LINC00341 Inhibits the Proliferation of Lung Adenocarcinoma Cells Through the MAPK Pathway

Shiqi MA; Yi DING; Min LI; Mengci WANG; Siyu ZHANG; Shumei FENG

doi:10.12259/j.issn.2095-610X.S20230403

Volume 44 Issue 4

Apr. 2023

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Shiqi MA, Yi DING, Min LI, Mengci WANG, Siyu ZHANG, Shumei FENG. LINC00341 Inhibits the Proliferation of Lung Adenocarcinoma Cells Through the MAPK Pathway[J]. Journal of Kunming Medical University, 2023, 44(4): 1-8. doi: 10.12259/j.issn.2095-610X.S20230403

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Shiqi MA, Yi DING, Min LI, Mengci WANG, Siyu ZHANG, Shumei FENG. LINC00341 Inhibits the Proliferation of Lung Adenocarcinoma Cells Through the MAPK Pathway[J]. Journal of Kunming Medical University, 2023, 44(4): 1-8. doi: 10.12259/j.issn.2095-610X.S20230403

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LINC00341 Inhibits the Proliferation of Lung Adenocarcinoma Cells Through the MAPK Pathway

doi: 10.12259/j.issn.2095-610X.S20230403

Shiqi MA¹,
Yi DING²,
Min LI³,
Mengci WANG¹,
Siyu ZHANG¹,
Shumei FENG^{1, 4
,
,}

1.
Dept. of Histology and Embryology，School of Basic Medical Sciences，Xinjiang Medical University，Xinjiang Key Laboratory of Molecular Biology of Endemic Diseases，Urumqi 830011
2.
School of Basic Medical Sciences，Nanjing Medical University，Nanjing 211166
3.
Dept. of Anatomy，School of Basic Medical Sciences，Xinjiang Second Medical College，Urumqi 830011，China
4.
Cancer Center，University of Michigan，Ann Arbor，USA

Received Date: 2022-12-23
Publish Date: 2023-04-25

Abstract

Abstract

Objective To explore the underlying mechnisms and effect of LINC00341 on the proliferation of lung adenocarcinoma cells. Methods The expression of LINC00341 mRNA in lung adenocarcinoma was analyzed in public data set SEO and TCGA, and its diagnostic value was analyzed by ROC curve. Survival analysis was performed on RMA and OKAYAMA data, and RNA-seq validation and ROC curve analysis were performed on lung cancer samples from Hospital of University of Michigan, USA. The biological function of LINC00341 gene was analyzed by gene otology （GO）. The expression of LINC00341 was silenced by siRNA and cells were divided into control group （NT） and silenced group （siLINC00341） according to different treatments, and the transfection efficiency was verified. Cell proliferation was detected by WST-1 and plate cloning assay. RNA subcellular localization was detected for LINC00341 localization, and Western blot was used to detect the effect of LINC00341 on the expression level of proteins related to MAPK signaling pathway. Results Low expression of LINC00341 was found in lung adenocarcinoma tissues （P < 0.0001 for 3 data sets）. ROC curve analysis indicated that LINC00341 could be used as a biomarker for lung adenocarcinoma , and low expression of LINC00341 was associated with better prognosis in patients （ P = 0.004）. GO enrichment analysis showed that LINC00341 was mainly related to transcriptional regulation of RNA polymerase II promoter and protein binding. Silencing LINC00341 could promote the proliferation of lung adenocarcinoma cells （P < 0.05） and clone formation （ P < 0.05） , and the MAPK signaling pathway proteins STAT3 and ERK were activated （ P < 0.05）. RNA localization showed that LINC00341 was mainly distributed in the nuclear. Conclusion LINC00341 may regulate MAPK pathway at transcriptional level, thus inhibiting the development of lung adenocarcinoma, and can be used as an important diagnostic and prognostic marker for lung adenocarcinoma.
- Lung adenocarcinoma,
- LINC00341,
- MAPK

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References(33)

References

[1]	Sung H,Ferlay J,Siegel R L,et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin,2021,71(3):209-249. doi: 10.3322/caac.21660
[2]	Turner M C,Andersen Z J,Baccarelli A,et al. Outdoor air pollution and cancer: An overview of the current evidence and public health recommendations[J]. CA Cancer J Clin,2020, published online ahead of print.
[3]	Malhotra J,Malvezzi M,Negri E,et al. Risk factors for lung cancer worldwide[J]. Eur Respir J,2016,48(3):889-902.
[4]	Succony L,Rassl D M,Barker A P,et al. Adenocarcinoma spectrum lesions of the lung: Detection,pathology and treatment strategies[J]. Cancer Treat Rev,2021,99:102237. doi: 10.1016/j.ctrv.2021.102237
[5]	Guo C J,Ma X K,Xing Y H,et al. Distinct processing of lncRNAs contributes to non-conserved functions in stem cells[J]. Cell,2020,181(3):621-636.e622. doi: 10.1016/j.cell.2020.03.006
[6]	Qian X,Zhao J,Yeung P Y,et al. Revealing lncRNA structures and interactions by sequencing-based approaches[J]. Trends Biochem Sci,2019,44(1):33-52.
[7]	Nojima T,Proudfoot N J. Mechanisms of lncRNA biogenesis as revealed by nascent transcriptomics[J]. Nat Rev Mol Cell Biol,2022,23(6):389-406. doi: 10.1038/s41580-021-00447-6
[8]	Slack F J,Chinnaiyan A M. The role of non-coding RNAs in oncology[J]. Cell,2019,179(5):1033-1055. doi: 10.1016/j.cell.2019.10.017
[9]	Wang H,Meng Q,Qian J,et al. Review: RNA-based diagnostic markers discovery and therapeutic targets development in cancer[J]. Pharmacol Ther,2022,234:108123. doi: 10.1016/j.pharmthera.2022.108123
[10]	Bhan A,Soleimani M,Mandal S S. Long noncoding RNA and cancer: A new paradigm[J]. Cancer Res,2017,77(15):3965-3981. doi: 10.1158/0008-5472.CAN-16-2634
[11]	Li S,Chen S,Wang B,et al. The long noncoding RNA LINC00341 suppresses colorectal carcinoma by preventing cell migration and apoptosis[J]. Cell Biochem Funct,2020,38(3):266-274. doi: 10.1002/cbf.3473
[12]	Yang Q,Li X,Zhou Y,et al. A LINC00341-mediated regulatory pathway supports chondrocyte survival and may prevent osteoarthritis progression[J]. J Cell Biochem,2019,120(6):10812-10820. doi: 10.1002/jcb.28372
[13]	Pan L,Tan Y,Wang B,et al. Caspase recruitment domain containing protein 9 suppresses non-small cell lung cancer proliferation and invasion via inhibiting MAPK/p38 pathway[J]. Cancer Res Treat,2020,52(3):867-885. doi: 10.4143/crt.2019.606
[14]	Zheng G,Shen Z,Chen H,et al. Metapristone suppresses non-small cell lung cancer proliferation and metastasis via modulating RAS/RAF/MEK/MAPK signaling pathway[J]. Biomed Pharmacother,2017,90:437-445. doi: 10.1016/j.biopha.2017.03.091
[15]	Okayama H,Kohno T,Ishii Y,et al. Identification of genes upregulated in ALK-positive and EGFR/KRAS/ALK-negative lung adenocarcinomas[J]. Cancer Res,2012,72(1):100-111. doi: 10.1158/0008-5472.CAN-11-1403
[16]	Irizarry R A,Hobbs B,Collin F,et al. Exploration,normalization,and summaries of high density oligonucleotide array probe level data[J]. Biostatistics,2003,4(2):249-264. doi: 10.1093/biostatistics/4.2.249
[17]	Seo J S,Ju Y S,Lee W C,et al. The transcriptional landscape and mutational profile of lung adenocarcinoma[J]. Genome Res,2012,22(11):2109-2119. doi: 10.1101/gr.145144.112
[18]	Cancer genome atlas research network. Comprehensive molecular profiling of lung adenocarcinoma [J]. Nature, 2014, 511（7511）: 543-550.
[19]	Dhanasekaran S M,Balbin O A,Chen G,et al. Transcriptome meta-analysis of lung cancer reveals recurrent aberrations in NRG1 and Hippo pathway genes[J]. Nat Commun,2014,5:5893. doi: 10.1038/ncomms6893
[20]	Liao Y,Wang J,Jaehnig E J,et al. WebGestalt 2019: Gene set analysis toolkit with revamped UIs and APIs[J]. Nucleic Acids Res,2019,47(W1):W199-W205. doi: 10.1093/nar/gkz401
[21]	Yan H,Bu P. Non-coding RNA in cancer[J]. Essays Biochem,2021,65(4):625-639.
[22]	Zhang Y X,Yuan J,Gao Z M,et al. LncRNA TUC338 promotes invasion of lung cancer by activating MAPK pathway[J]. Eur Rev Med Pharmacol Sci,2018,22(2):443-449.
[23]	Hua Q,Jin M,Mi B,et al. LINC01123,a c-Myc-activated long non-coding RNA,promotes proliferation and aerobic glycolysis of non-small cell lung cancer through miR-199a-5p/c-Myc axis[J]. J Hematol Oncol,2019,12(1):91. doi: 10.1186/s13045-019-0773-y
[24]	Del Real A,López-Delgado L,Sañudo C,et al. Long noncoding RNAs as bone marrow stem cell regulators in osteoporosis[J]. DNA Cell Biol,2020,39(9):1691-1699. doi: 10.1089/dna.2020.5672
[25]	Wang L,He Y,Liu W,et al. Non-coding RNA LINC00857 is predictive of poor patient survival and promotes tumor progression via cell cycle regulation in lung cancer[J]. Oncotarget,2016,7(10):11487-11499. doi: 10.18632/oncotarget.7203
[26]	Fang J Y,Richardson B C. The MAPK signalling pathways and colorectal cancer[J]. Lancet Oncol,2005,6(5):322-327. doi: 10.1016/S1470-2045(05)70168-6
[27]	Liu F,Yang X,Geng M,et al. Targeting ERK,an Achilles' Heel of the MAPK pathway,in cancer therapy[J]. Acta Pharm Sin B,2018,8(4):552-562. doi: 10.1016/j.apsb.2018.01.008
[28]	Ullah R,Yin Q,Snell A H,et al. RAF-MEK-ERK pathway in cancer evolution and treatment[J]. Semin Cancer Biol,2022,85:123-154.
[29]	Yang S H,Sharrocks A D,Whitmarsh A J. MAP kinase signalling cascades and transcriptional regulation[J]. Gene,2013,513(1):1-13. doi: 10.1016/j.gene.2012.10.033
[30]	Ye D,Li Y,Zhang H,et al. Silencing PRSS1 suppresses the growth and proliferation of gastric carcinoma cells via the ERK pathway[J]. Int J Biol Sci,2021,17(4):957-971. doi: 10.7150/ijbs.52591
[31]	Pranteda A,Piastra V,Stramucci L,et al. The p38 MAPK signaling activation in colorectal cancer upon therapeutic treatments[J]. Int J Mol Sci,2020,21(8):2773. doi: 10.3390/ijms21082773
[32]	Feng S,Zhang J,Su W,et al. Overexpression of LINC00152 correlates with poor patient survival and knockdown impairs cell proliferation in lung cancer[J]. Sci Rep,2017,7(1):2982. doi: 10.1038/s41598-017-03043-x
[33]	Jiang Q G,Xiong C F,Lv Y X. Kin17 facilitates thyroid cancer cell proliferation,migration,and invasion by activating p38 MAPK signaling pathway[J]. Mol Cell Biochem,2021,476(2):727-739. doi: 10.1007/s11010-020-03939-9

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