Voluntary Exercise Training Attenuated the Proliferation and Growth of Human Breast Cancer with BRCA1 Mutation by Regulating Caspase-3 Activity

Tao HU; Yi WU; Wenda GENG; Yijian ZHANG; Xuan HE; Shanshan LI; Yanbin XIYANG; Liling DENG

doi:10.12259/j.issn.2095-610X.S20230419

Volume 44 Issue 4

Apr. 2023

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Article Navigation > Journal of Kunming Medical University > 2023 > 44(4): 31-39

Tao HU, Yi WU, Wenda GENG, Yijian ZHANG, Xuan HE, Shanshan LI, Yanbin XIYANG, Liling DENG. Voluntary Exercise Training Attenuated the Proliferation and Growth of Human Breast Cancer with BRCA1 Mutation by Regulating Caspase-3 Activity[J]. Journal of Kunming Medical University, 2023, 44(4): 31-39. doi: 10.12259/j.issn.2095-610X.S20230419

Citation:

Tao HU, Yi WU, Wenda GENG, Yijian ZHANG, Xuan HE, Shanshan LI, Yanbin XIYANG, Liling DENG. Voluntary Exercise Training Attenuated the Proliferation and Growth of Human Breast Cancer with BRCA1 Mutation by Regulating Caspase-3 Activity[J]. Journal of Kunming Medical University, 2023, 44(4): 31-39. doi: 10.12259/j.issn.2095-610X.S20230419

Citation:

Tao HU, Yi WU, Wenda GENG, Yijian ZHANG, Xuan HE, Shanshan LI, Yanbin XIYANG, Liling DENG. Voluntary Exercise Training Attenuated the Proliferation and Growth of Human Breast Cancer with BRCA1 Mutation by Regulating Caspase-3 Activity[J]. Journal of Kunming Medical University, 2023, 44(4): 31-39. doi: 10.12259/j.issn.2095-610X.S20230419

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Voluntary Exercise Training Attenuated the Proliferation and Growth of Human Breast Cancer with BRCA1 Mutation by Regulating Caspase-3 Activity

doi: 10.12259/j.issn.2095-610X.S20230419

1.
Dept. of Laboratory Medicine，The 3rd People’s Hospital of Yunnan Province，Kunming Yunnan 650200
2.
Science and Technology Achievement Incubation Center
3.
School of Basic Medicine Kunming Medical University，Kunming Medical University，Kunming Yunnan 650500
4.
Dept. of Gynecology，Hunan Provincial TCM Hospital， Zhuzhou Hunan 412008, China

Received Date: 2023-01-17
Available Online: 2023-04-24
Publish Date: 2023-04-25

Abstract

Abstract

Objective This study aims to explore the role and mechanism of voluntary exercise training in the development and progression of breast cancer. Methods Primary human Breast Cancer gene 1-mutation （BRCA-1 mutation） cancer cells were cultured in vitro, before treated with the serum of wheel running rats and sedentary rats for 14 consecutive days. The cells cultured in above conditions were injected subcutaneously into BALB/c nude mice to observe the in vitro and in vivo breast cancer cells with BRCA-1 mutation and tumor transplantation after different interventions. Results In vitro, compared with sedentary rat serum group, the activity and growth of BRCA-1 mutant breast cancer cells were inhibited and apoptosis was promoted after in the wheel running group. In vivo, the grafts of BRCA-1 mutated breast cancer cells cultured from in the wheel running group decreased in volume and grew slower compared with the sedentary group, and the wheel running group showed enhanced Caspase-3 activity both in vivo and in vitro. Conclusion Voluntary wheel running may attenuate the growth of BRCA1 mutant breast cancer cells and their proliferation and apoptosis in BALB/c nude mice by enhancing the activity of Caspase3, thus inhibiting the growth of breast cancer cells in vitro and in vivo.
- Breast cancer,
- Breast Cancer （BRCA） gene 1,
- Voluntary running wheel,
- Proliferation,
- Apoptosis

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References(30)

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