Value Evaluation of New Markers AFP-L3 and PIVKAⅡ in the Diagnosis of Liver Cancer

Liqiang LAN; Jing LI; Qingbo REN

doi:10.12259/j.issn.2095-610X.S20230705

Volume 44 Issue 7

Jul. 2023

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Article Navigation > Journal of Kunming Medical University > 2023 > 44(7): 136-141

Liqiang LAN, Jing LI, Qingbo REN. Value Evaluation of New Markers AFP-L3 and PIVKAⅡ in the Diagnosis of Liver Cancer[J]. Journal of Kunming Medical University, 2023, 44(7): 136-141. doi: 10.12259/j.issn.2095-610X.S20230705

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Liqiang LAN, Jing LI, Qingbo REN. Value Evaluation of New Markers AFP-L3 and PIVKAⅡ in the Diagnosis of Liver Cancer[J]. Journal of Kunming Medical University, 2023, 44(7): 136-141. doi: 10.12259/j.issn.2095-610X.S20230705

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Value Evaluation of New Markers AFP-L3 and PIVKAⅡ in the Diagnosis of Liver Cancer

doi: 10.12259/j.issn.2095-610X.S20230705

1.
Dept of General Internal Medicine
2.
Dept. of Clinical Laboratory，Qingdao Sixth People’s Hospital，Qingdao Shandong 266000，China

Received Date: 2023-03-28
Available Online: 2023-07-18
Publish Date: 2023-07-25

Abstract

Abstract

Objective To investigate the diagnostic value of AFP-L3 and PIVKAⅡ in liver cancer. Methods A total of 3066 patients with chronic liver disease who were admitted to Qingdao Sixth People’s Hospital from January 2019 to January 2022 were selected as objects of the research. The levels of AFP-L3, PIVKAⅡ, CK65, CK30, and TK1 in hepatitis, liver cirrhosis, and liver cancer were compared. The specificity and sensitivity were evaluated, and the ROC curve was used to evaluate the biomarkers and propose the optimal cutoff point. Results The expression of AFP-L3 and PIVKA in the liver cirrhosis group was higher than that in the hepatitis group （P < 0.0001）, while the expression of CK65, CK30, and TK in liver cirrhosis group was lower than that in the hepatitis group （P < 0.001）. The expression of AFP-L3, CK65, CK30 and PIVKA in the liver cancer group was significantly higher than that in the liver cirrhosis group （P < 0.0001）, while the expression of TK in the liver cancer group was lower than that in the liver cirrhosis group （P > 0.05）. Among the five biomarkers included in the study, AFP-L3 had the highest specificity （0.89） and PIVKAⅡ had the highest sensitivity （0.62）, and the combined application of AFP-L3 and PIVKAⅡ had a sensitivity of 0.70 and a specificity of 0.81, which was better than a single marker. The analysis of ROC curve showed that PIVKAⅡ had a higher diagnostic value （24）. Conclusion AFP-L3 is more specific than PIVKAⅡ in liver cancer screening, and the combination of AFP-L3 and PIVKAⅡ diagnosis may have diagnostic value in liver cancer.
- Liver cancer,
- Alpha-fetoprotein variants L3,
- Protein induced by vitamin K absence or antagonist II,
- Diagnostic value

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References(23)

References

[1]	Lee S E,Alcedo K P,Kim H J,et al. Alternative splicing in hepatocellular carcinoma[J]. Cell Mol Gastroenterol Hepatol,2020,10(4):699-712. doi: 10.1016/j.jcmgh.2020.04.018
[2]	Wallace M C,Preen D,Jeffrey G P,et al. The evolving epidemiology of hepatocellular carcinoma: A global perspective[J]. Expert Rev Gastroenterol Hepatol,2015,9(6):765-779. doi: 10.1586/17474124.2015.1028363
[3]	Anwanwan D,Singh S K,Singh S,et al. Challenges in liver cancer and possible treatment approaches[J]. Biochim Biophys Acta Rev Cancer,2020,1873(1):188314. doi: 10.1016/j.bbcan.2019.188314
[4]	Siegel R L,Miller K D,Fuchs H E,et al. Cancer statistics,2021[J]. CA Cancer J Clin,2021,71(1):7-33. doi: 10.3322/caac.21654
[5]	Daniele B, Bencivenga A, Megna A S, et al. Alpha-fetoprotein and ultrasonography screening for hepatocellular carcinoma[J]. Gastroenterology, 2004, 127（5 Suppl 1）: S108-S112.
[6]	Liu S,Wang M,Zheng C,et al. Diagnostic value of serum glypican-3 alone and in combination with AFP as an aid in the diagnosis of liver cancer[J]. Clin Biochem,2020,79:54-60. doi: 10.1016/j.clinbiochem.2020.02.009
[7]	Ibrahim H M,Elghannam M Z,Elkhawaga O a Y,et al. Evaluation of serum alpha fetoprotein-L3 as an accuracy novel biomarker for the early diagnosis of hepatocellular carcinoma in Egyptian patients[J]. Saudi J Biol Sci,2021,28(10):5760-5764. doi: 10.1016/j.sjbs.2021.06.020
[8]	Chaiteerakij R,Zhang X,Addissie B D,et al. Combinations of biomarkers and Milan criteria for predicting hepatocellular carcinoma recurrence after liver transplantation[J]. Liver Transpl,2015,21(5):599-606. doi: 10.1002/lt.24117
[9]	Liebman H A,Furie B C,Tong M J,et al. Des-gamma-carboxy (abnormal) prothrombin as a serum marker of primary hepatocellular carcinoma[J]. N Engl J Med,1984,310(22):1427-1431. doi: 10.1056/NEJM198405313102204
[10]	王贵强,王福生,庄辉,等. 慢性乙型肝炎防治指南(2019年版)[J]. 中国病毒病杂志,2020,10(1):1-25.
[11]	中国抗癌协会肝癌专业委员会. 中国肿瘤整合诊治指南(CACA)-肝癌部分[J]. 肿瘤综合治疗电子杂志,2022,8(3):31-63.
[12]	Wei W,Zeng H,Zheng R,et al. Cancer registration in China and its role in cancer prevention and control[J]. Lancet Oncol,2020,21(7):e342-e349. doi: 10.1016/S1470-2045(20)30073-5
[13]	Broadfield L A,Duarte J a G,Schmieder R,et al. Fat induces glucose metabolism in nontransformed liver cells and promotes liver tumorigenesis[J]. Cancer Res,2021,81(8):1988-2001. doi: 10.1158/0008-5472.CAN-20-1954
[14]	Franssen B,Jibara G,Tabrizian P,et al. Actual 10-year survival following hepatectomy for hepatocellular carcinoma[J]. HPB (Oxford),2014,16(9):830-835. doi: 10.1111/hpb.12206
[15]	Zhou J M,Wang T,Zhang K H. AFP-L3 for the diagnosis of early hepatocellular carcinoma: A meta-analysis[J]. Medicine (Baltimore),2021,100(43):e27673.
[16]	Chen Y,Luo C,Zhao M,et al. Administration of a PTEN inhibitor BPV(pic) attenuates early brain injury via modulating AMPA receptor subunits after subarachnoid hemorrhage in rats[J]. Neurosci Lett,2015,588:131-136. doi: 10.1016/j.neulet.2015.01.005
[17]	Li J,Gao T,Gu S,et al. An electrochemical biosensor for the assay of alpha-fetoprotein-L3 with practical applications[J]. Biosens Bioelectron,2017,87:352-357. doi: 10.1016/j.bios.2016.08.071
[18]	Wei T,Zhang W,Tan Q,et al. Electrochemical assay of the alpha fetoprotein-l3 isoform ratio to improve the diagnostic accuracy of hepatocellular carcinoma[J]. Anal Chem,2018,90(21):13051-13058. doi: 10.1021/acs.analchem.8b04045
[19]	Kotwani P,Chan W,Yao F,et al. DCP and AFP-L3 are complementary to AFP in predicting high-risk explant features: Results of a prospective study[J]. Clin Gastroenterol Hepatol,2022,20(3):701-703.e2. doi: 10.1016/j.cgh.2021.01.043
[20]	Fang Y S,Wu Q,Zhao H C,et al. Do combined assays of serum AFP,AFP-L3,DCP,GP73,and DKK-1 efficiently improve the clinical values of biomarkers in decision-making for hepatocellular carcinoma? A meta-analysis[J]. Expert Rev Gastroenterol Hepatol,2021,15(9):1065-1076. doi: 10.1080/17474124.2021.1900731
[21]	Abdel-Aziz M M,Elshal M F,Abass A T,et al. Comparison of AFP-L3 and p53 antigen concentration with alpha-fetoprotein as serum markers for hepatocellular carcinoma[J]. Clin Lab,2016,62(6):1121-1129.
[22]	Hadi H,Wan Shuaib W M A,Raja Ali R A,et al. Utility of PIVKA-II and AFP in differentiating hepatocellular carcinoma from non-malignant high-risk patients[J]. Medicina (Kaunas),2022,58(8):1015.
[23]	Sun X,Mei J,Lin W,et al. Reductions in AFP and PIVKA-II can predict the efficiency of anti-PD-1 immunotherapy in HCC patients[J]. BMC Cancer,2021,21(1):775. doi: 10.1186/s12885-021-08428-w

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