Analgesic Effect of Needle-knife Therapy on Rats with Neuropathic Pain

Yanan LIU; Shuang YANG; Yuhang PENG; Jing CHEN; Shilian XU

doi:10.12259/j.issn.2095-610X.S20231009

Volume 44 Issue 10

Oct. 2023

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Yanan LIU, Shuang YANG, Yuhang PENG, Jing CHEN, Shilian XU. Analgesic Effect of Needle-knife Therapy on Rats with Neuropathic Pain[J]. Journal of Kunming Medical University, 2023, 44(10): 33-38. doi: 10.12259/j.issn.2095-610X.S20231009

Citation:

Yanan LIU, Shuang YANG, Yuhang PENG, Jing CHEN, Shilian XU. Analgesic Effect of Needle-knife Therapy on Rats with Neuropathic Pain[J]. Journal of Kunming Medical University, 2023, 44(10): 33-38. doi: 10.12259/j.issn.2095-610X.S20231009

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Analgesic Effect of Needle-knife Therapy on Rats with Neuropathic Pain

doi: 10.12259/j.issn.2095-610X.S20231009

Yanan LIU^{1, 2},
Shuang YANG¹,
Yuhang PENG¹,
Jing CHEN^{1
,
,},
Shilian XU^{1
,
,}

1.
School of Basic Medicine，Kunming Medical University，Kunming Yunnan 650500
2.
Secondary Vocational College，Shandong Chemical-Engineering Vocational College， Weifang Shandong 261100，China

Received Date: 2023-06-28
Available Online: 2023-09-08
Publish Date: 2023-10-25

Abstract

Abstract

Objective To explore the antinociception of acupotomology in rats with sciatic nerve ligation, so as to provide experimental basis for the clinical application and promotion of needle-knife therapy on neuralgia. Methods The hot-plate test was used to measure the hindpaw withdrawal latency （HWL） induced by thermal stimuli, and the Randall-Selitto test was used to measure the hindpaw withdrawal threshold （HWT） induced by pressure stimuli as indicators of pain threshold. The chronic constriction injury （CCI） model was established by partial ligation of the left sciatic nerve to create neuropathic pain in rats. The CCI rats were randomly divided into a model group, a control group, and a needle-knife treatment group, and the pain thresholds of each group of rats were measured. The CCI rats were further randomly divided into a morphine group （subcutaneous injection of morphine 10 mg/kg） and a needle-knife treatment group （needle-knife intervention）, and the pain thresholds in each group of rats were observed. Results Compared with before ligation, bilateral HWL and HWT of rats decreased after left sciatic nerve ligation at 10 d （HWL: P_left < 0.01, P_right < 0.01; HWT: P_left < 0.001, P_right < 0.001）, 20 d （HWL: P_left < 0.001, P_right < 0.001; HWT: P_left < 0.001, P_right < 0.001） and 30 d （HWL: P_left < 0.001, P_right < 0.05; HWT: P_left < 0.001, P_right < 0.001）. Compared with the control group, the HWL （left: P < 0.001; right: P < 0.001） and HWT （left: P < 0.001; right: P < 0.001） of rats in the needle-knife group were significantly prolonged; compared with the model group, the HWL （left: P < 0.001; right: P < 0.001） and HWT （left: P < 0.001; right: P < 0.001） of rats in the needle-knife group were also significantly prolonged. However, there was no significant difference between the model group and the control group （P > 0.05）. Compared with the morphine group, the analgesic effect of the needle-knife group on neuropathic pain was stable and lasted longer, while the subcutaneous injection of morphine had a significant analgesic effect at 20-180 min after injection, but its analgesic effect weakened or even disappeared with the prolongation of time. Conclusion Needle-knife therapy has an analgesic effect on CCI rats. Compared with morphine, the analgesic effect of needle- knife therapy is more stable and lasting.
- Needle- knife,
- Neuralgia,
- Analgesic effect

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References(17)

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