miR-149-5p Regulates Malignant Biological Behavior of Glioma Cells Through MSH5/WNT Signaling Pathway

Yi FENG; Xiaofeng WANG; Ximin BAI; Sheng YAO; Juntao DANG; Yunjie ZHAO; Bing CAI

doi:10.12259/j.issn.2095-610X.S20230823

Volume 44 Issue 8

Aug. 2023

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Article Navigation > Journal of Kunming Medical University > 2023 > 44(8): 59-70

Yi FENG, Xiaofeng WANG, Ximin BAI, Sheng YAO, Juntao DANG, Yunjie ZHAO, Bing CAI. miR-149-5p Regulates Malignant Biological Behavior of Glioma Cells Through MSH5/WNT Signaling Pathway[J]. Journal of Kunming Medical University, 2023, 44(8): 59-70. doi: 10.12259/j.issn.2095-610X.S20230823

Citation:

Yi FENG, Xiaofeng WANG, Ximin BAI, Sheng YAO, Juntao DANG, Yunjie ZHAO, Bing CAI. miR-149-5p Regulates Malignant Biological Behavior of Glioma Cells Through MSH5/WNT Signaling Pathway[J]. Journal of Kunming Medical University, 2023, 44(8): 59-70. doi: 10.12259/j.issn.2095-610X.S20230823

Citation:

Yi FENG, Xiaofeng WANG, Ximin BAI, Sheng YAO, Juntao DANG, Yunjie ZHAO, Bing CAI. miR-149-5p Regulates Malignant Biological Behavior of Glioma Cells Through MSH5/WNT Signaling Pathway[J]. Journal of Kunming Medical University, 2023, 44(8): 59-70. doi: 10.12259/j.issn.2095-610X.S20230823

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miR-149-5p Regulates Malignant Biological Behavior of Glioma Cells Through MSH5/WNT Signaling Pathway

doi: 10.12259/j.issn.2095-610X.S20230823

1.
Dept. of Neurosurgery
2.
Dept. of Pathology，Weinan Central Hospital， Weinan Shanxi 714000，China

Received Date: 2023-06-13
Available Online: 2023-09-04
Publish Date: 2023-08-30

Abstract

Abstract

Objective To explore the mechanism of miR-149-5p regulating the malignant biological behavior of glioma cells through MSH5/Wnt signaling pathway. Methods RT-qPCR was used to detected the expression of miR-149-5p in glioma tissues and cell lines A172, U251, HS683 and H4. CCK-8 was used to detected cell viability, and EDU staining was performed to measure cell proliferation, Transwell assay was used to detected cell migration and invasion, and flow cytometry examined cell cycle and apoptosis. The dual luciferase reporter gene experiment verified the targeting relationship between miR-149-5p and MSH5. The protein expressions of MSH5, GAPDH, GSK3β, β-catenin and AXIN2 were detected by Western blot. Results The expression of miR-149-5p was decreased in glioma tissues （P < 0.0001） and cell lines （P < 0.0001）. Overexpression of miR-149-5p significantly inhibited the proliferation （P < 0.01）, migration （P < 0.05）, invasion （P < 0.01）, and cell cycle （P < 0.01） of A172 cells, and induced cell apoptosis （P < 0.01）. However, knocking down miR-149-5p had the opposite effect. Dual luciferase assay verified that miR-149-5p targeted MSH5. Knockdown of miR-149-5p reversed the effect of knocking down MSH5 on the malignant biological behavior of A172 cells. Overexpression of MSH5 promoted the proliferation, migration and invasion of A172 cells, cell cycle, and inhibited apoptosis by inhibiting GSK3β and activating β-catenin/AXIN2 pathway. Conclusions miR-149-5p inhibited the AXIN2 expression and activated the β-catenin/AXIN2 pathway by targeting up-regulation of MSH5, thereby inhibited the malignant biological behavior of stromal tumor cells.
- Glioma,
- miR-149-5p,
- MutS Homolog 5,
- Malignant biological behavior,
- Proliferation,
- Metastasis

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References(23)

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