Effects of Osteoking Combined with Antibiotic Cocktail on Insulin Resistance and Gut Flora in db/db Mice

Shaoyou DENG; Yulan ZHAO; Peijin WANG; Rong LI; Jintao LI; Hong ZHENG

doi:10.12259/j.issn.2095-610X.S20230530

Volume 44 Issue 5

May 2023

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Shaoyou DENG, Yulan ZHAO, Peijin WANG, Rong LI, Jintao LI, Hong ZHENG. Effects of Osteoking Combined with Antibiotic Cocktail on Insulin Resistance and Gut Flora in db/db Mice[J]. Journal of Kunming Medical University, 2023, 44(5): 12-18. doi: 10.12259/j.issn.2095-610X.S20230530

Citation:

Shaoyou DENG, Yulan ZHAO, Peijin WANG, Rong LI, Jintao LI, Hong ZHENG. Effects of Osteoking Combined with Antibiotic Cocktail on Insulin Resistance and Gut Flora in db/db Mice[J]. Journal of Kunming Medical University, 2023, 44(5): 12-18. doi: 10.12259/j.issn.2095-610X.S20230530

Citation:

Shaoyou DENG, Yulan ZHAO, Peijin WANG, Rong LI, Jintao LI, Hong ZHENG. Effects of Osteoking Combined with Antibiotic Cocktail on Insulin Resistance and Gut Flora in db/db Mice[J]. Journal of Kunming Medical University, 2023, 44(5): 12-18. doi: 10.12259/j.issn.2095-610X.S20230530

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Effects of Osteoking Combined with Antibiotic Cocktail on Insulin Resistance and Gut Flora in db/db Mice

doi: 10.12259/j.issn.2095-610X.S20230530

1.
Licensed Pharmacist Registration Center of Yunnan Province，Kunming Yunnan 650101
2.
Dept. of Laboratory Animal Science，Kunming Medial University， Kunming Yunnan 650500，China

Received Date: 2023-02-09
Publish Date: 2023-05-25

Abstract

Abstract

Objective To investigate the effects of Osteoking combined with antibiotic cocktail on insulin resistance and gut microbiota in db/db mice. Methods The wild mice were used as the control group, while db/db mice were randomly divided into the model group, Osteoking group （OK）, antibiotic group （ABX）, and Osteoking combined with antibiotic cocktail group （OA）. After the intragastric administration for 11 weeks, the following indices were investigated: body weight, fasting blood-glucose （FBG）, serum insulin and the changes of intestinal microflora by 16S rDNA sequencing technology in mice. Results Compared with the model group, levels of FBG and HOMA- IR were significantly decreased in OK, ABX, AO groups （P < 0.05）. And the shannon、simpson and pielou_e index of gut microbiota were significantly decreased in ABX group （ P < 0.05）, while they were increased in OK group （ P < 0.05）. The shannon and pielou_e index were increased in AO group （ P < 0.05）. At the phylum level, the relative abundance （RA） of Firmicutes （ P < 0.05） in OK group, RA of Proteobacteria in ABX （ P < 0.01）and AO groups （ P < 0.01） were significantly increased; while RA of Bacteroidota was significantly decreased in OK （ P < 0.05）, ABX （ P < 0.01）, AO （ P < 0.01）groups. At the family level, RA of Lachnospiraceae （ P < 0.05） in OK group, Enterobacteriaceae, Sutterellaceae in ABX and AO groups （ P <0.01）and Tannerellaceae in AO groups （ P < 0.01） were significantly increased; while RA of Muribaculaceae in OK group was significantly decreased （ P < 0.05）. The following gut microbiota were riched: Proteobacteria in ABX group, Lachnospiraceae NK4A136 group, Lactobacillus, Desulfobacteraceae and Prevotellaceae in OK group, Parabacteroides gordonii, Akkermansia, Morganellaceae and Alphaproteobacteria in AO group. Conclusion Osteoking, antibiotic cocktail alone or in combination have the effects of improving the insulin resistance in db/db mice. And Osteoking can improve the intestinal microflora imbalance induced by antibiotic cocktail.
- Antibiotic cocktail,
- Osteoking,
- Insulin resist,
- db/db mice,
- Gut microbiota

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References(23)

References

[1]	Que Y Y,Cao M,He J Q,et al. Gut bacterial characteristics of patients with type 2 diabetes mellitus and the application potential[J]. Frontiers in Immunology,2021,12(6):722206-722217.
[2]	Schwalm N D,Groisman E A. Navigating the gut buffet: Control of polysaccharide utilization in bacteroides spp[J]. Trends Microbiol,2017,25(12):1005-1015. doi: 10.1016/j.tim.2017.06.009
[3]	杨艺. 恒古骨伤愈合剂对2型糖尿病性骨质疏松症的作用及机制研究 [D]. 昆明: 昆明医科大学硕士学位论文, 2022.
[4]	Ubeda C,Pamer E G. Antibiotics,microbiota,and immune defense[J]. Trends Immunol,2012,33(9):459-466. doi: 10.1016/j.it.2012.05.003
[5]	宋雪梅,金小琴,秦合伟,等. 中医药通过肠道菌群治疗 2 型糖尿病的进展[J]. 世界科学技术-中医药现代化,2023,25(1):1-12.
[6]	Page M M,Johnson J D. Mild suppression of hyperinsulinemia to treat obesity and insulin resistance[J]. Trends in Tndocrinology and Metabolism,2018,29(6):389-399.
[7]	Ianro G,Tilg H,Gasbarrini A. Antibiotics as deep modulators of gut microbiota: Between good and evil[J]. Gut,2016,65(11):1906-1915.
[8]	Rodrigues R R,Greer R L,Dong X,et al. Antibiotic-induced alterations in gut microbiota are associated with changes in glucose metabolism in healthy mice[J]. Front Microbiol,2017,8(2):2306-2320.
[9]	吴思谋,苗钟化,周青青,等. 长期抗生素暴露对高脂饮食负荷小鼠糖代谢和肠道菌群的影响[J]. 现代预防医学,2021,48(21):3878-3893.
[10]	Hansen C H,Krych L,Nielsen D S,et al. Early life treatment with vancomycin propagates Akkermansia muciniphila and reduces diabetes incidence in the NOD mouse[J]. Diabetologia,2012,5(3):2285-2294.
[11]	Fu L H,Qiu Y X,Shen L Y,et al. The delayed effects of antibiotics in type 2 diabetes,friend or foe?[J]. Journal of Endocrinology,2019,238(2):137-149.
[12]	Ma X,Brinker E,Graff E C,et al. Whole-genome shotgun metagenomic sequencing reveals distinct gut microbiome signatures of obese cats[J]. Microbiol Spectr,2022,10(3):837-852.
[13]	Ridaura V K,Faith J J,Rey F E,et al. Gut microbiota from twins discordant for obesity modulate metabolism in mice[J]. Science,2013(341):1241214-1241226.
[14]	Zhang J,Jin W,Jiang Y,et al. Response of milk performance,rumen and hindgut microbiome to dietary supplementation with aspergillus oryzae fermentation extracts in dairy cows[J]. Curr Microbiol,2022,79(4):113-125. doi: 10.1007/s00284-022-02790-z
[15]	Martin S A,Nisbet D J. Effect of direct-fed microbials on rumen microbial fermentation[J]. J Dairy Sci,1992,75(6):1736-1744. doi: 10.3168/jds.S0022-0302(92)77932-6
[16]	Wan Y,Li D. High-fat,low-carbohydrate diet was associated with unfavourable impact on colonic luminal microenvironment[J]. Gut,2020,69(9):1-13.
[17]	Kim Y I,Kim Y A,Kim H J,et al. Effect of helicobacter pylori treatment on the long-term mortality in patients with type 2 diabetes[J]. Korean J Intern Med,2021,36(3):584-595. doi: 10.3904/kjim.2019.428
[18]	Wang R,Yang X,Jiang Q,et al. Effect of mussel polysaccharide on glucolipid metabolism and intestinal flora in type 2 diabetic mice[J]. J Sci Food Agric,2023,103(7):3353-3366. doi: 10.1002/jsfa.12488
[19]	Wang K,Liao M F,Zhou N,et al. Parabacteroides distasonis alleviates obesity and metabolic dysfunctions via production of succinate and secondary bile acids[J]. Cell Rep,2019,26(1):222-235.e5. doi: 10.1016/j.celrep.2018.12.028
[20]	Bao L,Zhang Y,Zhang G,et al. Abnormal proliferation of gut mycobiota contributes to the aggravation of type 2 diabetes[J]. Commun Biol,2023,6(1):226-236. doi: 10.1038/s42003-023-04591-x
[21]	Xiao S,Liu C,Chen M,et al. Scutellariae radix and coptidis rhizoma ameliorate glycolipid metabolism of type 2 diabetic rats by modulating gut microbiota and its metabolites[J]. Appl Microbiol Biotechnol,2020,104(1):303-317. doi: 10.1007/s00253-019-10174-w
[22]	Martinez-Medina M,Denizot J,Dreux N,et al. Western diet induces dysbiosis with increased E coli in CEABAC10 mice,alters host barrier function favouring AIEC colonisation[J]. Gut,2014,63(1):116-124. doi: 10.1136/gutjnl-2012-304119
[23]	Gutierrez M W,Mercer E M,Moossavi S,et al. Maturational patterns of the infant microbiome are associated with early-life body mass index[J]. Cell Rep Med,2023,4(2):100928-100942. doi: 10.1016/j.xcrm.2023.100928

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