Application of Nanopore Sequencing in Tumor with Infection

Xi LIU; Xin LIU; Jingjing CAI; Yaxi DU; Hongsheng LI; Yongchun ZHOU

doi:10.12259/j.issn.2095-610X.S20230710

Volume 44 Issue 7

Jul. 2023

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Article Navigation > Journal of Kunming Medical University > 2023 > 44(7): 9-15

Xi LIU, Xin LIU, Jingjing CAI, Yaxi DU, Hongsheng LI, Yongchun ZHOU. Application of Nanopore Sequencing in Tumor with Infection[J]. Journal of Kunming Medical University, 2023, 44(7): 9-15. doi: 10.12259/j.issn.2095-610X.S20230710

Citation:

Xi LIU, Xin LIU, Jingjing CAI, Yaxi DU, Hongsheng LI, Yongchun ZHOU. Application of Nanopore Sequencing in Tumor with Infection[J]. Journal of Kunming Medical University, 2023, 44(7): 9-15. doi: 10.12259/j.issn.2095-610X.S20230710

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Application of Nanopore Sequencing in Tumor with Infection

doi: 10.12259/j.issn.2095-610X.S20230710

The Molecular Diagnosis Center，The 3rd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650118，China

Received Date: 2023-04-05
Available Online: 2023-07-18
Publish Date: 2023-07-25

Abstract

Abstract

Objective To evaluate the clinical application value of nanopore sequencing in tumor patients with concurrent infectious diseases and to provide a new detection method for rapid and sensitive diagnosis of infectious diseases in tumor patients. Methods Samples of inpatients with suspected infections from July to August 2022 at Yunnan Cancer Hospital were collected, and pathogenic microorganisms in the samples were identified using nanopore sequencing. Results Forty-six samples from 36 patients with suspected infection were collected. Nanopore sequencing detected 72.22% of bacteria, 31.48% of fungi, and 42.59% of viruses. Both bacterial and fungal detection rates were significantly higher （χ² = 23.19 , P < 0.0001; χ² = 13.14 , P = 0.0003） than the results by microbiological culture method （23.91% and 10.87%）. The nanopore sequencing results showed significantly more bacterial and fungal species detected compared to microbial culture results. Conclusions Nanopore sequencing for the identification of pathogenic microorganisms has the advantages of high detection rate, wide detection of pathogenic genera, and rapid diagnosis. It has potential clinical application in the diagnosis of tumor-associated infectious diseases.
- Nanopore sequencing,
- Tumor,
- Infection,
- Pathogenic microorganisms,
- Microbial culture

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References(22)

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