Effects of Scutellarin on MMP2，MMP9 and TIMP-1 Protein in Liver of Obese Rats with Chronic Liver Injury by High Glucose and Fat Diet

Liyuan WANG; Zhenting YANG; Qingling LI; Haiwen LI; Zhirong ZHAO; Yongrui YANG; Shude LI

doi:10.12259/j.issn.2095-610X.S20230408

Volume 44 Issue 4

Apr. 2023

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Article Navigation > Journal of Kunming Medical University > 2023 > 44(4): 9-15

Liyuan WANG, Zhenting YANG, Qingling LI, Haiwen LI, Zhirong ZHAO, Yongrui YANG, Shude LI. Effects of Scutellarin on MMP2，MMP9 and TIMP-1 Protein in Liver of Obese Rats with Chronic Liver Injury by High Glucose and Fat Diet[J]. Journal of Kunming Medical University, 2023, 44(4): 9-15. doi: 10.12259/j.issn.2095-610X.S20230408

Citation:

Liyuan WANG, Zhenting YANG, Qingling LI, Haiwen LI, Zhirong ZHAO, Yongrui YANG, Shude LI. Effects of Scutellarin on MMP2，MMP9 and TIMP-1 Protein in Liver of Obese Rats with Chronic Liver Injury by High Glucose and Fat Diet[J]. Journal of Kunming Medical University, 2023, 44(4): 9-15. doi: 10.12259/j.issn.2095-610X.S20230408

Citation:

Liyuan WANG, Zhenting YANG, Qingling LI, Haiwen LI, Zhirong ZHAO, Yongrui YANG, Shude LI. Effects of Scutellarin on MMP2，MMP9 and TIMP-1 Protein in Liver of Obese Rats with Chronic Liver Injury by High Glucose and Fat Diet[J]. Journal of Kunming Medical University, 2023, 44(4): 9-15. doi: 10.12259/j.issn.2095-610X.S20230408

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Effects of Scutellarin on MMP2，MMP9 and TIMP-1 Protein in Liver of Obese Rats with Chronic Liver Injury by High Glucose and Fat Diet

doi: 10.12259/j.issn.2095-610X.S20230408

1.
School of Basic Medicine
2.
The 1st Clinical Medical College，Kunming Medical University，Kunming Yunnan 650500
3.
Dept. of Hepatology，The 3rd Hospital of Kunming/Yunnan Provincial Center for Infectious Diseases Clinical Medicine，Kunming Yunnan 650041，China

Received Date: 2023-01-05
Available Online: 2023-04-12
Publish Date: 2023-04-25

Abstract

Abstract

Objective To investigate the effect of scutellarin （SCU） on liver fibrosis in obese rats with chronic liver injury induced by high-fat and high-sugar diet, and to explore the possibility of SCU to ameliorate the degree of liver fibrosis by promoting the expression of MMP2 and MMP9 and inhibiting the expression of TIMP-1. Methods The model of chronic liver injury and liver fibrosis was induced by high-fat and high-sugar diet. After 8 weeks of intragastric administration of SCU and curcumin （CUR）, HE staining was used to observe the pathology of liver tissue; Masson staining was used to detect the deposition of collagen fibers in liver tissue; and western blot and immunohistochemical staining were used to observe the expression of TIMP-1, MMP2 and MMP9 in liver tissue. Results In the model group, hepatocytes were swollen with irregular hepatocyte morphology, a large number of fat vacuoles, and blue collagen fibers were deposited in the confluent area. After different doses of SCU and CUR intervention, the fat vacuoles were reduced, the swelling of hepatocytes was decreased, and the degree of fibrosis was improved. In liver tissue cells, the protein expressions of MMP2 and MMP9 were down-regulated in the model group （P < 0.01）, and up-regulated after treatment with different doses of SCU and CUR （P < 0.01）. The expression of TIMP-1 protein was up-regulated in the model group （P < 0.01）, and down-regulated after treatment with different doses of SCU and CUR （P < 0.01）, and the dose dependence was not significant. Conclusion SCU may improve liver fibrosis caused by obesity by promoting MMP2 and MMP9, inhibiting the expression of TIMP-1 protein and reducing the accumulation of ECM.
- Liver fibrosis in chronic liver injury,
- Scutellarin,
- MMPs,
- TIMPs

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References(23)

References

[1]	George J,Tsuchishima M,Tsutsumi M. Metabolism of N-nitrosodimethylamine,methylation of macromolecules,and development of hepatic fibrosis in rodent models[J]. J Mol Med (Berl),2020,98(9):1203-1213. doi: 10.1007/s00109-020-01950-7
[2]	Oakley F. Interrogating mechanisms of liver fibrosis with omics[J]. Nat Rev Gastroenterol Hepatol,2022,19(2):89-90. doi: 10.1038/s41575-021-00567-6
[3]	Jun J H,Park S Y,Park S,et al. Formyl peptide receptor 2 alleviates hepatic fibrosis in liver cirrhosis by vascular remodeling[J]. Int J Mol Sci,2021,22(4):2-13.
[4]	Perez-Is L,Collazos J,de la Fuente B,et al. 24-month decline of non-invasive liver fibrosis markers in HCV-mono and HCV/HIV coinfection after direct-acting antiviral therapy[J]. Sci Rep,2022,12(1):3828. doi: 10.1038/s41598-022-07548-y
[5]	George J,Tsutsumi M,Tsuchishima M. MMP-13 deletion decreases profibrogenic molecules and attenuates N-nitrosodimethylamine-induced liver injury and fibrosis in mice[J]. J Cell Mol Med,2017,21(12):3821-3835. doi: 10.1111/jcmm.13304
[6]	Xie H,Su D,Zhang J,et al. Raw and vinegar processed Curcuma wenyujin regulates hepatic fibrosis via bloking TGF-beta/Smad signaling pathways and up-regulation of MMP-2/TIMP-1 ratio[J]. J Ethnopharmacol,2020,246(1):111768.
[7]	Li X M,Peng J H,Sun Z L,et al. Chinese medicine CGA formula ameliorates DMN-induced liver fibrosis in rats via inhibiting MMP2/9,TIMP1/2 and the TGF-beta/Smad signaling pathways[J]. Acta Pharmacol Sin,2016,37(6):783-793. doi: 10.1038/aps.2016.35
[8]	Attia H,Al-Rasheed N,Mohamad R,et al. The antifibrotic and fibrolytic properties of date fruit extract via modulation of genotoxicity,tissue-inhibitor of metalloproteinases and nuclear factor- kappa B pathway in a rat model of hepatotoxicity[J]. BMC Complement Altern Med,2016,16(1):414. doi: 10.1186/s12906-016-1388-2
[9]	Peng L,Wen L,Shi Q F,et al. Scutellarin ameliorates pulmonary fibrosis through inhibiting NF-kappaB/NLRP3-mediated epithelial-mesenchymal transition and inflammation[J]. Cell Death Dis,2020,11(11):978. doi: 10.1038/s41419-020-03178-2
[10]	王银辉,耿玲,李辉. 灯盏乙素抗大鼠肝纤维化作用的研究[J]. 中国中药杂志,2015,40(10):1999-2003.
[11]	杨永锐,王丽媛,李海雯,等. 灯盏乙素抑制NOX的表达改善非酒精性脂肪性肝病肝脏纤维化的研究[J]. 昆明医科大学学报,2022,43(7):38-45.
[12]	Piche M E,Tchernof A,Despres J P. Obesity phenotypes,diabetes,and cardiovascular diseases[J]. Circ Res,2020,126(11):1477-1500. doi: 10.1161/CIRCRESAHA.120.316101
[13]	Polyzos S A,Kountouras J,Mantzoros C S. Obesity and nonalcoholic fatty liver disease:From pathophysiology to therapeutics[J]. Metabolism,2019,92(4):82-97.
[14]	Devaraj E,Perumal E,Subramaniyan R,et al. Liver fibrosis:Extracellular vesicles mediated intercellular communication in perisinusoidal space[J]. Hepatology,2022,76(1):275-285. doi: 10.1002/hep.32239
[15]	Roehlen N,Crouchet E,Baumert T F. Liver fibrosis:Mechanistic concepts and therapeutic perspectives[J]. Cells,2020,9(4):2-43.
[16]	Aydin M M,Akcali K C. Liver fibrosis[J]. Turk J Gastroenterol,2018,29(1):14-21. doi: 10.5152/tjg.2018.17330
[17]	李晋,徐尚福,李远洋,等. 小柴胡汤对肝纤维化大鼠肝脏MMP-2、TIMP-2表达的影响[J]. 辽宁中医杂志,2018,45(5):1066-1068. doi: 10.13192/j.issn.1000-1719.2018.05.053
[18]	Ye J,Zeng B,Zhong M,et al. Scutellarin inhibits caspase-11 activation and pyroptosis in macrophages via regulating PKA signaling[J]. Acta Pharm Sin B,2021,11(1):112-126. doi: 10.1016/j.apsb.2020.07.014
[19]	Miao Z,Lai Y,Zhao Y,et al. Scutellarein aggravated carbon tetrachloride-induced chronic liver injury in gut microbiota-dysbiosis mice[J]. Evid Based Complement Alternat Med,2020,2020(11):8811021.
[20]	Mansour-Ghanaei F,Pourmasoumi M,Hadi A,et al. Efficacy of curcumin/turmeric on liver enzymes in patients with non-alcoholic fatty liver disease: A systematic review of randomized controlled trials[J]. Integr Med Res,2019,8(1):57-61. doi: 10.1016/j.imr.2018.07.004
[21]	Dkab C,Zzb C,Lc A,et al. Curcumin blunts epithelial-mesenchymal transition of hepatocytes to alleviate hepatic fibrosis through regulating oxidative stress and autophagy[J]. Redox Biology,2020,36(5):101600.
[22]	Zhang L,Pan X,Xu L,et al. Mitochondria-targeted curcumin loaded CTPP-PEG-PCL self-assembled micelles for improving liver fibrosis therapy[J]. RSC Adv,2021,11(10):5348-5360. doi: 10.1039/D0RA09589C
[23]	Medeiros T,Saraiva G N,Moraes L A,et al. Liver fibrosis improvement in chronic hepatitis C after direct acting-antivirals is accompanied by reduced profibrogenic biomarkers-a role for MMP-9/TIMP-1[J]. Dig Liver Dis,2020,52(10):1170-1177. doi: 10.1016/j.dld.2020.05.004

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