药物的敏感性及毒力基因检测[J]. 中国感染与化疗杂志,2017,17(2):127-133. doi: 10.16718/j.1009-7708.2017.02.002
    [8]
    胡付品,郭燕,朱德妹,等. 2021年CHINET中国细菌耐药监测[J]. 中国感染与化疗杂志,2022,22(5):521-530.
    [9]
    喻华,徐雪松,李敏,等. 肠杆菌目细菌碳青霉烯酶的实验室检测和临床报告规范专家共识[J]. 中国感染与化疗杂志,2020,20(6):671-680. doi: 10.16718/j.1009-7708.2022.04.014
    [10]
    周文艳,张华,许永杰. 111例无菌体液标本中CRE的碳青霉烯酶检测及同源性分析[J]. 国际检验医学杂志,2022,43(12):1420-1424,1429. doi: 10.3969/j.issn.1673-4130.2022.12.003
    [11]
    Sheu C C,Chang Y T,Lin S Y,et al. Infections caused by carbapenem-resistant enterobacteriaceae: An update on therapeutic options[J]. Frontiers in Microbiology,2019,10:80. doi: 10.3389/fmicb.2019.00080
    [12]
    Karaiskos I,Galani I,Papoutsaki V,et al. Carbapenemase producing Klebsiella pneumoniae: implication on future therapeutic strategies[J]. Expert Review of Anti-Infective Therapy,2022,20(1):53-69. doi: 10.1080/14787210.2021.1935237
    [13]
    Ramirez M S,Bonomo R A,Tolmasky M E. Carbapenemases: Transforming acinetobacter baumannii into a yet more dangerous menace[J]. Biomolecules,2020,10(5):720. doi: 10.3390/biom10050720
    [14]
    Nasr P. Genetics,epidemiology,and clinical manifestations of multidrug-resistant Acinetobacter baumannii[J]. The Journal of Hospital Infection,2020,104(1):4-11. doi: 10.1016/j.jhin.2019.09.021
    [15]
    Ni W,Cui J,LiaNg B,et al. In vitro effects of tigecycline in combination with colistin (polymyxin E) and sulbactam against multidrug-resistant Acinetobacter baumannii[J]. The Journal of Antibiotics,2013,66(12):705-708. doi: 10.1038/ja.2013.84
    [16]
    Cai X,Yang z,Dai J,et al. Pharmacodynamics of tigecycline alone and in combination with colistin against clinical isolates of multidrug-resistant Acinetobacter baumannii in an in vitro pharmacodynamic model[J]. International Journal of Antimicrobial Agents,2017,49(5):609-616. doi: 10.1016/j.ijantimicag.2017.01.007
    [17]
    Liu C,Chen K,Wu Y,et al. Epidemiological and genetic characteristics of clinical carbapenem-resistant Acinetobacter baumannii strains collected countrywide from hospital intensive care units (ICUs) in China[J]. Emerging Microbes & Infections,2022,11(1):1730-1741.
    [18]
    Somily A,Balkhy H H,Enani M A S,et al. Antimicrobial resistance trends of non-fermenter Gram negative bacteria in Saudi Arabia: A six-year national study[J]. Journal of Infection and Public Health,2021,14(9):1144-1150. doi: 10.1016/j.jiph.2021.07.007
    [19]
    Seifert H,Blondeau J,Lucaßen K,et al. Global update on the in vitro activity of tigecycline and comparators against isolates of Acinetobacter baumannii and rates of resistant phenotypes (2016-2018)[J]. Journal of Global Antimicrobial Resistance,2022,31:82-89. doi: 10.1016/j.jgar.2022.08.002
    [20]
    Tacconelli E,Carrara E,Savoldi A,et al. Discovery,research,and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis[J]. The Lancet Infectious Diseases,2018,18(3):318-327. doi: 10.1016/S1473-3099(17)30753-3
  • Relative Articles

    [1] Feixue QIN, Juankun HE, Shi LIU, Bin WEN, Hong ZHU, Jing LI, Li GUI, Xiaoyan CAO. Study on the Distribution Characteristics of Pathogens in Hospitalized Diabetic Foot Patients and Its Correlation with Different Wagner Grades. Journal of Kunming Medical University, 2024, 46(): 1-7.
    [2] Yanzhu YIN, Zushun YANG, Yanqiushuo ZOU, Xiang REN, Yidan GUO, Lu FAN. Investigation of Molecular Typing and Drug Resistance of Shigella in Yunnan Province from 2016 to 2022. Journal of Kunming Medical University, 2024, 45(7): 79-86.  doi: 10.12259/j.issn.2095-610X.S20240712
    [3] Xuelin ZHANG, Lu LIU, Yanzhi CHEN, Caijun ZHA, Yanli LI. The Distribution and Drug Resistance of Pathogenic Bacteria in Respiratory Tract Infections in Children from 2019 to 2022. Journal of Kunming Medical University, 2024, 45(1): 149-155.  doi: 10.12259/j.issn.2095-610X.S20240125
    [4] Xiaoyan WANG, Ya LI, Chenglu HE, Lin WANG, Zhiqiang MA, Xinrui WAN. Study on Immune Function and Drug Resistance of Tuberculosis Patients Co-infected with HBV,HCV and HIV in Yunnan. Journal of Kunming Medical University, 2023, 44(12): 51-58.  doi: 10.12259/j.issn.2095-610X.S20231209
    [5] Xinrui WAN, Xiaoyan WANG, Lin WANG, Zhiqiang MA, Caixin LI, Shitao XU, Xingrui TANG, Hongyan Mao. Liver,Kidney and Immunologic Function and Drug Resistance in Patients with Nontuberculous Mycobacteria Infection. Journal of Kunming Medical University, 2023, 44(11): 113-119.  doi: 10.12259/j.issn.2095-610X.S20231117
    [6] Liju ZHANG, Xiaoming JIANG, Changxian CHEN, Xi WU, Zhenyong ZHANG, Weijun LIU. Long Non-coding RNA-p21 Regulates the MicroRNA-9 / Sirtuin-1 Signaling Pathway to Reverse Oxaliplatin Resistance in Colorectal Cancer Cells. Journal of Kunming Medical University, 2022, 43(5): 27-32.  doi: 10.12259/j.issn.2095-610X.S20220519
    [7] Hai-lin HUANG, Hong-chao JIANG, Xiao-juan LI. Drug Resistance of Escherichia coli and Klebsiella pneumoniae in Children with Bloodstream Infection from 2017 to 2020. Journal of Kunming Medical University, 2021, 42(8): 95-100.  doi: 10.12259/j.issn.2095-610X.S20210817
    [8] Zheng Zhi Rong , Shao Rui Fei , Zhao Shi Ming , Zhang Yi Xin , Chen Guo Bing . . Journal of Kunming Medical University, 2020, 41(05): 91-95.
    [9] Chen Jing Yi , Liao Fei , Feng Lei , Chu Cong Jia , Tian Ji Ye . Clinical Distribution and Drug Resistance of Pseudomonas aeruginosa Infection in Patients of Different Ages. Journal of Kunming Medical University, 2019, 40(03): 53-57.
    [10] Gong Rong , Sun Wei , Xu Cong Qiong , Liu Lin , Wang Juan , Jin Zhen . . Journal of Kunming Medical University, 2019, 40(05): 73-79.
    [11] Liu Shu Min . Distribution and Drug Resistance of Carbapenem-resistant Citrobacter Freundii from 2012 to 2014. Journal of Kunming Medical University,
    [12] Jing Xiao Jie . . Journal of Kunming Medical University,
    [13] Zhang He Yun . . Journal of Kunming Medical University,
    [14] Zhao Xiao Li . . Journal of Kunming Medical University,
    [15] Qi Yan Wei . 云南省HIV/AIDS患者下呼吸道感染病原菌分布及耐药分析. Journal of Kunming Medical University,
    [16] Sheng Xiao Cui . . Journal of Kunming Medical University,
    [17] Hu Yu Hong . . Journal of Kunming Medical University,
    [18] Mao Jian . . Journal of Kunming Medical University,
    [19] Zhou Shi Qiao . . Journal of Kunming Medical University,
    [20] . . Journal of Kunming Medical University,
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Tables(6)

    Article Metrics

    Article views (3078) PDF downloads(10) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return