Differences of Placental Microbiota DNA between Gestational Diabetes Mellitus and Normal Full-term Mothers

Qingyao YANG; Jie ZENG; Weiquan ZHANG; Yue NING; Meng LI; Zhanhua LI; Mei LIU; Jingjing XIONG; Yongkun HUANG

doi:10.12259/j.issn.2095-610X.S20231006

Volume 44 Issue 10

Oct. 2023

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2023 > 44(10): 140-148

Qingyao YANG, Jie ZENG, Weiquan ZHANG, Yue NING, Meng LI, Zhanhua LI, Mei LIU, Jingjing XIONG, Yongkun HUANG. Differences of Placental Microbiota DNA between Gestational Diabetes Mellitus and Normal Full-term Mothers[J]. Journal of Kunming Medical University, 2023, 44(10): 140-148. doi: 10.12259/j.issn.2095-610X.S20231006

Citation:

Qingyao YANG, Jie ZENG, Weiquan ZHANG, Yue NING, Meng LI, Zhanhua LI, Mei LIU, Jingjing XIONG, Yongkun HUANG. Differences of Placental Microbiota DNA between Gestational Diabetes Mellitus and Normal Full-term Mothers[J]. Journal of Kunming Medical University, 2023, 44(10): 140-148. doi: 10.12259/j.issn.2095-610X.S20231006

Citation:

Qingyao YANG, Jie ZENG, Weiquan ZHANG, Yue NING, Meng LI, Zhanhua LI, Mei LIU, Jingjing XIONG, Yongkun HUANG. Differences of Placental Microbiota DNA between Gestational Diabetes Mellitus and Normal Full-term Mothers[J]. Journal of Kunming Medical University, 2023, 44(10): 140-148. doi: 10.12259/j.issn.2095-610X.S20231006

PDF( 3561 KB)

Differences of Placental Microbiota DNA between Gestational Diabetes Mellitus and Normal Full-term Mothers

doi: 10.12259/j.issn.2095-610X.S20231006

Dept. of Pediatrics，The 1st Afiliated Hospital of Kunming Medical University， Kunming Yunnan 650032，China

Received Date: 2023-07-06
Available Online: 2023-09-12
Publish Date: 2023-10-25

Abstract

Abstract

Objective To explore the differences of maternal placental microbiota DNA between gestational diabetes mellitus （GDM） and normal full-term infants, and provide reference data for further research. Methods A total of 30 cases of full-term infants’ placental tissue were collected from February to September 2018 at the First Affiliated Hospital of Kunming Medical University. The cases were divided into two groups: the gestational diabetes group （Group A） and the normal full-term infants group （Group B）, based on the presence or absence of gestational diabetes. The bacterial DNA in the placental tissue was sequenced using the Illumina MiSeq sequencing system, and the differences in placental microbiota DNA between the two groups were compared. Results The DNA of microbial communities in the placental tissue was mainly composed of phyla such as Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes. LEfSe analysis showed no statistically significant differences in the microbial communities at the phylum and class levels between the two groups （P > 0.05）. However, at the genus level, the relative abundance of Propionibacterium, Thermus, Collinsella, Clostridium IV, Alistipes and Anaerostipes was significantly higher in the gestational diabetes group （LDA > 2）. Conclusion The results of our study indicates that there are a rich microbiome DNA in the maternal placenta and that the composition and abundance of microbiome DNA are different between GDM and normal full-term infants, which may affect the occurrence and prognosis of GDM and deserves further study.
- Placental microbiome,
- 16S rDNA high-throughput sequencing,
- Gestational diabetes mellitus,
- Birth of full-term infants

FullText(HTML)

References(31)

References

[1]	Giannella L,Grelloni C,Quintili D,et al. Microbiome changes in pregnancy disorders[J]. Antioxidants,2023,12(2):463. doi: 10.3390/antiox12020463
[2]	Dominguez-Bello M G,Godoy-Vitorino F,Knight R,et al. Role of the microbiome in human development[J]. Gut,2019,68(6):1108-1114. doi: 10.1136/gutjnl-2018-317503
[3]	Tozzo P,Delicati A,Caenazzo L. Human microbiome and microbiota identification for preventing and controlling healthcare-associated infections: A systematic review[J]. Front Public Health,2022,10:989496. doi: 10.3389/fpubh.2022.989496
[4]	Yao Y,Cai X,Chen C,et al. The role of microbiomes in pregnant women and offspring: Research progress of recent years[J]. Front Pharmacol,2020,11:643. doi: 10.3389/fphar.2020.00643
[5]	Farhat S,Hemmatabadi M,Ejtahed H S,et al. Microbiome alterations in women with gestational diabetes mellitus and their offspring: A systematic review[J]. Frontiers in Endocrinology,2022,13:1060488. doi: 10.3389/fendo.2022.1060488
[6]	Tang N,Luo Z C,Zhang L,et al. The association between gestational diabetes and microbiota in placenta and cord blood[J]. Frontiers in Endocrinology,2020,11:550319. doi: 10.3389/fendo.2020.550319
[7]	La X,Wang Y,Xiong X,et al. The composition of placental microbiota and its association with adverse pregnancy outcomes[J]. Front Microbiol,2022,13:911852. doi: 10.3389/fmicb.2022.911852
[8]	Zheng J,Xiao X,Zhang Q,et al. The placental microbiome varies in association with low birth weight in full-term neonates[J]. Nutrients,2015,7(8):6924-6937. doi: 10.3390/nu7085315
[9]	凌娅. 胎盘、阴道局部菌群改变与胎膜早破相关性研究[D]. 昆明: 昆明医科大学, 2022.
[10]	熊晶晶,祁文瑾,胡红卫,等. 正常足月儿母亲胎盘菌群检析[J]. 昆明医科大学学报,2017,38(11):52-57. doi: 10.3969/j.issn.1003-4706.2017.11.013
[11]	Wang H,Li N,Chivese T,et al. IDF diabetes atlas: Estimation of global and regional gestational diabetes mellitus prevalence for 2021 by international association of diabetes in pregnancy Study Group’s criteria[J]. Diabetes Research and Clinical Practice,2022,183:109050. doi: 10.1016/j.diabres.2021.109050
[12]	Li Y,Ren X,He L,et al. Maternal age and the risk of gestational diabetes mellitus: A systematic review and meta-analysis of over 120 million participants[J]. Diabetes Res Clin Pract,2020,162:108044. doi: 10.1016/j.diabres.2020.108044
[13]	Zhang Y,Xiao C M,Zhang Y,ea al. Factors associated with gestational diabetes mellitus: A meta-analysis[J]. J Diabetes Res,2021,2021:6692695.
[14]	Sun M,Luo M,Wang T,et al. Effect of the interaction between advanced maternal age and pre-pregnancy BMI on pre-eclampsia and GDM in Central China[J]. BMJ Open Diabetes Res Care,2023,11(2):e003324. doi: 10.1136/bmjdrc-2023-003324
[15]	Ehlers E,Talton O O,Schust D J,et al. Placental structural abnormalities in gestational diabetes and when they develop: A scoping review[J]. Placenta,2021,116:58-66. doi: 10.1016/j.placenta.2021.04.005
[16]	Xie W,Wang Y,Xiao S,et al. Association of gestational diabetes mellitus with overall and type specific cardiovascular and cerebrovascular diseases: Systematic review and meta-analysis[J]. BMJ,2022,378:e070244.
[17]	Johns E C,Denison F C,Norman J E,et al. Gestational diabetes mellitus: Mechanisms,treatment,and complications[J]. Trends Endocrinol Metab,2018,29(11):743-754. doi: 10.1016/j.tem.2018.09.004
[18]	Ye W,Luo C,Huang J,Li C,et al. Gestational diabetes mellitus and adverse pregnancy outcomes: Systematic review and meta-analysis[J]. BMJ,2022,377:e067946.
[19]	Wu S,Jin J,Hu K L,et al. Prevention of gestational diabetes mellitus and gestational weight gain restriction in overweight/obese pregnant women: A systematic review and network meta-analysis[J]. Nutrients,2022,14(12):2383. doi: 10.3390/nu14122383
[20]	Murray S R,Reynolds R M. Short- and long-term outcomes of gestational diabetes and its treatment on fetal development[J]. Prenat Diagn,2020,40(9):1085-1091. doi: 10.1002/pd.5768
[21]	Tarry-Adkins J L,Aiken C E,Ozanne S E. Neonatal,infant,and childhood growth following metformin versus insulin treatment for gestational diabetes: A systematic review and meta-analysis[J]. PLoS Med,2019,16(8):e1002848. doi: 10.1371/journal.pmed.1002848
[22]	Homayouni A,Bagheri N,Mohammad-Alizadeh-Charandabi S,et al. Prevention of gestational diabetes mellitus (GDM) and probiotics: Mechanism of action: A review[J]. Curr Diabetes Rev,2020,16(6):538-545.
[23]	Deng Y F,Wu L P,Liu Y P. Probiotics for preventing gestational diabetes in overweight or obese pregnant women: A review[J]. World J Clin Cases,2022,10(36):13189-13199. doi: 10.12998/wjcc.v10.i36.13189
[24]	Zhou L,Ding C,Wu J,et al. Probiotics and synbiotics show clinical efficacy in treating gestational diabetes mellitus: A meta-analysis[J]. Prim Care Diabetes,2021,15(6):937-947. doi: 10.1016/j.pcd.2021.08.005
[25]	Okesene-Gafa K A,Moore A E,Jordan V,et al. Probiotic treatment for women with gestational diabetes to improve maternal and infant health and well-being[J]. Cochrane Database Syst Rev,2020,6(6):CD012970.
[26]	Dolatkhah N,Hajifaraji M,Abbasalizadeh F,et al. Is there a value for probiotic supplements in gestational diabetes mellitus? A randomized clinical trial[J]. J Health Popul Nutr,2015,33:25. doi: 10.1186/s41043-015-0034-9
[27]	Zakaria Z Z,Al-Rumaihi S,Al-Absi R S,et al. Physiological changes and interactions between microbiome and the host during pregnancy[J]. Front Cell Infect Microbiol,2022,12:824925. doi: 10.3389/fcimb.2022.824925
[28]	Davidson S J,Barrett H L,Price S A,et al. Probiotics for preventing gestational diabetes[J]. Cochrane Database Syst Rev,2021,4(4):CD009951.
[29]	Zheng J,Xiao X,Zhang Q,et al. The placental microbiota is altered among subjects with gestational diabetes mellitus: A pilot study[J]. Front Physiol,2017,8:675. doi: 10.3389/fphys.2017.00675
[30]	Bassols J,Serino M,Carreras-Badosa G,et al. Gestational diabetes is associated with changes in placental microbiota and microbiome[J]. Pediatr Res,2016,80(6):777-784. doi: 10.1038/pr.2016.155
[31]	Zakis DR,Paulissen E,Kornete L,et al. The evidence for placental microbiome and its composition in healthy pregnancies: A systematic review[J]. J Reprod Immunol,2022,149:103455. doi: 10.1016/j.jri.2021.103455

Relative Articles(20)

Relative Articles

[1]	Nan LI, Ziyi NIU, Lan SHAO. Gastrodin Ameliorates Maternal-Fetal Interface Injury in Gestational Diabetes Mellitus Rats by Activating the GPR120/AMPK Pathway. Journal of Kunming Medical University, 2026, 47(2): 1-8.
[2]	Yan CHI, Junxian LI, Ling ZHAO, Wenyi LI, Tingyu KE. Correlation Analysis between Coagulation and Fibrinolysis in Early Pregnancy and Gestational Diabetes in Women with Different BMI before Pregnancy. Journal of Kunming Medical University, 2025, 46(4): 77-82. doi: 10.12259/j.issn.2095-610X.S20250410
[3]	Yuzhen ZHAO, Yingyan MA, Lili HE. Analysis of the Value of Serum Testosterone，Sex Hormone-Binding Globulin and Vitamin D Levels in Predicting Gestational Diabetes Mellitus Complicated by Polycystic Ovary Syndrome. Journal of Kunming Medical University, 2025, 46(8): 1-7.
[4]	Yaling ZHAO, Kun WU, Kai WANG, Rong HUANG, Genya HE, Yuhui NA, Miao HE, Zhenbo DING, Caiying ZHANG. Influence of Intrauterine Hyperglycemia Environment on Peripheral Inflammatory Response in Offspring of Gestational Diabetes Mellitus. Journal of Kunming Medical University, 2023, 44(11): 70-75. doi: 10.12259/j.issn.2095-610X.S20231110
[5]	Yindong HE, Jun ZHANG, Hua HUANG, Ruming LIU, Xi WANG. Glucose Transport in Human Placenta and the Effect of GDM on Placental Glucose Transport. Journal of Kunming Medical University, 2022, 43(9): 160-165. doi: 10.12259/j.issn.2095-610X.S20220903
[6]	Shan-shan GUO, Ji-hong LIU. Correlation between Changes of Vaginal Microflora and Premature Rupture of Membranes during Pregnancy. Journal of Kunming Medical University, 2021, 42(11): 38-44. doi: 10.12259/j.issn.2095-610X.S20211107
[7]	Yin Shun Hui , Zhan Ye Ming , Jia Feng Mei , Ran Li Quan , Zhang Ming Zhu . . Journal of Kunming Medical University, 2019, 40(04): 23-30.
[8]	Zhang Lin Jing , Huang Qin , Wei Dao . . Journal of Kunming Medical University, 2019, 40(11): 97-100.
[9]	Yang Yi Mei , Kang Wei , Fan Wei , Dai Fu Rong , Sun Yu . . Journal of Kunming Medical University, 2019, 40(09): 96-100.
[10]	Su Hong , Sun Hong Li , Yang Jun . . Journal of Kunming Medical University, 2018, 39(09): 33-36.
[11]	Li Xiao Hong , Ma Run Mei , Chen Zhuo . . Journal of Kunming Medical University, 2017, 38(05): 64-67.
[12]	Xiong Jing Jing , Qi Wen Jin , Hu Hong Wei , Zeng Jie , Huang Rong , Ma Run Mei , Liu Mei , Huang Yong Kun . The Detection and Analysis of the Placenta Microbiota of Normal Term Delivery. Journal of Kunming Medical University, 2017, 38(11): 52-57.
[13]	Zhang Xin Wen , Zhang Yan , Hu Yan Xin , Pan Bao Long , Wang Ming Fang , Ma Run Mei . Establishment of Gestational Diabetes Rat Model Induced by High-fat Diet. Journal of Kunming Medical University, 2016, 37(11): 8-13.
[14]	Zhang Mi Li . . Journal of Kunming Medical University,
[15]	Li Hai Xia . . Journal of Kunming Medical University,
[16]	Chen Wei Wen . Clinical Study of Subclinical Hypothyroidism during Pregnancy. Journal of Kunming Medical University,
[17]	Wei Meng Nan . . Journal of Kunming Medical University,
[18]	Zhang Yi . . Journal of Kunming Medical University,
[19]	. Insulin Resistance and β-cell Secretion in Women with Normal Glucose Tolerance and Gestational Diabetes Mellitus. Journal of Kunming Medical University,
[20]	. Comparison of the Clinical Characteristics of Newborn Respiratory Distress Syndrome between Full-term and Premature Infants. Journal of Kunming Medical University,