Effect of miR-29c-3p/IGF1 Molecular Axis on Activation，Proliferation and Apoptosis of Hepatic Stellate Cells

Liang ZHANG; Baoquan WANG; Xifeng LEI; Xu WANG; Yang KE; Wei ZHANG

doi:10.12259/j.issn.2095-610X.S20230926

Volume 44 Issue 9

Sep. 2023

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Liang ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Yang KE, Wei ZHANG. Effect of miR-29c-3p/IGF1 Molecular Axis on Activation，Proliferation and Apoptosis of Hepatic Stellate Cells[J]. Journal of Kunming Medical University, 2023, 44(9): 7-14. doi: 10.12259/j.issn.2095-610X.S20230926

Citation:

Liang ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Yang KE, Wei ZHANG. Effect of miR-29c-3p/IGF1 Molecular Axis on Activation，Proliferation and Apoptosis of Hepatic Stellate Cells[J]. Journal of Kunming Medical University, 2023, 44(9): 7-14. doi: 10.12259/j.issn.2095-610X.S20230926

Citation:

Liang ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Yang KE, Wei ZHANG. Effect of miR-29c-3p/IGF1 Molecular Axis on Activation，Proliferation and Apoptosis of Hepatic Stellate Cells[J]. Journal of Kunming Medical University, 2023, 44(9): 7-14. doi: 10.12259/j.issn.2095-610X.S20230926

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Effect of miR-29c-3p/IGF1 Molecular Axis on Activation，Proliferation and Apoptosis of Hepatic Stellate Cells

doi: 10.12259/j.issn.2095-610X.S20230926

Liang ZHANG¹,
Baoquan WANG¹,
Xifeng LEI¹,
Xu WANG¹,
Yang KE^{2
,
,},
Wei ZHANG^{1
,
,}

1.
Dept. of General Surgery，Weinan Central Hospital，Weinan Shaanxi 714000
2.
Dept. of Hepatobiliary and Pancreatic Surgery，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101，China

Received Date: 2023-04-26
Available Online: 2023-09-22
Publish Date: 2023-09-30

Abstract

Abstract

Objective To investigate the effects of miR-29c-3p on the activation, proliferation and apoptosis of hepatic stellate cells （HSCs） via IGF-1. Methods Primary mouse HSCs were cultured and the expression of HSCs marker ɑ-SMA was detected by immunofluorescence. By dual-luciferase reporter gene assay was conducted to Validate the Targeting Relationship between miR-29c-3p and IGF-1. After TGF-β activation of HSCs and exogenous regulation of miR-29c-3p and IGF-1 expression, the expression of activation-related proteins （ɑ-SMA, DDR2, FN1, ITGB1 and GFAP）, proliferation, colony-forming number and apoptosis in activated HSCs were detected by Western bolt, CCK-8, colony-forming unit assays and flow cytometry, respectively. Results Positive expression of ɑ-SMA indicated that the successful isolation of mouse HSCs. miR-29c-3p significantly reduced the luciferase activity of wild-type IGF-1, but had no effect on mutant IGF-1. Over-expression of miR-29c-3p and hypo-expression of IGF-1 significantly decreased ɑ-SMA, DDR2, FN1 and ITGB1 expression, increased GFAP expression, and decreased proliferation viability and colony-forming number of HSCs and upregulated their apoptotic ratio. Conclusion miR-29c-3p inhibits the activation and proliferation of HSCs and promotes their apoptosis by targeting IGF-1 expression.
- Alcoholic liver disease,
- Hepatic stellate cells,
- Activation,
- Proliferation,
- Apoptosis,
- miR-29c-3p,
- IGF-1

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References(30)

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