Effects of Euonymine on Proliferation，Migration，and Cell Cycle of HUVECs and VSMCs

Li ZHANG; Xi ZHAO; Huaxiang ZHAO; Bao XIONG; Jie XU; Guangping TANG; Zhuo YU; Peng CHEN

doi:10.12259/j.issn.2095-610X.S20230723

Volume 44 Issue 7

Jul. 2023

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Article Navigation > Journal of Kunming Medical University > 2023 > 44(7): 1-8

Li ZHANG, Xi ZHAO, Huaxiang ZHAO, Bao XIONG, Jie XU, Guangping TANG, Zhuo YU, Peng CHEN. Effects of Euonymine on Proliferation，Migration，and Cell Cycle of HUVECs and VSMCs[J]. Journal of Kunming Medical University, 2023, 44(7): 1-8. doi: 10.12259/j.issn.2095-610X.S20230723

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Li ZHANG, Xi ZHAO, Huaxiang ZHAO, Bao XIONG, Jie XU, Guangping TANG, Zhuo YU, Peng CHEN. Effects of Euonymine on Proliferation，Migration，and Cell Cycle of HUVECs and VSMCs[J]. Journal of Kunming Medical University, 2023, 44(7): 1-8. doi: 10.12259/j.issn.2095-610X.S20230723

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Effects of Euonymine on Proliferation，Migration，and Cell Cycle of HUVECs and VSMCs

doi: 10.12259/j.issn.2095-610X.S20230723

Li ZHANG¹,
Xi ZHAO²,
Huaxiang ZHAO³,
Bao XIONG¹,
Jie XU¹,
Guangping TANG¹,
Zhuo YU^{4
,
,},
Peng CHEN^{1
,
,}

1.
School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products，Kunming Medical University，Kunming Yunan 650500
2.
Dept. of Cardiology，Ganmei Hospital of Kunming 1st People’s Hospital，Kunming Yunnan 650224
3.
Dept. of Cardiology，Wenshan People’s Hospital，Wenshan Yunnan 663007
4.
Dept. of Cardiology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2023-04-17
Available Online: 2023-07-17
Publish Date: 2023-07-25

Abstract

Abstract

Objective To investigate the effects of Euonymine on isolated cultured human umbilical vein endothelial cells （HUVECs） and human vascular smooth muscle cells （VSMCs）, so as to elucidate the possible mechanism of Euonymine in prevention and treatment of ISR. Methods The effect of trypan blue assay on the viability of HUVECs. MTT assay was used to observe the effect of Euonymine on the proliferation of normal HUVECs and VSMCs. A wound healing assay was performed to detect the effect of Euonymine on the migration of VSMCs. Results The MTT results showed that the absorbance of HUVECs and VSMCs decreased after the Euonymine intervention. In addition, the IC₅₀ of Euonymine on HUVECs after 24 hours and on VSMCs after 48 hours were 28.6 µg/mL and 33.92 µg/mL, respectively. Flow cytometry results showed that Euonymine （25 μg/mL） arrested the cell cycle of both HUVECs and VSMCs at the G₀/G₁ phase, while Euonymine （100 μg/mL） blocked the mitosis of VSMCs at both the G₀/G₁ and G₂/M phases. Conclusion Euonymine affects the cell cycle mainly by inhibiting the proliferation and migration of VSMCs and has potential value of clinical applications in inhibiting neointima hyperplasia after percutaneous coronary intervention （PCI）.
- Euonymine,
- HUVECs,
- VSMCs,
- Anti-proliferative,
- Anti-migratory,
- Cell cycle

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References(29)

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