The Regulatory Mechanism of miR-153-3p on Lumbar Degeneration

Shan YU; Lin XIAO; Dongping GONG; Loufeng LIANG; Anwei LIANG; Xiayi XU; Jie LIN; Huaxin LIANG

doi:10.12259/j.issn.2095-610X.S20230807

Volume 44 Issue 8

Aug. 2023

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Shan YU, Lin XIAO, Dongping GONG, Loufeng LIANG, Anwei LIANG, Xiayi XU, Jie LIN, Huaxin LIANG. The Regulatory Mechanism of miR-153-3p on Lumbar Degeneration[J]. Journal of Kunming Medical University, 2023, 44(8): 53-58. doi: 10.12259/j.issn.2095-610X.S20230807

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The Regulatory Mechanism of miR-153-3p on Lumbar Degeneration

doi: 10.12259/j.issn.2095-610X.S20230807

Dept. of Pain Management，International Zhuang Medicine Hospital Affiliated to Guangxi University of Traditional Chinese Medicine，Nanning Guangxi 530200，China

Received Date: 2023-05-13
Available Online: 2023-09-06
Publish Date: 2023-08-30

Abstract

Abstract

objective To investigate the effect of H₂O₂ on lumbar nucleus pulposus cells and the regulatory mechanism of miR-153-3p on lumbar degeneration. Methods CCK8 was used to detect cell viability in control group and H₂O₂ group, and flow cytometry was used to detect reactive oxygen species and mitochondrial membrane potential in the two groups, and qPCR was used to detect the expression levels of matrix metalloproteinase 3 （MMP3）, Nrf2, miR-153-3p and type II collagen （COL-Ⅱ） in the above two groups. Results Compared with the control group, the H₂O₂-treated lumbar nucleus pulposus cells showed reduced viability and mitochondrial membrane potential. The expression levels of miR-153-3p, COL-Ⅱ, MMP3 and Nrf2 in the control group and the H₂O₂ group were detected by qPCR, and the results showed that there was no significant difference in Nrf2 expression levels in the control group and the H₂O₂ group （P < 0.05）. The expression levels of miR-153-3p and MMP3 in the H₂O₂ group were lower than those in the control group, while the expression of COL-Ⅱ was lower than that in the control group and there was a statistical difference （P < 0.05）. Western blot showed that the expression of Nrf2 in the control group, inhibitor-NC group and the inhibitor group was significantly higher than that in the control group and inhibitor-NC group （P < 0.05）. The expression of Nrf2 in the cytoplasm and nucleus of inhibitor-NC group was not significantly different from that of control group （P > 0.05）. Conclusion Inhibition of miR-153-3p expression can up-regulate the expression level of Nrf2 in lumbar nucleus pulposus cells, suggesting that miR-153-3p's involvement in the process of LDD may be related to its regulation of Nrf2 expression and mitochondrial autophagy.
- lumbar degenerative diseases,
- Matrix metalloproteinase 3,
- Nrf2,
- miR-153-3p,
- Type Ⅱ collage （COL-Ⅱ）

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