Screening and Validation of Potential Prognostic Markers of Cervical Squamous Cell Carcinoma Based on The Cancer Genome Atlas Database

Yijun YAN; Hongying YANG; Hongping ZHANG; Zheng LI; Yue JIA; Min ZHAO

doi:10.12259/j.issn.2095-610X.S20230808

Volume 44 Issue 8

Aug. 2023

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Yijun YAN, Hongying YANG, Hongping ZHANG, Zheng LI, Yue JIA, Min ZHAO. Screening and Validation of Potential Prognostic Markers of Cervical Squamous Cell Carcinoma Based on The Cancer Genome Atlas Database[J]. Journal of Kunming Medical University, 2023, 44(8): 14-26. doi: 10.12259/j.issn.2095-610X.S20230808

Citation:

Yijun YAN, Hongying YANG, Hongping ZHANG, Zheng LI, Yue JIA, Min ZHAO. Screening and Validation of Potential Prognostic Markers of Cervical Squamous Cell Carcinoma Based on The Cancer Genome Atlas Database[J]. Journal of Kunming Medical University, 2023, 44(8): 14-26. doi: 10.12259/j.issn.2095-610X.S20230808

Citation:

Yijun YAN, Hongying YANG, Hongping ZHANG, Zheng LI, Yue JIA, Min ZHAO. Screening and Validation of Potential Prognostic Markers of Cervical Squamous Cell Carcinoma Based on The Cancer Genome Atlas Database[J]. Journal of Kunming Medical University, 2023, 44(8): 14-26. doi: 10.12259/j.issn.2095-610X.S20230808

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Screening and Validation of Potential Prognostic Markers of Cervical Squamous Cell Carcinoma Based on The Cancer Genome Atlas Database

doi: 10.12259/j.issn.2095-610X.S20230808

1.
Dept. of Nuclear Medicine，Affiliated Hospital of Yunnan University，Kunming Yunnan 650021
2.
Dept. of Gynecology
3.
Dept. of Medical Administration，The Third Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650118，China

Received Date: 2023-05-17
Available Online: 2023-09-01
Publish Date: 2023-08-30

Abstract

Abstract

Objective To screen and verify the potential prognostic markers of miRNA in cervical squamous cell carcinoma （CESC）, and to explore the molecular mechanism of CESC. Methods Using miRNA sequencing data of cervical squamous cell carcinoma tissues and adjacent tissues in the TCGA database, as well as patient clinical data, a risk model for predicting the prognosis of cervical squamous cell carcinoma patients was constructed using bioinformatics analysis. The model consists of four miRNAs （miR-505-5p, miR-142-3p, miR-532-5p, miR-218-1-3p）. The diagnostic performance of the model was evaluated. Reverse transcription-polymerase chain reaction （RT-PCR） was used to detect the expression levels of these four miRNAs in 21 pairs of cervical squamous cell carcinoma tissues and adjacent normal tissues. MiR-505-5p, which showed significant differential expression, was selected for further analysis to examine its relationship with various clinical pathological features of cervical squamous cell carcinoma patients. Transfection of miR-505-5p mimics into cervical squamous cell carcinoma cells was performed to investigate the impact of miR-505-5p on the cellular functions of cervical squamous cell carcinoma. Finally, potential target genes of miR-505-5p were predicted using bioinformatics methods and validated through RT-PCR and Western blot experiments. Results By analyzing the cervical squamous cell carcinoma （CESC） related data from the TCGA database, a prognostic risk model composed of 4 miRNAs （miR-505-5p, miR-142-3p, miR-532-5p, miR-218-1-3p） was constructed. The prognostic risk model effectively stratifies CESC patients into high-risk and low-risk groups for adverse prognosis. The expression of miR-505-5p in the collected 21 pairs of CESC tissues was lower than that in adjacent tissues （P < 0.05）, and its expression level was negatively correlated with the clinical stage and invasion degree of CESC patients. Overexpression of miR-505-5p inhibited the proliferation （P < 0.05） and migration of cervical squamous cell carcinoma SiHa cells, and promoted apoptosis （P < 0.05）. TBL1XR1 was identified as the best predicted target gene of miR-505-5p, and overexpression of miR-505-5p decreased the RNA and protein levels of TBL1XR1 （P < 0.05）. Conclusion miR-505-5p can be used as a potential prognostic marker in patients with CESC, and miR-505-5p may play a tumor suppressive role in CESC by targeting TBL1XR1.
- Cervical squamous cell carcinoma,
- Prognostic markers,
- miR-505-5p,
- TBL1XR1

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