Clinical Value of Fecal SDC2 Gene Methylation Detection in Early Screening of Colorectal Cancer

Yuan WANG; Rong LI; Jiaojiao CHEN; Yanli SHEN; Fengdi HU

doi:10.12259/j.issn.2095-610X.S20230524

Volume 44 Issue 5

May 2023

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Article Navigation > Journal of Kunming Medical University > 2023 > 44(5): 138-143

Yuan WANG, Rong LI, Jiaojiao CHEN, Yanli SHEN, Fengdi HU. Clinical Value of Fecal SDC2 Gene Methylation Detection in Early Screening of Colorectal Cancer[J]. Journal of Kunming Medical University, 2023, 44(5): 138-143. doi: 10.12259/j.issn.2095-610X.S20230524

Citation:

Yuan WANG, Rong LI, Jiaojiao CHEN, Yanli SHEN, Fengdi HU. Clinical Value of Fecal SDC2 Gene Methylation Detection in Early Screening of Colorectal Cancer[J]. Journal of Kunming Medical University, 2023, 44(5): 138-143. doi: 10.12259/j.issn.2095-610X.S20230524

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Clinical Value of Fecal SDC2 Gene Methylation Detection in Early Screening of Colorectal Cancer

doi: 10.12259/j.issn.2095-610X.S20230524

1.
Kunming Medical University，Kunming Yunnan 650500
2.
Dept. of Gastroenteric Oncology，The 3rd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650118，China

Received Date: 2023-01-19
Publish Date: 2023-05-25

Abstract

Abstract

Objective This study aims to investigate the clinical application of fecal recombinant human adhesin syndecan 2 （SDC2） gene methylation test in the early screening of colorectal tumors. Methods Eighty-seven subjects admitted to the hospital from March 2021 to October 2022 were selected as the study subjects, including 41 cases with normal colorectum, 21 with colorectal adenoma and 25 with colorectal cancer. In this study, the fecal DNA of the subjects was extracted for the fecal SDC2 gene methylation test, and the negative and positive results of the tested specimens were determined according to the Cutoff value set by the kit. The effects of the SDC2 gene methylation test were compared with the results of colonoscopy diagnosis. The analysis of variance on SDC2 gene methylation cycle thresholds （Ct） was performed to assess the expression of SDC2 methylation in colorectal tumors. The subject operating characteristic curve （ROC） was used to calculate the area under the curve （AUC） to evaluate the diagnostic value of SDC2 gene methylation in colorectal tumors and to analyze the sensitivity and specificity of SDC2 gene methylation in colorectal tumor diagnosis. Results Among the 87 enrolled cases, 35 were positive for SDC2 gene methylationand 52 were negative. The positive rate in the stool of colorectal adenoma patients and colorectal cancer patients was 57.14% （12/21） and 84.00% （21/25）, respectively and there were statistically significant differences （P = 0.044）. The results of the analysis under the ROC curve showed that the area of SDC2 gene methylation under the curve （AUC） of the test for the diagnosis of colorectal adenoma and colorectal cancer was 0.715 and 0.918, respectively, with a sensitivity of 57.14% and 88.00% and a specificity of 95.12% and 92.68%, respectively and there were statistically significant differences （P = 0.0107, P < 0.0001）. Conclusion Fecal SDC2 gene methylation has a high specificity for detecting colorectal cancer and its precancerous lesions, and the sensitivity of detection increases as the cancer progresses, so it is of great clinical value in early screening of colorectal cancer.
- SDC2 gene,
- Colorectal cancer,
- Clinical value

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References(23)

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