Effect and Mechanism of Twist1 Regulating Bmi1 on Invasion and Migration of Gallbladder Carcinoma Cells

Dongxing XU; Bo TANG; Guo ZHU; Xuefen LEI; Qiuhong WANG; Dong WEI

doi:10.12259/j.issn.2095-610X.S20230320

Volume 44 Issue 3

Mar. 2023

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Dongxing XU, Bo TANG, Guo ZHU, Xuefen LEI, Qiuhong WANG, Dong WEI. Effect and Mechanism of Twist1 Regulating Bmi1 on Invasion and Migration of Gallbladder Carcinoma Cells[J]. Journal of Kunming Medical University, 2023, 44(3): 28-33. doi: 10.12259/j.issn.2095-610X.S20230320

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Effect and Mechanism of Twist1 Regulating Bmi1 on Invasion and Migration of Gallbladder Carcinoma Cells

doi: 10.12259/j.issn.2095-610X.S20230320

The Fourth Ward of Hepatobiliary and Pancreatic Surgery，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101，China

Received Date: 2022-11-10
Available Online: 2023-03-01
Publish Date: 2023-03-25

Abstract

Abstract

Objective To investigate the effect of Twist1 on invasion and metastasis of gallbladder carcinoma cells and the regulation of Bmi1, E-Cadherine andN-Cadherine. Methods Twist1 shRNA lentivirus vector was constructed to infect GBC-SD cells as the experimental group. The empty lentivirus-infected GBC-SD cells were the negative group, and the untreated GBC -SD cells were the blank group. Cell migration and invasion ability were detected by cell scratch and Transwell chamber invasion assay. Western blot and RT-PCR were used to detect the relative expression levels of Twist1, Bmi1, E-Cadherine and N-Cadherine. Results Compared with the negative and blank groups, the experimental group had a lower cell scratch healing degree and fewer invasion numbers （P < 0.0001）. There was no significant difference in cell migration rate and invasion number between the two control groups （P > 0.05）. The relative expression levels of Twist1, Bmi1 and N-Cadherine in the negative group and blank group were obviously higher than those in the experimental group （P < 0.001）. The relative expression levels of E-Cadherine protein and mRNA were obviously lower than those of the experimental group （P < 0.0001）. There was no significant difference in the relative expression levels of protein and mRNA between the negative group and the blank group （P > 0.05）. Conclusion Targeted silencing of Twist1 can inhibit the migration and invasion of gallbladder carcinoma cells, and the mechanism may be related to inhibiting the expression of Bmi1 and the process of epithelial-mesenchymal transformation.
- Gallbladder neoplasm,
- Twist1,
- Bmi1,
- Epithelial-mesenchymal transition,
- Invasion,
- Metastasis

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References(18)

References

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