The Regulation of VEGF Signal Axis by DATS and the Improvement of Formation of Placental Angiogenesis in URSA Mice

Jiamei SONG; Yang LIU; Tao SHI; Fangjie JIANG; Jingsi CHEN; Wen FAN; Yu LIANG; Yushi MENG

doi:10.12259/j.issn.2095-610X.S20230506

Volume 44 Issue 5

May 2023

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Jiamei SONG, Yang LIU, Tao SHI, Fangjie JIANG, Jingsi CHEN, Wen FAN, Yu LIANG, Yushi MENG. The Regulation of VEGF Signal Axis by DATS and the Improvement of Formation of Placental Angiogenesis in URSA Mice[J]. Journal of Kunming Medical University, 2023, 44(5): 6-11. doi: 10.12259/j.issn.2095-610X.S20230506

Citation:

Jiamei SONG, Yang LIU, Tao SHI, Fangjie JIANG, Jingsi CHEN, Wen FAN, Yu LIANG, Yushi MENG. The Regulation of VEGF Signal Axis by DATS and the Improvement of Formation of Placental Angiogenesis in URSA Mice[J]. Journal of Kunming Medical University, 2023, 44(5): 6-11. doi: 10.12259/j.issn.2095-610X.S20230506

Citation:

Jiamei SONG, Yang LIU, Tao SHI, Fangjie JIANG, Jingsi CHEN, Wen FAN, Yu LIANG, Yushi MENG. The Regulation of VEGF Signal Axis by DATS and the Improvement of Formation of Placental Angiogenesis in URSA Mice[J]. Journal of Kunming Medical University, 2023, 44(5): 6-11. doi: 10.12259/j.issn.2095-610X.S20230506

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The Regulation of VEGF Signal Axis by DATS and the Improvement of Formation of Placental Angiogenesis in URSA Mice

doi: 10.12259/j.issn.2095-610X.S20230506

1.
Dept. of Reproductive Medicine
2.
Dept. of Pain，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101，China

Received Date: 2023-01-19
Available Online: 2023-05-11
Publish Date: 2023-05-25

Abstract

Abstract

Objective The aim of this study is to investigate the effect of Diallyl trisulfide （DATS） on placental angiogenesis in mice with unexplained recurrent spontaneous abortion （URSA） and to explore its mechanism based on the vascular endothelial growth factor （VEGF）/vascular endothelial growth factor receptor-2 （VEGFR-2） signaling pathway. Methods Sixteen URSA mice selected were randomly divided into the control group and the experimental group, with 8 mice in each group. The experimental group was given 98% pure DATS （200 μL suspension prepared according to 50 mg/（kg·d) by gavage. The rats in the control group were fed conventionally and given 200 μL PBS solution by gavage. After 18 days of continuous administration, Hydrogen sulfide （H₂S） content in the placental tissue was detected by spectrophotometer, and the relative expression of CD31, VEGFA and VEGFR2 mRNA in the placental tissue was detected by reverse transcriptase-polymerase chain reaction （RT-qPCR）. The protein expression levels of VEGFA and VEGFR2 in placenta were detected by Western blot. Results The spectrophotometer detection showed that the H₂S level in the experimental group （777.4922±72.9759） was significantly higher than that in the control group （529.1824±99.7489）（P < 0.05）; The RT-qPCR results showed that the relative expression of CD31 in the experimental group （0.0042±0.0006） was significantly higher than that in the control group （0.0020±0.0004）（P < 0.05）, the relative expression of VEGFA in the experimental group （0.7073±0.0677） was significantly higher than that in the control group （0.5200±0.0946）（P < 0.05）, and the relative expression of VEGFR2 in the experimental group （0.7304±0.1262） was significantly higher than that in the control group （0.3984±0.047）（P < 0.05）; Western blot results showed that DATS significantly increased the protein expression levels of VEGFA and VEGFR2 in placental tissue （P < 0.05）. Conclusion DATS can significantly increase the level of H₂S in URSA placental tissue and the relative expression levels of angiogenic factors CD31, VEGFA, and VEGFR2 in placental tissue. Through the VEGF signal axis, it can improve the formation of placental blood vessels in URSA mice. The relevant research results will provide scientific basis and reference value for the clinical application of DATS.
- DATS,
- VEGF signaling axis,
- URSA mice,
- Placental vessels

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References(31)

References

[1]	Practice Committee of the American Society for Reproductive Medicine. Evaluation and treatment of recurrent pregnancy loss: A committee opinion[J]. Fertil Steril,2012,98(5):1103-1111. doi: 10.1016/j.fertnstert.2012.06.048
[2]	Bender Atik R,Christiansen O B,Elson J,et al. ESHRE guideline: Recurrent pregnancy loss: An update in 2022[J]. Hum Reprod Open,2023,2023(1):hoad002.
[3]	Hong Li Y,Marren A. Recurrent pregnancy loss: A summary of international evidence-based guidelines and practice[J]. Aust J Gen Pract,2018,47(7):432-436. doi: 10.31128/AJGP-01-18-4459
[4]	中华医学会妇产科学分会产科学组,复发性流产诊治专家共识编写组,张建平,等. 复发性流产诊治专家共识(2022)[J]. 中华妇产科杂志,2022,57(9):653-667. doi: 10.3760/cma.j.cn112141-20220421-00259
[5]	Muyayalo K P,Tao D,Lin X X,et al. Age-related changes in CD4+ T and NK cell compartments may contribute to the occurrence of pregnancy loss in advanced maternal age[J]. J Reprod Immunol,2023,155(2):103790.
[6]	Tise C G,Byers H M. Genetics of recurrent pregnancy loss: A review[J]. Curr Opin Obstet Gynecol,2021,33(2):106-111. doi: 10.1097/GCO.0000000000000695
[7]	Park S J,Min J Y,Kang J S,et al. Chromosomal abnormalities of 19,000 couples with recurrent spontaneous abortions: A multicenter study[J]. Fertil Steril,2022,117(5):1015-1025. doi: 10.1016/j.fertnstert.2022.01.011
[8]	Kim M A,Kim H S,Kim Y H. Reproductive,obstetric and neonatal outcomes in women with congenital uterine anomalies: A systematic review and meta-analysis[J]. J Clin Med,2021,10(21):4797. doi: 10.3390/jcm10214797
[9]	Practice committees of the American society for reproductive medicine and the society for reproductive endocrinology and infertility. Diagnosis and treatment of luteal phase deficiency: A committee opinion[J]. Fertil Steril,2021,115(6):1416-1423. doi: 10.1016/j.fertnstert.2021.02.010
[10]	复发性流产合并风湿免疫病免疫抑制剂应用中国专家共识编写组,吕良敬,赵爱民,等. 复发性流产合并风湿免疫病免疫抑制剂应用中国专家共识[J]. 中华生殖与避孕杂志,2020,40(7):527-534.
[11]	国家妇幼健康研究会生殖免疫学专业委员会专家共识编写组,赵爱民,韦相才. 复发性流产合并血栓前状态诊治中国专家共识[J]. 中华生殖与避孕杂志,2021,41(10):861-875.
[12]	邓玉梅,宋家美,刘仲伟,等. 淋巴细胞免疫治疗次数对复发性流产的疗效观察[J]. 昆明医科大学学报,2019,40(10):150-153. doi: 10.3969/j.issn.1003-4706.2019.10.033
[13]	Dakhly D M,Bayoumi Y A,Sharkawy M,et al. Intralipid supplementation in women with recurrent spontaneous abortion and elevated levels of natural killer cells[J]. Int J Gynaecol Obstet,2016,135(3):324-327. doi: 10.1016/j.ijgo.2016.06.026
[14]	Deshmukh H,Way S S. Immunological basis for recurrent fetal loss and pregnancy complications[J]. Annu Rev Pathol,2019,14(1):185-210. doi: 10.1146/annurev-pathmechdis-012418-012743
[15]	王班琴. H2S通过干扰滋养细胞入侵及调控母胎界面TH1/TH2平衡影响早孕的相关作用机制研究[D]. 济南: 山东大学博士学位论文, 2021.
[16]	Guerra D D,Hurt K J. Gasotransmitters in pregnancy: From conception to uterine involution[J]. Biol Reprod,2019,101(1):4-25. doi: 10.1093/biolre/ioz038
[17]	熊佳. 二烯丙基三硫醚对妊娠肥胖母鼠繁殖性能和胎盘血管发育的影响[D]. 武汉: 华中农业大学硕士学位论文, 2020.
[18]	Wang M,Wang Z,Miao Y,et al. Diallyl trisulfide promotes placental angiogenesis by regulating lipid metabolism and alleviating inflammatory responses in obese pregnant mice[J]. Nutrients,2022,14(11):2230. doi: 10.3390/nu14112230
[19]	杜珂珂,严育宏,廖鸿力,等. 不明原因复发性流产伴慢性子宫内膜炎诊断方法和高危因素分析[J]. 中国妇幼保健,2022,37(22):4230-4233. doi: 10.19829/j.zgfybj.issn.1001-4411.2022.22.032
[20]	Zhu L,Liu M,Zhang S,et al. Foxp3 TSDR Hypermethylation is correlated with decreased tregs in patients with unexplained recurrent spontaneous abortion[J]. Reprod Sci,2021,28(2):470-478. doi: 10.1007/s43032-020-00299-z
[21]	刘乐乐,路玥,王涛,等. MiRNA在不明原因复发性流产中的作用[J]. 生命的化学,2021,41(3):471-477. doi: 10.13488/j.smhx.20200500
[22]	Ravikumar G,Mukhopadhyay A,Mani C,et al. Placental expression of angiogenesis-related genes and their receptors in IUGR pregnancies: Correlation with fetoplacental and maternal parameters[J]. J Matern Fetal Neonatal Med,2020,33(23):3954-3961. doi: 10.1080/14767058.2019.1593362
[23]	Lu L,Kingdom J,Burton G J,et al. Placental stem villus arterial remodeling associated with reduced hydrogen sulfide synthesis contributes to human fetal growth restriction[J]. Am J Pathol,2017,187(4):908-920. doi: 10.1016/j.ajpath.2016.12.002
[24]	Li W,Chen P,Pan Y,et al. Construction of a band-aid like cardiac patch for myocardial infarction with controllable H₂S release[J]. Adv Sci (Weinh),2022,9(35):e2204509. doi: 10.1002/advs.202204509
[25]	Li M,Liu Y,Deng Y,et al. Therapeutic potential of endogenous hydrogen sulfide inhibition in breast cancer (Review)[J]. Oncol Rep,2021,45(5):68. doi: 10.3892/or.2021.8019
[26]	Matin M,Mörgelin M,Stetefeld J,et al. Affinity-enhanced multimeric VEGF (vascular endothelial growth factor) and PlGF (placental growth factor) variants for specific adsorption of sFlt-1 to restore angiogenic balance in preeclampsia[J]. Hypertension,2020,76(4):1176-1184. doi: 10.1161/HYPERTENSIONAHA.120.14974
[27]	刘鹏刚,刘彩虹,包文斌,等. 二花脸猪隐睾和正常睾丸组织学观察及PCNA/CD31蛋白差异表达分析[J]. 中国兽医科学,2023,53(3):388-395. doi: 10.16656/j.issn.1673-4696.2023.0036
[28]	夏士涛,王培宇,魏静,等. 海风藤醇提物抑制CD31因子对CSDH模型大鼠血肿外膜血管炎症影响的研究[J]. 中医药学报,2022,50(11):37-41. doi: 10.19664/j.cnki.1002-2392.220246
[29]	Smink A M,Najdahmadi A,Alexander M,et al. The effect of a fast-releasing hydrogen sulfide donor on vascularization of subcutaneous scaffolds in immunocompetent and immunocompromised mice[J]. Biomolecules,2020,10(5):722. doi: 10.3390/biom10050722
[30]	Guo X,Yi H,Li T C,et al. Role of vascular endothelial growth factor (VEGF) in human embryo implantation: Clinical implications[J]. Biomolecules,2021,11(2):253. doi: 10.3390/biom11020253
[31]	谢斌. CLEC14A通过VEGFA/VEGFR-2信号通路调控婴幼儿血管瘤内皮细胞增殖和血管生成的机制研究[D]. 南昌: 南昌大学博士学位论文, 2022.

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