The Antinociception of Galanin on Nucleus Accumbens and Its Mechanism in Rats

Yuhang PENG; Yanjuan LI; Jinghong QU; Daihuan TANG; Hongwei ZHANG; Rui WU; Shilian XU

doi:10.12259/j.issn.2095-610X.S20230925

Volume 44 Issue 9

Sep. 2023

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Yuhang PENG, Yanjuan LI, Jinghong QU, Daihuan TANG, Hongwei ZHANG, Rui WU, Shilian XU. The Antinociception of Galanin on Nucleus Accumbens and Its Mechanism in Rats[J]. Journal of Kunming Medical University, 2023, 44(9): 1-6. doi: 10.12259/j.issn.2095-610X.S20230925

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Yuhang PENG, Yanjuan LI, Jinghong QU, Daihuan TANG, Hongwei ZHANG, Rui WU, Shilian XU. The Antinociception of Galanin on Nucleus Accumbens and Its Mechanism in Rats[J]. Journal of Kunming Medical University, 2023, 44(9): 1-6. doi: 10.12259/j.issn.2095-610X.S20230925

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The Antinociception of Galanin on Nucleus Accumbens and Its Mechanism in Rats

doi: 10.12259/j.issn.2095-610X.S20230925

Dept. of Physiology，Faculty of Basic Medical Science，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2023-04-01
Available Online: 2023-09-23
Publish Date: 2023-09-30

Abstract

Abstract

Objective To research the analgesic effect of galanin on native rats, inflammatory rats and neuropathic rats in nucleus accumbens （NAc）, and to explore its mechanism. Methods A inflammatory pain model was prepared by subcutaneous injection of carrageine into rat’s plantar, while the model of neuralgia was prepared by ligation of the left sciatic nerve. The hind-paw withdraw latency （HWL） induced by thermal stimulation and the hind-paw withdraw threshold （HWT） induced by mechanical stimulation were measured to reflect the pain threshold. The expression of galanin receptors （GalRs） in the nucleus accumbens of inflammatory and neuropathic rats were detected by western blot （WB）. Results 1. The HWL of normal （P_left < 0.05; P_right < 0.01）, inflammatory （P_left < 0.001; P_right < 0.001） and neuropathic （P_left < 0.001; P_right < 0.001） rats were increased by injection of galanin into nucleus accumbens, and the HWT of normal （P_left < 0.001; P_right < 0.01）, inflammatory （P_left < 0.001; P_right < 0.001） and neuropathic （P_left < 0.001; P_right < 0.001） rats also were increased. 2. The antinociception of galanin on the rats with inflammatory （HWL: P_left < 0.05, P_right < 0.05; HWT: P_left < 0.01, P_right < 0.05） or neuropathic pain （HWL: P_left < 0.05, P_right < 0.05; HWT: P_left < 0.001, P_right < 0.001） was stronger than that of the native rats. However, the antinociception of galanin on the rats with the inflammatory pain and neuropathic pain was not significantly different （P > 0.05）. 3. The expressions of GalR1 were increased in rats after 3 hours of subcutaneous injection of carrageine （P < 0.001） and 14 days of sciatic nerve ligation （P < 0.001）, and the expressions of GalR2 also were increased in rats after 3 hours of subcutaneous injection of carrageine （P < 0.05） and 14 days of sciatic nerve ligation （P < 0.05）. Conclusion Intra-NAc injection of galanin has the antinociceptive effects on native, inflammatory and neuralgia rats and the antinociceptive effect of galanin in rats is induced by the activation of GalRs.
- Galanin,
- Neuralgia,
- Inflammatory pain,
- Antinociceptive effect,
- Nucleus accumbens

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[1]	Tatemoto K,Rökaeus A,Jörnvall H,et al. Galanin-a novel biologically active peptide from porcine intestine[J]. FEBS Lett,1983,164(1):124-128. doi: 10.1016/0014-5793(83)80033-7
[2]	Taylor T S,Konda P,John S S,et al. Galanin suppresses visceral afferent responses to noxious mechanical and inflammatory stimuli[J]. Physiological Reports,2020,8(2):e14326.
[3]	Yu M,Fang P H,Wang H,et al. Beneficial effects of galanin system on diabetic peripheral neuropathic pain and its complications[J]. Peptides,2020,134(12):170404.
[4]	Liu F, Yajima T, Wang M, et al. Effects of trigeminal nerve injury on the expression of galanin and its receptors in the rat trigeminal ganglion [J]. Neuropeptides, 2020, 84（期号!）: 102098. Liu F,Yajima T,Wang M,et al. Effects of trigeminal nerve injury on the expression of galanin and its receptors in the rat trigeminal ganglion[J]. Neuropeptides,2020,84:102098.
[5]	Amorim D,David-Pereira A,Marques P,et al. A role of supraspinal galanin in behavioural hyperalgesia in the rat[J]. Plos one,2014,9(11):e113077. doi: 10.1371/journal.pone.0113077
[6]	Dias E V,Sartori C R,Mariao P R,et al. Nucleus accumbens dopaminergic neurotransmission switches its modulatory action in chronification of inflammatory hyperalgesia[J]. Eur J Neurosci,2015,42(7):2380-2389. doi: 10.1111/ejn.13015
[7]	Duan H,Zhang Y,Zhang X M,et al. Antinociceptive roles of galanin receptor 1 in nucleus accumbens of rats in a model of neuropathic pain[J]. J Neurosci Res,2015,93(10):1542-1551. doi: 10.1002/jnr.23611
[8]	Mitsukawa K,Lu X,Bartfai T. Galanin,galanin receptors and drug targets[J]. Cell Mol Life Sci,2008,65(12):1796-1805. doi: 10.1007/s00018-008-8153-8
[9]	Gear R W,Aley K O,Levine J D. Pain—induced analgesia mediated by mesolimbic reward circuits[J]. J Neurosci,1999,19(16):7175-7181. doi: 10.1523/JNEUROSCI.19-16-07175.1999
[10]	Watanabe M,Sugiura Y,Sugiyama E,et al. Extracellular N- acetylaspartylglutamate released in the nucleus accumbens modulates the pain sensation: Analysis using a microdialysis/mass spectrometry integrated system[J]. Mol Pain,2018,14:1744806918754934.
[11]	Zhang Y, Gao Y, Li CY, et al. Galanin plays a role in antinociception via binding to galanin receptors in the nucleus accumbens of rats with neuropathic pain [J]. Neurosci Lett, 2019, 706（期号?）: 93-98. Zhang Y,Gao Y,Li C Y,et al. Galanin plays a role in antinociception via binding to galanin receptors in the nucleus accumbens of rats with neuropathic pain[J]. Neurosci Lett,2019,706:93-98.
[12]	Zhang, ML, Fu, FH, Yu, LC. Antinociception induced by galanin in anterior cingulate cortex in rats with acute inflammation [J]. Neurosci. Lett. 2017, 638（期号?）: 156-161. Zhang M L,Fu F H,Yu L C. Antinociception induced by galanin in anterior cingulate cortex in rats with acute inflammation[J]. Neurosci Lett,2017,638:156-161.
[13]	Zhang M L,Wang H B,Fu F H,et al. Involvement of galanin and galanin receptor 2 in nociceptive modulation in anterior cingulate cortex of normal rats and rats with mononeuropathy[J]. Sci Rep,2017,7(1):45930. doi: 10.1038/srep45930
[14]	Li S Y,Huo M L,Wu X Y,et al. Involvement of galanin and galanin receptor 1 in nociceptive modulation in the central nucleus of amygdala in normal and neuropathic rats[J]. Sci Rep,2017,7(1):15317. doi: 10.1038/s41598-017-13944-6
[15]	Li L X, Wang X B, Yu L C. Involvement of opioid receptors in the CGRP-induced antinociception in the nucleus accumbens of rats[J]. Brain Res,2010,1353:53-59.
[16]	Hobson S A,Bacon A,Elliot-Hunt C R,et al. Galanin acts as a trophic factor to the central and peripheral nervous systems[J]. Cell Mol Life Sci,2008,65(12):1806-1812.
[17]	Barreda-Gómez G,Giralt M T,Rodríguez-Puertas R. G protein-coupled galanin receptor distribution in the rat central nervous system[J]. Neuropeptides,2005,39(3):153-156.
[18]	Lang R,Gundlach A L,Holmes F E,et al. Physiology,signaling,and pharmacology of galanin peptides and receptors: Three decades of emerging diversity[J]. Pharmacol Rev,2015,67(1):118-175. doi: 10.1124/pr.112.006536
[19]	Chen S H,Lue J H,Hsiao Y J,et al. Elevated galanin receptor type 2 primarily contributes to mechanical hypersensitivity after median nerve injury[J]. PLoS ONE,2018,13(6):e0199512.
[20]	Xu X F,Yang X D,Zhang P,et al. Effects of exogenous galanin on neuropathic pain state and change of galanin and its receptors in DRG and SDH after sciatic nerve-pinch injury in rat[J]. Plos One,2012,7(5):e37621. doi: 10.1371/journal.pone.0037621
[21]	Dong Yan,Li Chongyang,Zhang Xiaomin,et al. The activation of galanin receptor 2 plays an antinociceptive effect in nucleus accumbens of rats with neuropathic pain[J]. J Physiol Sci,2021,71(1):6. doi: 10.1186/s12576-021-00790-5
[22]	Mengnan Li, Xiaomin Zhang, Chongyang Li, et al. Galanin receptor 2 is involved in galanin-induced analgesic effect by activating PKC and CaMKII in the nucleus accumbens of inflammatory pain rats [J]. Front Neurosci, 2021, 14（期号?）: 593331. Li Mengnan,Zhang Xiaomin,Li Chongyang,et al. Galanin receptor 2 is involved in galanin-induced analgesic effect by activating PKC and CaMKII in the nucleus accumbens of inflammatory pain rats[J]. Front Neurosci,2021,14:593331.

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