Mechanism of Osteoking Improving Osteoarthritis based on Network Pharmacology and DDM Rats

Shaoyou DENG; Rong LI; Jintao LI; Yulan ZHAO; Peijin WANG; Hong ZHENG

doi:10.12259/j.issn.2095-610X.S20230701

Volume 44 Issue 7

Jul. 2023

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2023 > 44(7): 34-39

Shaoyou DENG, Rong LI, Jintao LI, Yulan ZHAO, Peijin WANG, Hong ZHENG. Mechanism of Osteoking Improving Osteoarthritis based on Network Pharmacology and DDM Rats[J]. Journal of Kunming Medical University, 2023, 44(7): 34-39. doi: 10.12259/j.issn.2095-610X.S20230701

Citation:

Shaoyou DENG, Rong LI, Jintao LI, Yulan ZHAO, Peijin WANG, Hong ZHENG. Mechanism of Osteoking Improving Osteoarthritis based on Network Pharmacology and DDM Rats[J]. Journal of Kunming Medical University, 2023, 44(7): 34-39. doi: 10.12259/j.issn.2095-610X.S20230701

Citation:

PDF( 1653 KB)

Mechanism of Osteoking Improving Osteoarthritis based on Network Pharmacology and DDM Rats

doi: 10.12259/j.issn.2095-610X.S20230701

1.
Yunnan Certified Pharmacist Registration Center，Kunming Yunnan 650101
2.
Dept. of Laboratory Animal Science，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2023-03-27
Available Online: 2023-07-17
Publish Date: 2023-07-25

Abstract

Abstract

Objective To explore the potential mechanism of Osteoking （OK） in the treatment of Osteoarthritis （OA） and preliminarily verify it with DMM rats. Methods TCMSP database was used to collect potential targets of OK, GeneCards database and OMIM were used to screen target genes related to OA, and Cytoscape 3.8.2 software was used to establish a regulatory network for interaction between OK Active ingredient and OA target genes. The STRING database was used to construct protein interaction maps and GO and KECG were analyzed. In addition, OA rats were constructed using medial meniscus instability surgery （DMM） to observe knee joint cartilage lesions, and ELISA was used to detect inflammation and oxidative stress levels after OK treatment. Results Network pharmacology results showed that 80 core genes were involved in OK therapy for OA, including serum albumin （ALB） and interleukin-6 （IL6）, mainly involving biological processes such as immunity, inflammatory response, oxidative stress, and metabolism. Animal experiments showed that compared with the model group, the surface structure of the articular cartilage in the OK group of rats tended to be normal, with less detachment of chondrocytes; OK reduced serum inflammatory factors IL-6 （P < 0.01）, VEGF （P < 0.01）, and oxidative stress factor MDA （P < 0.05）, improved ALB levels and SOD activity （P < 0.01）. Conclusion OK can alleviate OA cartilage tissue damage, enhance immunity, and inhibit inflammation and oxidative stress reactions, preliminarily verifying the results of network pharmacology.
- Network pharmacology,
- Osteoking,
- Osteoarthritis,
- DMM rats

FullText(HTML)

References(24)

References

[1]	Li M H,Xiao R,Li J B,et al. Regenerative approaches for cartilage repair in the treatment of osteoarthritis[J]. Osteoarthritis and Cartilage,2017,25(10):1577-1587. doi: 10.1016/j.joca.2017.07.004
[2]	Wang P E,Zhang L,Ying J,et al. Bushenhuoxue formula attenuates cartilage degeneration in an osteoarthritic mouse model through TGF-β/MMP13 signaling[J]. Journal of Translational Medicine,2018,16(1):72-86. doi: 10.1186/s12967-018-1437-3
[3]	Wu G,Chen W,Fan H,et al. Duhuo jisheng decoction promotes chondrocyte proliferation through accelerated G1/S transition in osteoarthritis[J]. International Journal of Molecular Medicine,2013,32(5):1001-1013. doi: 10.3892/ijmm.2013.1481
[4]	Liu F,Liu G,Liang W,et al. Duhuo jisheng decoction treatment inhibits the sodium nitroprussiate-induced apoptosis of chondrocytes through the mitochondrial-dependent signaling pathway[J]. International Journal of Molecular Medicine,2014,34(6):1573-1580. doi: 10.3892/ijmm.2014.1962
[5]	吴继昆. 恒古骨伤愈合剂治疗157例骨关节炎疗效总结[J]. 中国现代药物应用,2018,12(24):196-197.
[6]	徐涛涛,郑凌歆,孙岩,等. 恒古骨伤愈合剂治疗膝骨性关节炎的临床疗效及安全性评价[J]. 中国中医骨伤科杂志,2022,30(2):8-12.
[7]	Ru J,Li P,Wang J,et al. TCMSP: A database of systems pharmacology for drug discovery from herbal medicines[J]. Journal of Cheminformatics,2014,6(3):13-25.
[8]	Szklarczyk D,Gable A L,Lyon D,et al. STRING v11: Protein-protein association networks with increased coverage,supporting functional discovery in genome-wide experimental datasets[J]. Nucleic Acids Research,2019,47(1):607-613.
[9]	Culley K L,Dragomir C L,Chang J,et al. Mouse models of osteoarthritis: surgical model of posttraumatic osteoarthritis induced by destabilization of the medial meniscus[J]. Methods in Molecular Biology (Clifton,NJ),2015,1226:143-173.
[10]	王庆甫,马玉峰,殷岳杉. 重新认识膝骨性关节炎的诊断和防治[J]. 中国骨伤,2016,29(9):779-781.
[11]	Lin Z,Fu C,Yan Z,et al. The protective effect of hesperetin in osteoarthritis: An in vitro and in vivo study[J]. Food & Function,2020,11(3):2654-2666.
[12]	Wang S N,Xie G P,Qin C H,et al. Aucubin prevents interleukin-1 beta induced inflammation and cartilage matrix degradation via inhibition of NF-κB signaling pathway in rat articular chondrocytes[J]. International Immunopharmacology,2015,24(2):408-415. doi: 10.1016/j.intimp.2014.12.029
[13]	Wang B W,Jiang Y,Yao Z L,et al. Aucubin protects chondrocytes against iL-1β-Induced apoptosis in vitro and inhibits osteoarthritis in mice model[J]. Drug Design,Development and Therapy,2019,13(9):3529-3538.
[14]	Ling H,Zeng Q,Ge Q,et al. Osteoking decelerates cartilage degeneration in DMM-Induced osteoarthritic mice model through TGF-β/smad-dependent manner[J]. Frontiers in Pharmacology,2021,12(2):678810-678823.
[15]	Kim S,Mcclave S A,Martindale R G,et al. Hypoalbuminemia and clinical outcomes: What is the mechanism behind the relationship?[J]. American Surgeon,2017,83(11):1220-1227. doi: 10.1177/000313481708301123
[16]	Zhang J,Wang T,Fang Y,et al. Clinical significance of serum albumin/globulin ratio in patients with pyogenic liver abscess[J]. Frontiers in Surgery,2021,8(3):677799-677813.
[17]	Nicholson J A,Dowrick A S,Liew S M. Nutritional status and short-term outcome of hip arthroplasty[J]. Journal of Orthopaedic Surgery,2012,20(3):331-345. doi: 10.1177/230949901202000313
[18]	耿秋东,张应生,卢文亚,等. 化湿定痛汤对木瓜蛋白酶诱导的膝骨关节炎大鼠血清IL-1、IL-6、IL-10的影响[J]. 中华中医药杂志,2020,35(9):4654-4657.
[19]	张磊,张劲. 滑膜炎膏治疗膝关节骨性关节炎疗效及对IL-1、IL-6水平影响的临床研究[J]. 湖北中医药大学学报,2020,22(2):41-44.
[20]	李飞龙,谢平金,柴生颋,等. 川芎嗪对膝骨性关节炎大鼠软骨VEGF表达的影响[J]. 中国骨质疏松杂志,2018,24(7):904-909.
[21]	徐慧,陆进明. VEGF在骨关节炎发病机制中的研究进展[J]. 安徽医学,2016,37(7):923-925.
[22]	Pfander D,Kortje D,Zimmermann R,et al. Vascular endothelial growth factor in articular cartilage of healthy and osteoarthritic human knee joints[J]. Annals of the Rheumatic Diseases,2001,60(11):1070-1083. doi: 10.1136/ard.60.11.1070
[23]	Nagao M,Hamilton J L,Kc R,et al. Vascular endothelial growth factor in cartilage development and osteoarthritis[J]. Scientific Reports,2017,7(1):13027-13039. doi: 10.1038/s41598-017-13417-w
[24]	Di Meo S,Venditti P. Evolution of the knowledge of free radicals and other oxidants[J]. Oxidative Medicine and Cellular Longevity,2020,2020:9829176-9829189.

Relative Articles(20)

Relative Articles

[1]	Yanhong YANG, Juan HE, Shan JIN, Piaopiao YE, Xin LI, Li QIAO. Prediction of Psoriasis and Potential Treatment of Traditional Chinese Medicine based on Reverse Network Pharmacology Analysis. Journal of Kunming Medical University, 2024, 46(): 1-9.
[2]	Feifei XIE, Yinzi XIN, Min XU, Jinghan LI, Wei WANG. Mechanisms of Long Non-Coding RNAs in Cartilage Development and Related Diseases. Journal of Kunming Medical University, 2024, 45(10): 1-7. doi: 10.12259/j.issn.2095-610X.S20241001
[3]	Yihan CHEN, Shanyong ZHANG, Yu DING, Li ZHANG. Research Progress on the Molecular Pathogenesis of Temporomandibular Joint Osteoarthritis. Journal of Kunming Medical University, 2024, 45(7): 1-5. doi: 10.12259/j.issn.2095-610X.S20240701
[4]	Hualei DAI, Chengcheng HU, Guimin ZHANG, Siming TAO, Jiankun CHEN. Screening Biomarkers of Astragalus Membranaceus for Hypertensive Ventricular Remodeling Based on Network Pharmacology and Molecular Docking. Journal of Kunming Medical University, 2023, 44(8): 27-36. doi: 10.12259/j.issn.2095-610X.S20230819
[5]	Shaoyou DENG, Yulan ZHAO, Peijin WANG, Rong LI, Jintao LI, Hong ZHENG. Effects of Osteoking Combined with Antibiotic Cocktail on Insulin Resistance and Gut Flora in db/db Mice. Journal of Kunming Medical University, 2023, 44(5): 12-18. doi: 10.12259/j.issn.2095-610X.S20230530
[6]	Wenli HUANGFU, Yao HUANG, Bo LIU, Changhai LV, Juan LIU, Zichao DAI. Comparison of Two Induction Methods of Temporo-mandibular Joint Osteoarthritis in Rat Models. Journal of Kunming Medical University, 2023, 44(3): 49-53. doi: 10.12259/j.issn.2095-610X.S20230308
[7]	Ranxi SHI, Tao YANG, Qing CHENG, Liangchen ZHAO, Jiahui GUO, Limei WANG. Mechanism of Dendrobium Officinale Against Inflammatory Aging Based on Network Pharmacology and Molecular Docking. Journal of Kunming Medical University, 2023, 44(11): 1-8. doi: 10.12259/j.issn.2095-610X.S20231101
[8]	Zihan WANG, Gaiying YE, Tao ZHAO, Jun ZHANG, Yu HU. Experimental Study on the Expression of mTOR in TMJOA Condylar Cartilage of SD Rats. Journal of Kunming Medical University, 2022, 43(10): 10-15. doi: 10.12259/j.issn.2095-610X.S20221033
[9]	Li ZHANG, Yuting WANG, Jianing SHI, Dan YU, Renhua YANG, Zhiqiang SHEN, Jiang LONG, Peng CHEN. Network Pharmacology-based Analysis of the Anti-atherosclerosis Mechanism of Scutellarin and Experimental Validation in Vivo. Journal of Kunming Medical University, 2022, 43(8): 17-27. doi: 10.12259/j.issn.2095-610X.S20220804
[10]	Yu-wei JI, Xin ZHAO, Jiang-li LU, Yi YANG, Wei TANG, Jian-lin JIAO. Effect of Osteoking on Bone Density and Bone Biomechanics of Tree Shrews in a Postmenopausal Osteoporotic Fracture Model. Journal of Kunming Medical University, 2020, 41(11): 7-11. doi: 10.12259/j.issn.2095-610X.S20201103
[11]	Yan Hui Ling , Zhao Hong Bin , Qian Chuan Yun , Wang Ying Zhong , Zhang Yang , Shen Xiang Lin , Jiang Gang , Gao Shuang . . Journal of Kunming Medical University, 2019, 40(01): 123-127.
[12]	Li Li , Li Jin Tao , Guo Yu Qing , Wu Chao , Lu Jiang Li , Zeng Yue Qin , Jiao Jian Lin . . Journal of Kunming Medical University, 2019, 40(06): 6-10.
[13]	Li Li , Zeng Yue Qin , Li Jin Tao , Zhao Hong Bin , Liang Zhang , Wang Li Mei . . Journal of Kunming Medical University, 2019, 40(07): 15-18.
[14]	Zheng Hong , Li Jin Tao , Yuan Xin , Wu Chao , Zeng He , Zhao Hong Bin , Tang Wei . Osteoking Promotes the Proliferation of BMSC through Activating TGF-β/Smad Signaling Pathway in Castrate Osteoporosis Rats. Journal of Kunming Medical University, 2018, 39(03): 5-10.
[15]	Yuan Xin , Zheng Hong , Jiao Jian Lin , Wu Chao , Zhao Hong Bin , Tang Wei . Effect of Osteoking on Blood Biochemical Indicator of Tree Shrew with Osteoporotic Fracture. Journal of Kunming Medical University, 2017, 38(07): 12-15.
[16]	Li Jin Tao , Zheng Hong , Li Bo , Yuan Xin , Tang Wei , Zhao Hong Bin . Effects of Osteoking on Estradiol and Bone Metabolism of Tree Shrews with Osteoporotic Fracture. Journal of Kunming Medical University, 2017, 38(11): 15-18.
[17]	Tang Tao , Sun Xian Run , Jiang Yan Ping , Zhang Min , Ma Hua , Long Yu . Correlation Between NO/iNOS and Destruction of Articular Cartilage. Journal of Kunming Medical University, 2016, 37(11): 28-32.
[18]	Dai Long Jin . . Journal of Kunming Medical University,
[19]	. Expression and Significance of ADAMTS-4 in Synovial Fluid of Knee Osteoarthritis. Journal of Kunming Medical University,
[20]	Wang Wei . . Journal of Kunming Medical University,