An Economical Method for Cultivation and Identification of Mast Cell Derived from Suckling Rabbit Bone Marrow

Yuxue WANG; Huali ZHANG; Lijun CHEN; Yongping LU

doi:10.12259/j.issn.2095-610X.S20230622

Volume 44 Issue 6

Jun. 2023

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Yuxue WANG, Huali ZHANG, Lijun CHEN, Yongping LU. An Economical Method for Cultivation and Identification of Mast Cell Derived from Suckling Rabbit Bone Marrow[J]. Journal of Kunming Medical University, 2023, 44(6): 1-5. doi: 10.12259/j.issn.2095-610X.S20230622

Citation:

Yuxue WANG, Huali ZHANG, Lijun CHEN, Yongping LU. An Economical Method for Cultivation and Identification of Mast Cell Derived from Suckling Rabbit Bone Marrow[J]. Journal of Kunming Medical University, 2023, 44(6): 1-5. doi: 10.12259/j.issn.2095-610X.S20230622

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An Economical Method for Cultivation and Identification of Mast Cell Derived from Suckling Rabbit Bone Marrow

doi: 10.12259/j.issn.2095-610X.S20230622

Dept. Ultrasonography，Affiliated Hospital of Yunnan University，Yunnan Kunming 650021，China

Received Date: 2023-03-06
Available Online: 2023-06-26
Publish Date: 2023-06-25

Abstract

Abstract

Objective To obtain more mature mast cells with more typical characteristics through a simple and well-operated in vitro induction culture system. Methods The bone marrow of suckling rabbits was blown out and put into high glucose medium containing DMEM, and the medium was changed every other day. When the cells were confluent, F12 induction medium was used, and the medium was changed every other day. When the cells became round under the microscope, 1640 maturation medium was replaced, and the medium was changed every 7 days until the cells were induced to mature. Toluidine blue staining was used to detect its function, and flow cytometry was used to detect the surface expression of CD117 and FcεR1α. Results After 6 weeks, toluidine blue staining showed that the cells containing more metachromatic particles could be obtained by adding β-mercaptoethanol. Flow cytometry showed that the proportion of CD117+ and FcεRIα+ double positive cells in the presence of β-mercapto ethanol was 96.2%, which was significantly higher than that in the absence of β-mercapto ethanol （P < 0.05）. Conclusion The use of SCF, IL-3 and β-mercaptoethanol can successfully induce mast cells derived from rabbit bone marrow, which provides foundation for the future research on inflammatory injury in rabbits, and makes subsequent basic and clinical research possible.
- Suckling rabbit,
- Bone marrow,
- Mast cells,
- Induction

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References(15)

References

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