The Relationship between Arsenic Exposure and DNA Damage of EGFR，PTEN，Kras，and PIK3CA Genes in Arsenic Factory Workers

Jiang WU; He MA; Jinyun JIANG; Na LIU; Ruihuan ZHAO; Yuefeng HE

doi:10.12259/j.issn.2095-610X.S20230717

Volume 44 Issue 7

Jul. 2023

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2023 > 44(7): 29-33

Jiang WU, He MA, Jinyun JIANG, Na LIU, Ruihuan ZHAO, Yuefeng HE. The Relationship between Arsenic Exposure and DNA Damage of EGFR，PTEN，Kras，and PIK3CA Genes in Arsenic Factory Workers[J]. Journal of Kunming Medical University, 2023, 44(7): 29-33. doi: 10.12259/j.issn.2095-610X.S20230717

Citation:

Jiang WU, He MA, Jinyun JIANG, Na LIU, Ruihuan ZHAO, Yuefeng HE. The Relationship between Arsenic Exposure and DNA Damage of EGFR，PTEN，Kras，and PIK3CA Genes in Arsenic Factory Workers[J]. Journal of Kunming Medical University, 2023, 44(7): 29-33. doi: 10.12259/j.issn.2095-610X.S20230717

Citation:

Jiang WU, He MA, Jinyun JIANG, Na LIU, Ruihuan ZHAO, Yuefeng HE. The Relationship between Arsenic Exposure and DNA Damage of EGFR，PTEN，Kras，and PIK3CA Genes in Arsenic Factory Workers[J]. Journal of Kunming Medical University, 2023, 44(7): 29-33. doi: 10.12259/j.issn.2095-610X.S20230717

PDF( 609 KB)

The Relationship between Arsenic Exposure and DNA Damage of EGFR，PTEN，Kras，and PIK3CA Genes in Arsenic Factory Workers

doi: 10.12259/j.issn.2095-610X.S20230717

1.
Dept. of Physical Education，Kunming Medical University
2.
Key Laboratory of Public Health and Disease Control，Education Department of Yunnan Province，School of Public Health，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2023-06-01
Available Online: 2023-07-17
Publish Date: 2023-07-25

Abstract

Abstract

Objective To explore the relationship between DNA damage and urinary arsenic metabolism in four genes of EGFR, PTEN, Kras and PIK3CA in workers exposed to arsenic in arsenic factories. Methods A total of 78 first-line occupational arsenic exposure workers in an arsenic factory in Yunnan were selected as the exposure group and 24 people without history of arsenic exposure were included as the control group. The DNA damage of EGFR, PTEN, Kras and PIK3CA genes in peripheral blood lymphocytes was detected by real-time fluorescence quantitative PCR, and the contents of inorganic arsenic, monomethyl arsine acid and dimethylamine in the urine of all workers were detected and the percentages were calculated. Results The DNA damage and mean damage of the four genes in the occupational arsenic exposure group were significantly higher than those in the control group （P < 0.05）. The mean damage of the four genes was positively correlated with inorganic arsenic, monomethyl arsine acid, dimethylamine acid, and the percentage of monomethyl arsine in urine and negatively correlated with the percentage of dimethylamine （P < 0.05）. Conclusion The damage of important genes caused by arsenic exposure and the average damage of four genes have the potential to be used as markers of damage assessment.
- Arsenic,
- EGFR,
- PTEN,
- Kras,
- PIK3CA

FullText(HTML)

References(18)

References

[1]	Nigra A E,Moon K A,Jones M R,et al. Urinary arsenic and heart disease mortality in NHANES 2003–2014[J]. Environmental Research,2021,200:111387. doi: 10.1016/j.envres.2021.111387
[2]	Palma-Lara I,Martínez-Castillo M,Quintana-Pérez J C,et al. Arsenic exposure: A public health problem leading to several cancers[J]. Regulatory Toxicology and Pharmacology,2020,110:104539. doi: 10.1016/j.yrtph.2019.104539
[3]	Rahaman M S,Rahman M M,Mise N,et al. Environmental arsenic exposure and its contribution to human diseases,toxicity mechanism and management[J]. Environmental Pollution,2021,289:117940. doi: 10.1016/j.envpol.2021.117940
[4]	苏敏静,韩建文. 慢性砷暴露与DNA甲基化研究进展[J]. 中华地方病学杂志,2022,41(1):6.
[5]	Zhang Q,Li Y,Liu J,et al. Differences of urinary arsenic metabolites and methylation capacity between individuals with and without skin lesions in Inner Mongolia,Northern China[J]. Int J Environ Res Public Health,2014,11(7):7319-7332. doi: 10.3390/ijerph110707319
[6]	Gao S,Mostofa M G,Quamruzzaman Q,et al. Gene-environment interaction and maternal arsenic methylation efficiency during pregnancy[J]. Environment International,2019,125:43-50. doi: 10.1016/j.envint.2019.01.042
[7]	Chernoff M,Tong L,Demanelis K,et al. Genetic determinants of reduced arsenic metabolism efficiency in the 10q24.32 region are associated with reduced AS3MT expression in multiple human tissue types[J]. Toxicological Sciences,2020,176(2):382-395. doi: 10.1093/toxsci/kfaa075
[8]	Hu C,Leche CA 2^nd,Kiyatkin A,et al. Glioblastoma mutations alter EGFR dimer structure to prevent ligand bias[J]. Nature,2022,602(7897):518-522. doi: 10.1038/s41586-021-04393-3
[9]	Yehia L,Keel E,Eng C. The clinical spectrum of PTEN mutations[J]. Annual Review of Medicine,2020,71(1):103-116. doi: 10.1146/annurev-med-052218-125823
[10]	Judd J,Abdel Karim N,Khan H,et al. Characterization of KRAS mutation subtypes in non-small cell lung cancer[J]. Molecular Cancer Therapeutics,2021,20(12):2577-2584. doi: 10.1158/1535-7163.MCT-21-0201
[11]	Chong X,Chen J,Zheng N,et al. PIK3CA mutations-mediated downregulation of circLHFPL2 inhibits colorectal cancer progression via upregulating PTEN[J]. Mol Cancer,2022,21(1):118. doi: 10.1186/s12943-022-01531-x
[12]	He Y,Zhang R,Chen J,et al. The ability of arsenic metabolism affected the expression of lncRNA PANDAR,DNA damage,or DNA methylation in peripheral blood lymphocytes of laborers[J]. Hum Exp Toxicol,2020,39(5):605-613. doi: 10.1177/0960327119897101
[13]	Wen W,Wen J,Lu L,et al. Metabolites of arsenic and increased DNA damage of p53 gene in arsenic plant workers[J]. Toxicol Appl Pharmacol,2011,254(1):41-47. doi: 10.1016/j.taap.2011.04.013
[14]	Wang Q, Ma L, Sun B, et al. Reduced peripheral blood mitochondrial DNA copy number as identification biomarker of suspected arsenic-induced liver damage[J]. Biological Trace Element Research, 2023, Online ahead of print.
[15]	Qian Q,Chen Y,Wang J Q,et al. Use of the alkaline comet assay for monitoring genotoxic effects of arsenic in human populations[J]. Mutat Res Genet Toxicol Environ Mutagen,2021,867:503368. doi: 10.1016/j.mrgentox.2021.503368
[16]	Annangi B,Bonassi S,Marcos R,et al. Biomonitoring of humans exposed to arsenic,chromium, nickel,vanadium,and complex mixtures of metals by using the micronucleus test in lymphocytes[J]. Mutat Res Rev Mutat Res,2016,770(Pt A):140-161.
[17]	Ni G,Tan J,Wang M,et al. Polymorphisms of the AS3MT gene are associated with arsenic methylation capacity and damage to the P21 gene in arsenic trioxide plant workers[J]. Toxicol Ind Health,2021,37(12):727-736.
[18]	Boonma T,Navasumrit P,Parnlob V,et al. SAM and folic acid prevent arsenic-induced oxidative and nitrative DNA damage in human lymphoblast cells by modulating expression of inflammatory and DNA repair genes[J]. Chemico-Biological Interactions,2022,361:109965.

Relative Articles(20)

Relative Articles

[1]	Shengxi YANG, Jiade ZHU, Qiurui LI, Wenlian TU. Clinical Study of Aumolertinib Versus Osimertinib in the Treatment of EGFR-Mutated Advanced Non-Small Cell Lung Cancer. Journal of Kunming Medical University, 2024, 46(): 1-7.
[2]	Ni GUO, Cheng ZHANG, Chao HONG, Weipeng LIU, Yufeng YAO, Zhiling YAN. Correlation of KRAS Gene 3′ UTR Polymorphisms with Cervical Cancer and Cervical Intraepithelial Neoplasia in Chinese Han Population in Yunnan Province. Journal of Kunming Medical University, 2024, 45(2): 14-22. doi: 10.12259/j.issn.2095-610X.S20240203
[3]	Yan LIANG, Lei WANG, Ming LEI, Benchao CHEN, Ping SUN, Shuai LI, Li LIU, Qianrong WANG, Manlin LIAO, Qianli MA. Correlation between KRAS Gene Polymorphism and Non-small Cell Lung Cancer in Yunnan Han Population. Journal of Kunming Medical University, 2023, 44(2): 52-60. doi: 10.12259/j.issn.2095-610X.S20230210
[4]	Haimei GOU, Li FANG, Xiaowu ZHONG. Relationship between Mutations of KRAS，NRAS and BRAF Genes and Colorectal Liver Metastases. Journal of Kunming Medical University, 2023, 44(1): 19-24. doi: 10.12259/j.issn.2095-610X.S20230106
[5]	Maofang WU, Yongchun ZHOU, Jingjing CAI, Xin MO, Yinwei LI, Jiahui MAO. EGFR Gene Mutation State and Its Clinical Significance in Multi-nodular Lung Adenocarcinoma in Yunnan Province. Journal of Kunming Medical University, 2022, 43(2): 60-66. doi: 10.12259/j.issn.2095-610X.S20220215
[6]	Zhong FANG, Xiao-dong XU, Li-hua WANG, Ying ZHANG, Chao MA. Prognostic Value of eGFR and NT-proBNP in Patients with Chronic Heart Failure Complicated with Atrial Fibrillation. Journal of Kunming Medical University, 2021, 42(11): 111-116. doi: 10.12259/j.issn.2095-610X.S20211120
[7]	Jian-wei SUN, Qian XIANG, Zi-chao LIU, Shan-shan XU, Ding DING. Effect of PTEN Gene Expression on Apoptosis of Thyroid Cancer Cells BCPAP and FTC133 and Expression of ERK and AKT. Journal of Kunming Medical University, 2021, 42(8): 23-30. doi: 10.12259/j.issn.2095-610X.S20210805
[8]	Ying-xia WANG, Qin HE, Guo-fang WANG, Xuan ZHANG. Expression and Significance of microRNA-21 and EGFR in Non-small Cell Lung Cancer. Journal of Kunming Medical University, 2021, 42(8): 101-105. doi: 10.12259/j.issn.2095-610X.S20210818
[9]	Wang Juan , Su Guo Miao , Pan Guo Qing , Bian Li , Yang Zhe , Zeng Ding Tao . . Journal of Kunming Medical University, 2020, 41(09): 1-6.
[10]	Sun Jian Wei , Wang Jian Song , Liu Zi Chao , Zheng Li Min . . Journal of Kunming Medical University, 2020, 41(01): 18-25.
[11]	Lu Xiao Dong , Lu Fang Li , Duan Zhao , Du Lian Jiang . Significance of Thyroid Transcription Factor-1 and Phosphatase and Tensin Homolog Genes Expression in Early Endometrial Cancer. Journal of Kunming Medical University, 2017, 38(08): 62-66.
[12]	Qian Lu , Bian Li . Research Progress of EGFR Mutations in Lung Cancer and Targeted Drug Resistance Mechanisms. Journal of Kunming Medical University, 2017, 38(08): 126-129.
[13]	Hu Yu Chong , Lu Jing Kun , Cui Meng Yao . . Journal of Kunming Medical University, 2017, 38(03): 27-30.
[14]	Li Zheng . The Diagnosis of Intrahepatic Bile Duct Stones with Early Intrahepatic Cholangiocarcinoma by Combining CA199, CEA, CA50 and ALP and Magnetic Resonance Imaging. Journal of Kunming Medical University,
[15]	Zhong Fang Fang . . Journal of Kunming Medical University,
[16]	Yan Ting . . Journal of Kunming Medical University,
[17]	Sun Jian Wei . . Journal of Kunming Medical University,
[18]	Zhao Chun Fang . . Journal of Kunming Medical University, 2013, 34(01): 1-1.
[19]	Han Rui Ping . Surveillance of Arsenic Pollution in Yangzonghai Lake in Recent Three Years. Journal of Kunming Medical University,
[20]	. The Expressions and Significance of ADAM10,EGFR and E-cadherin in Hepatocellular Carcinoma. Journal of Kunming Medical University,