Molecular Epidemiological Investigation of HPV 56 in Precancerous Cervical Cancer Patients in Yunnan Province

Ziyun ZHOU; Yingying WANG; Zhiling YAN; Lingfeng ZHAO; Xi YANG; Jinmei XU; Shao ZHANG

doi:10.12259/j.issn.2095-610X.S20231005

Volume 44 Issue 10

Oct. 2023

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Ziyun ZHOU, Yingying WANG, Zhiling YAN, Lingfeng ZHAO, Xi YANG, Jinmei XU, Shao ZHANG. Molecular Epidemiological Investigation of HPV 56 in Precancerous Cervical Cancer Patients in Yunnan Province[J]. Journal of Kunming Medical University, 2023, 44(10): 114-121. doi: 10.12259/j.issn.2095-610X.S20231005

Citation:

Ziyun ZHOU, Yingying WANG, Zhiling YAN, Lingfeng ZHAO, Xi YANG, Jinmei XU, Shao ZHANG. Molecular Epidemiological Investigation of HPV 56 in Precancerous Cervical Cancer Patients in Yunnan Province[J]. Journal of Kunming Medical University, 2023, 44(10): 114-121. doi: 10.12259/j.issn.2095-610X.S20231005

Citation:

Ziyun ZHOU, Yingying WANG, Zhiling YAN, Lingfeng ZHAO, Xi YANG, Jinmei XU, Shao ZHANG. Molecular Epidemiological Investigation of HPV 56 in Precancerous Cervical Cancer Patients in Yunnan Province[J]. Journal of Kunming Medical University, 2023, 44(10): 114-121. doi: 10.12259/j.issn.2095-610X.S20231005

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Molecular Epidemiological Investigation of HPV 56 in Precancerous Cervical Cancer Patients in Yunnan Province

doi: 10.12259/j.issn.2095-610X.S20231005

1.
Center for Disease Control and Prevention，Qujing City，Qujing Yunnan 655000
2.
School of Basic Medicine，Kunming Medical University，Kunming Yunnan 650500
3.
Dept. of Gynaecologic Oncology，The 3rd Affiliated Hospital of Kunming Medical University， Kunming Yunnan 650118，China

Received Date: 2023-03-12
Available Online: 2023-09-11
Publish Date: 2023-10-25

Abstract

Abstract

Objective To study the genetic variation characteristics of HPV56 E6, E7 and L1 in patients with precancerous lesions infected with HPV56, and to obtain the molecular epidemiological data of HPV56 in patients with precancerous lesions of cervical cancer in Yunnan. Methods A total of 300 cervical intraepithelial neoplasia patients were enrolled and HPV genotyping kit was used to detect the types of HPV infection in the patients. The HPV E6, E7, and L1 genes in patients infected with HPV 56 were sequenced. Finally, the lineage distribution of HPV 56 and the genetic variations of HPV 56 E6, E7, and L1 genes in the study subjects were analyzed. Results In this study, the HPV56 virus infected by the study subjects mainly belonged to the A lineage, with more than 90% belonged to the A lineage, and about 10% belonged to the B lineage. There were 13 mutation loci in the E6, E7 and L1 genes of HPV56, including 6 in the E6 gene, 4 in the E7 gene and 3 in the L1 gene. Among the 6 mutation sites of HPV56 E6 gene, 2 were synonymous mutations （T158C （S19S）, G437A （P112P））, and 4 were non-synonymous mutations （A141C （S14R）, A241G （N47S）, A263C （K54N）, G279A （D60N））. The four mutation sites of HPV56 E7 gene were all non-synonymous mutations （C601A （D10E）, G605A （V12I）, G770C （E67Q）, G802C （Q77H））. Among the three mutation sites of HPV56 L1 gene, two were synonymous mutations （G6454T （P321P）, A6868C （I459I））, and one was non-synonymous mutation （A5867G （N126D））. Conclusion This study obtained preliminary data on the genetic variations of HPV 56 E6, E7, and L1 genes in CIN patients in Yunnan area, and obtained the molecular epidemiological characteristics of HPV 56 E6, E7, and L1 genes. It provides necessary data for further analysis of the molecular epidemiological characteristics of HPV 56 E6, E7, and L1 genes in CIN patients in Yunnan area.
- Cervical intraepithelial neoplasia,
- HPV56,
- Variation,
- Lineage

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References(28)

References

[1]	Arbyn M,Weiderpass E,Bruni L,et al. Estimates of incidence and mortality of cervical cancer in 2018: A worldwide analysis[J]. Lancet Glob Health,2020,8(2):e191-e203. doi: 10.1016/S2214-109X(19)30482-6
[2]	李双,李明珠,丛青,等. 人乳头瘤病毒疫苗临床应用中国专家共识[J]. 中国妇产科临床杂志,2021,22(2):225-234. doi: 10.13390/j.issn.1672-1861.2021.02.045
[3]	Burd E M. Human papillomavirus and cervical cancer[J]. Clin Microbiol Rev.,2003,16(1):1-17. doi: 10.1128/CMR.16.1.1-17.2003
[4]	Lorincz A T, Quinn A P, Goldsborough M D, et al. Human papillomavirus type 56: A new virus detected in cervical cancers [J]. J Gen Virol, 1989, 70 （ Pt 11）: 3099-3104.
[5]	Yang L,Yang H,Wu K,et al. Prevalence of HPV and variation of HPV 16/HPV 18 E6/E7 genes in cervical cancer in women in South West China[J]. J Med Virol,2014,86(11):1926-1936. doi: 10.1002/jmv.24043
[6]	Zhao S,Zhao X,Hu S,et al. Distribution of high-risk human papillomavirus genotype prevalence and attribution to cervical precancerous lesions in rural North China[J]. Chin J Cancer Res,2019,31(4):663-672. doi: 10.21147/j.issn.1000-9604.2019.04.10
[7]	Harari A,Chen Z,Burk R D. Human papillomavirus genomics: Past,present and future[J]. Curr Probl Dermatol,2014,45:1-18.
[8]	Zhou Z,Yang H,Yang L,et al. Human papillomavirus type 16 E6 and E7 gene variations associated with cervical cancer in a Han Chinese population[J]. Infect Genet Evol,2019,73:13-20. doi: 10.1016/j.meegid.2019.04.008
[9]	Wu Y, Chen Y, Li L, et al. Analysis of mutations in the E6/E7 oncogenes and L1 gene of human papillomavirus 16 cervical cancer isolates from China [J]. J Gen Virol, 2006, 87（Pt 5）: 1181-1188.
[10]	Lee C W,Bae J H,Lee S J,et al. Distribution of human papillomavirus type 16 E6 and E7 gene variants in the progression of cervical dysplasia in Korean women[J]. J Obstet Gynaecol Res,2011,37(10):1320-1326. doi: 10.1111/j.1447-0756.2010.01520.x
[11]	Sun Z,Ren G,Cui X,et al. Genetic diversity of HPV-16 E6,E7,and L1 genes in women with cervical lesions in Liaoning Province,China[J]. Int J Gynecol Cancer,2011,21(3):551-558. doi: 10.1097/IGC.0b013e3182112023
[12]	Buck C B,Day P M,Trus B L. The papillomavirus major capsid protein L1[J]. Virology,2013,445(1-2):169-174. doi: 10.1016/j.virol.2013.05.038
[13]	Jing Y,Wang T,Chen Z,et al. Phylogeny and polymorphism in the long control regions E6,E7,and L1 of HPV Type 56 in women from southwest China[J]. Mol Med Rep,2018,17(5):7131-7141.
[14]	Burk R D,Harari A,Chen Z. Human papillomavirus genome variants[J]. Virology,2013,445(1-2):232-243. doi: 10.1016/j.virol.2013.07.018
[15]	中华医学会. 临床诊疗指南. 妇产科学分册[M]. 北京: 人民卫生出版社, 2007: 69-73.
[16]	Munoz N,Bosch FX,de Sanjose S,et al. Epidemiologic classification of human papillomavirus types associated with cervical cancer[J]. N Engl J Med,2003,348(6):518-527. doi: 10.1056/NEJMoa021641
[17]	Franceschi S,Rajkumar R,Snijders P J,et al. Papillomavirus infection in rural women in southern India[J]. Br J Cancer,2005,92(3):601-606. doi: 10.1038/sj.bjc.6602348
[18]	Nielsen A,Kjaer S K,Munk C,et al. Type-specific HPV infection and multiple HPV types: Prevalence and risk factor profile in nearly 12,000 younger and older Danish women[J]. Sex Transm Dis,2008,35(3):276-282. doi: 10.1097/OLQ.0b013e31815ac5c7
[19]	Liu J,Ma S,Qin C,et al. Prevalence and genotype distribution of human papillomavirus in Zhengzhou,China,in 2016[J]. Arch Virol,2020,165(3):731-736. doi: 10.1007/s00705-019-04515-3
[20]	Wu H,Wu E,Ma L,et al. Lineage distribution and E2 sequence variation of high-risk human papillomavirus types isolated from patients with cervical cancer in Sichuan Province,China[J]. Arch Virol,2015,160(11):2845-2855. doi: 10.1007/s00705-015-2559-z
[21]	Dai S,Yao Y,Yan Z,et al. The association of human papillomavirus type 16 E2 variations with cervical cancer in a Han Chinese population[J]. Infect Genet Evol,2018,64:241-248. doi: 10.1016/j.meegid.2018.07.002
[22]	Morgan E L,Scarth J A,Patterson M R,et al. E6-mediated activation of JNK drives EGFR signalling to promote proliferation and viral oncoprotein expression in cervical cancer[J]. Cell Death Differ,2020,28(5):1669-1687.
[23]	Scarth J A,Patterson M R,Morgan E L,et al. The human papillomavirus oncoproteins: A review of the host pathways targeted on the road to transformation[J]. J Gen Virol,2021,102(3):001540.
[24]	Howie H L,Katzenellenbogen R A,Galloway D A. Papillomavirus E6 proteins[J]. Virology,2009,384(2):324-334. doi: 10.1016/j.virol.2008.11.017
[25]	Hiller T,Stubenrauch F,Iftner T. Isolation and functional analysis of five HPVE6 variants with respect to p53 degradation[J]. J Med Virol,2008,80(3):478-483. doi: 10.1002/jmv.21093
[26]	Roman A,Munger K. The papillomavirus E7 proteins[J]. Virology,2013,445(1-2):138-168. doi: 10.1016/j.virol.2013.04.013
[27]	Kirnbauer R,Taub J,Greenstone H,et al. Efficient self-assembly of human papillomavirus type 16 L1 and L1-L2 into virus-like particles[J]. J Virol,1993,67(12):6929-6936. doi: 10.1128/jvi.67.12.6929-6936.1993
[28]	Tan W,Felber B K,Zolotukhin A S,et al. Efficient expression of the human papillomavirus type 16 L1 protein in epithelial cells by using Rev and the Rev-responsive element of human immunodeficiency virus or the cis-acting transactivation element of simian retrovirus type 1[J]. J Virol,1995,69(9):5607-5620. doi: 10.1128/jvi.69.9.5607-5620.1995

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