Effects of Store-operated Calcium Channel Molecule Orai3  on Rat Coronary Artery Smooth Muscle Cells Proliferation

Hui WANG; Wenjun ZENG; Haiping ZHANG; Ruiwei GUO

doi:10.12259/j.issn.2095-610X.S20230423

Volume 44 Issue 4

Apr. 2023

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Hui WANG, Wenjun ZENG, Haiping ZHANG, Ruiwei GUO. Effects of Store-operated Calcium Channel Molecule Orai3 on Rat Coronary Artery Smooth Muscle Cells Proliferation[J]. Journal of Kunming Medical University, 2023, 44(4): 16-22. doi: 10.12259/j.issn.2095-610X.S20230423

Citation:

Hui WANG, Wenjun ZENG, Haiping ZHANG, Ruiwei GUO. Effects of Store-operated Calcium Channel Molecule Orai3 on Rat Coronary Artery Smooth Muscle Cells Proliferation[J]. Journal of Kunming Medical University, 2023, 44(4): 16-22. doi: 10.12259/j.issn.2095-610X.S20230423

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Effects of Store-operated Calcium Channel Molecule Orai3 on Rat Coronary Artery Smooth Muscle Cells Proliferation

doi: 10.12259/j.issn.2095-610X.S20230423

Dept. of Cardiology，920th Hospital of Joint Logistics Support Force，PLA， Kunming Yunnan 650032，China

Received Date: 2023-01-30
Available Online: 2023-04-18
Publish Date: 2023-04-25

Abstract

Abstract

Objective To investigate the effect of calcium store-operated calcium channel Orai3 on the proliferation of coronary artery smooth muscle cells （CASMC）. Methods Coronary artery smooth muscle cells （CASMC） were cultured, and Orai3 molecule in CASMC was interfered and over-expressed. QPCR and Western Blot were used to detect the expression of Orai3, and CCK8 and cell counts were used to detect the CASMC proliferation. Results CCK8 assay and cell counts showed that the proliferation rate of CASMC in serum-free group was lower than that in 10% serum group （P < 0.05）, and also the expression of Orai3 in CASMC was decreased （P < 0.05）. Compared with the control group, the proliferation rate of CASMC decreased after interference with Orai3 （P < 0.05）, and the proliferation rate of CASMC increased compared with the control group after over-expression of Orai3 （P < 0.05）. Conclusion The store-operated calcium channel constituent molecule Orai3 plays an important role in CASMC proliferation.
- Coronary artery smooth muscle cell,
- Cell proliferation,
- Orai3,
- Store-operated calcium channel

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References(14)

References

[1]	Bennett M R,Sinha S,Owens G K. Vascular smooth muscle cells in atherosclerosis[J]. Circ Res,2016,118(4):692-702. doi: 10.1161/CIRCRESAHA.115.306361
[2]	Guo R W,Wang H,Gao P,et al. An essential role for stromal interaction molecule 1 in neointima formation following arterial injury[J]. Cardiovasc Res,2009,81(4):660-668. doi: 10.1093/cvr/cvn338
[3]	Mammadova-Bach E,Nagy M,Heemskerk J W M,et al. Store-operated calcium entry in thrombosis and thrombo-inflammation[J]. Cell Calcium,2019,77:39-48. doi: 10.1016/j.ceca.2018.11.005
[4]	Fahrner M, Schindl R, Romanin C. Studies of structure-function and subunit composition of Orai/STIM channel[M]. Calcium Entry Channels in Non-Excitable Cells. Boca Raton （FL）: CRC Press/Taylor & Francis, 2018: 25-50.
[5]	Tanwar J,Motiani R K. Role of SOCE architects STIM and Orai proteins in cell death[J]. Cell Calcium,2018,69:19-27. doi: 10.1016/j.ceca.2017.06.002
[6]	Sanchez-Collado J,Jardin I,López J J,et al. Role of Orai3 in the pathophysiology of cancer[J]. Int J Mol Sci,2021,22(21):11426. doi: 10.3390/ijms222111426
[7]	Zimmermann O,Zwaka T P,Marx N,et al. Serum starvation and growth factor receptor expression in vascular smooth muscle cells[J]. J Vasc Res,2006,43(2):157-165. doi: 10.1159/000090945
[8]	Codeluppi S,Gregory E N,Kjell J,et al. Influence of rat substrain and growth conditions on the characteristics of primary cultures of adult rat spinal cord astrocytes[J]. J Neurosci Methods,2011,197(1):118-127. doi: 10.1016/j.jneumeth.2011.02.011
[9]	Pinto M C,Kihara A H,Goulart V A,et al. Calcium signaling and cell proliferation[J]. Cellular signalling,2015,27(11):2139-2149. doi: 10.1016/j.cellsig.2015.08.006
[10]	Shawer H,Norman K,Cheng C W,et al. ORAI1 Ca²⁺ channel as a therapeutic target in pathological vascular remodelling[J]. Front Cell Dev Biol,2021,9:653812. doi: 10.3389/fcell.2021.653812
[11]	Sanchez-Collado J,Lopez J J,Cantonero C,et al. Orai2 modulates Store-Operated Ca²⁺ entry and cell cycle progression in breast cancer cells[J]. Cancers,2021,14(1):114. doi: 10.3390/cancers14010114
[12]	Borowiec A S,Bidaux G,Tacine R,et al. Are Orail and Orai3 channels more important than calcium influx for cell proliferation?[J]. Biochim Biophys Acta,2014,1843(2):464-472. doi: 10.1016/j.bbamcr.2013.11.023
[13]	Dubois C,Vanden Abeele F,Lehen'kyi V,et al. Remodeling of channel-forming ORAI proteins determines an oncogenic switch in prostate cance[J]. Cancer Cell,2014,26(1):19-32. doi: 10.1016/j.ccr.2014.04.025
[14]	Dubois C,Kondratska K,Kondratskyi A,et al. ORAI3 silencing alters cell proliferation and promotes mitotic catastrophe and apoptosis in pancreatic adenocarcinoma[J]. Biochim Biophys Acta Mol Cell Res,2021,1868(7):119023. doi: 10.1016/j.bbamcr.2021.119023

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