Effects of Osteoking Combined with Antibiotic Cocktail on Insulin Resistance and Gut Flora in db/db Mice

Shaoyou DENG; Yulan ZHAO; Peijin WANG; Rong LI; Jintao LI; Hong ZHENG

doi:10.12259/j.issn.2095-610X.S20230530

Volume 44 Issue 5

May 2023

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Shaoyou DENG, Yulan ZHAO, Peijin WANG, Rong LI, Jintao LI, Hong ZHENG. Effects of Osteoking Combined with Antibiotic Cocktail on Insulin Resistance and Gut Flora in db/db Mice[J]. Journal of Kunming Medical University, 2023, 44(5): 12-18. doi: 10.12259/j.issn.2095-610X.S20230530

Citation:

Shaoyou DENG, Yulan ZHAO, Peijin WANG, Rong LI, Jintao LI, Hong ZHENG. Effects of Osteoking Combined with Antibiotic Cocktail on Insulin Resistance and Gut Flora in db/db Mice[J]. Journal of Kunming Medical University, 2023, 44(5): 12-18. doi: 10.12259/j.issn.2095-610X.S20230530

Citation:

Shaoyou DENG, Yulan ZHAO, Peijin WANG, Rong LI, Jintao LI, Hong ZHENG. Effects of Osteoking Combined with Antibiotic Cocktail on Insulin Resistance and Gut Flora in db/db Mice[J]. Journal of Kunming Medical University, 2023, 44(5): 12-18. doi: 10.12259/j.issn.2095-610X.S20230530

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Effects of Osteoking Combined with Antibiotic Cocktail on Insulin Resistance and Gut Flora in db/db Mice

doi: 10.12259/j.issn.2095-610X.S20230530

1.
Licensed Pharmacist Registration Center of Yunnan Province，Kunming Yunnan 650101
2.
Dept. of Laboratory Animal Science，Kunming Medial University， Kunming Yunnan 650500，China

Received Date: 2023-02-09
Publish Date: 2023-05-25

Abstract

Abstract

Objective To investigate the effects of Osteoking combined with antibiotic cocktail on insulin resistance and gut microbiota in db/db mice. Methods The wild mice were used as the control group, while db/db mice were randomly divided into the model group, Osteoking group （OK）, antibiotic group （ABX）, and Osteoking combined with antibiotic cocktail group （OA）. After the intragastric administration for 11 weeks, the following indices were investigated: body weight, fasting blood-glucose （FBG）, serum insulin and the changes of intestinal microflora by 16S rDNA sequencing technology in mice. Results Compared with the model group, levels of FBG and HOMA- IR were significantly decreased in OK, ABX, AO groups （P < 0.05）. And the shannon、simpson and pielou_e index of gut microbiota were significantly decreased in ABX group （ P < 0.05）, while they were increased in OK group （ P < 0.05）. The shannon and pielou_e index were increased in AO group （ P < 0.05）. At the phylum level, the relative abundance （RA） of Firmicutes （ P < 0.05） in OK group, RA of Proteobacteria in ABX （ P < 0.01）and AO groups （ P < 0.01） were significantly increased; while RA of Bacteroidota was significantly decreased in OK （ P < 0.05）, ABX （ P < 0.01）, AO （ P < 0.01）groups. At the family level, RA of Lachnospiraceae （ P < 0.05） in OK group, Enterobacteriaceae, Sutterellaceae in ABX and AO groups （ P <0.01）and Tannerellaceae in AO groups （ P < 0.01） were significantly increased; while RA of Muribaculaceae in OK group was significantly decreased （ P < 0.05）. The following gut microbiota were riched: Proteobacteria in ABX group, Lachnospiraceae NK4A136 group, Lactobacillus, Desulfobacteraceae and Prevotellaceae in OK group, Parabacteroides gordonii, Akkermansia, Morganellaceae and Alphaproteobacteria in AO group. Conclusion Osteoking, antibiotic cocktail alone or in combination have the effects of improving the insulin resistance in db/db mice. And Osteoking can improve the intestinal microflora imbalance induced by antibiotic cocktail.
- Antibiotic cocktail,
- Osteoking,
- Insulin resist,
- db/db mice,
- Gut microbiota

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References(23)

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