Mechanism of Osteoking Improving Osteoarthritis based on Network Pharmacology and DDM Rats

Shaoyou DENG; Rong LI; Jintao LI; Yulan ZHAO; Peijin WANG; Hong ZHENG

doi:10.12259/j.issn.2095-610X.S20230701

Volume 44 Issue 7

Jul. 2023

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Article Navigation > Journal of Kunming Medical University > 2023 > 44(7): 34-39

Shaoyou DENG, Rong LI, Jintao LI, Yulan ZHAO, Peijin WANG, Hong ZHENG. Mechanism of Osteoking Improving Osteoarthritis based on Network Pharmacology and DDM Rats[J]. Journal of Kunming Medical University, 2023, 44(7): 34-39. doi: 10.12259/j.issn.2095-610X.S20230701

Citation:

Shaoyou DENG, Rong LI, Jintao LI, Yulan ZHAO, Peijin WANG, Hong ZHENG. Mechanism of Osteoking Improving Osteoarthritis based on Network Pharmacology and DDM Rats[J]. Journal of Kunming Medical University, 2023, 44(7): 34-39. doi: 10.12259/j.issn.2095-610X.S20230701

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Mechanism of Osteoking Improving Osteoarthritis based on Network Pharmacology and DDM Rats

doi: 10.12259/j.issn.2095-610X.S20230701

1.
Yunnan Certified Pharmacist Registration Center，Kunming Yunnan 650101
2.
Dept. of Laboratory Animal Science，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2023-03-27
Available Online: 2023-07-17
Publish Date: 2023-07-25

Abstract

Abstract

Objective To explore the potential mechanism of Osteoking （OK） in the treatment of Osteoarthritis （OA） and preliminarily verify it with DMM rats. Methods TCMSP database was used to collect potential targets of OK, GeneCards database and OMIM were used to screen target genes related to OA, and Cytoscape 3.8.2 software was used to establish a regulatory network for interaction between OK Active ingredient and OA target genes. The STRING database was used to construct protein interaction maps and GO and KECG were analyzed. In addition, OA rats were constructed using medial meniscus instability surgery （DMM） to observe knee joint cartilage lesions, and ELISA was used to detect inflammation and oxidative stress levels after OK treatment. Results Network pharmacology results showed that 80 core genes were involved in OK therapy for OA, including serum albumin （ALB） and interleukin-6 （IL6）, mainly involving biological processes such as immunity, inflammatory response, oxidative stress, and metabolism. Animal experiments showed that compared with the model group, the surface structure of the articular cartilage in the OK group of rats tended to be normal, with less detachment of chondrocytes; OK reduced serum inflammatory factors IL-6 （P < 0.01）, VEGF （P < 0.01）, and oxidative stress factor MDA （P < 0.05）, improved ALB levels and SOD activity （P < 0.01）. Conclusion OK can alleviate OA cartilage tissue damage, enhance immunity, and inhibit inflammation and oxidative stress reactions, preliminarily verifying the results of network pharmacology.
- Network pharmacology,
- Osteoking,
- Osteoarthritis,
- DMM rats

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References(24)

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