Spatiotemporal Expression Profiles of miR-25 in <i>Siniperca chuatsi </i>and Prediction Analysis of Its Targeting Core Circadian Clock Genes

Yi ZHOU; Kai MI; Xiaoli FENG

doi:10.12259/j.issn.2095-610X.S20231010

Volume 44 Issue 10

Oct. 2023

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2023 > 44(10): 55-59

Yi ZHOU, Kai MI, Xiaoli FENG. Spatiotemporal Expression Profiles of miR-25 in Siniperca chuatsi and Prediction Analysis of Its Targeting Core Circadian Clock Genes[J]. Journal of Kunming Medical University, 2023, 44(10): 55-59. doi: 10.12259/j.issn.2095-610X.S20231010

Citation:

Yi ZHOU, Kai MI, Xiaoli FENG. Spatiotemporal Expression Profiles of miR-25 in Siniperca chuatsi and Prediction Analysis of Its Targeting Core Circadian Clock Genes[J]. Journal of Kunming Medical University, 2023, 44(10): 55-59. doi: 10.12259/j.issn.2095-610X.S20231010

Citation:

PDF( 991 KB)

Spatiotemporal Expression Profiles of miR-25 in Siniperca chuatsi and Prediction Analysis of Its Targeting Core Circadian Clock Genes

doi: 10.12259/j.issn.2095-610X.S20231010

College of Basic Medicine，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2023-06-27
Available Online: 2023-09-11
Publish Date: 2023-10-25

Abstract

Abstract

Objective To study the spatiotemporal expression profiles of miR-25 in the Chinese perch（Siniperca chuatsi）, to predict its targeting core Circadian clock genes, and to explore its possible biological significance. Methods Real-time fluorescence quantitative PCR was used to analyze the expression characteristics of miR-25 in various tissues and embryos at different stages of Siniperca chuatsi, and TargetScan was used to predict its targeted core clock genes. Results The expression level of miR-25 was higher in the red muscle and heart （P < 0.05）, while it was lower in the spleen, kidney, intestine, and liver of the Chinese perch. However, the expression level difference in these four tissues was not statistically significant （P > 0.05）. The expression level of miR-25 was higher in the phase 2 of cell cycle in embryos （P < 0.05）, and it gradually decreased as the embryos developed. Target gene prediction results showed that the 3’UTR region of the rhythmic genes Arntl2 and Nr1d2b mRNA contained target sites for miR-25. Conclusion miR-25 exhibited maternal expression during embryonic development and may have important regulatory significance in the red muscle and heart of Chinese perch. Additionally, miR-25 may regulate the circadian rhythm by targeting the expression of Arntl2 and Nr1d2b, providing a reference for further research on the physiological effects of miR-25 and the regulation of circadian rhythm to prevent related diseases.
- Siniperca chuatsi,
- miR-25,
- Expression pattern,
- Target gene prediction

FullText(HTML)

References(22)

References

[1]	Makeyev E V,Maniatis T. Multilevel regulation of gene expression by microRNAs[J]. Science,2008,319(5871):1789-1790. doi: 10.1126/science.1152326
[2]	Schmiedel J M,Klemm S L,Zheng Y N,et al. Gene expression. MicroRNA control of protein expression noise[J]. Science,2015,348(6230):128-132. doi: 10.1126/science.aaa1738
[3]	丁小丽,胡蓉. microRNA-25在肿瘤中的研究进展[J]. 重庆医学,2017,46(4):545-548. doi: 10.3969/j.issn.1671-8348.2017.04.042
[4]	Sárközy M,Kahán Z,Csont T. A myriad of roles of miR-25 in health and disease[J]. Oncotarget,2018,9(30):21580-21612. doi: 10.18632/oncotarget.24662
[5]	Yang L Q,Li L N,Chang P,et al. MiR-25 regulates gastric cancer cell growth and apoptosis by targeting EGR2[J]. Frontiers in Genetics,2021,12:690196. doi: 10.3389/fgene.2021.690196
[6]	李卿,李轶君,张国锐. miR-25对食管癌EC109细胞侵袭和迁移能力的影响及临床意义[J]. 天津医药,2022,50(4):357-362.
[7]	董超,黄远丽,徐正丰. miR-25在乳腺癌组织、细胞的表达及对细胞增殖的影响[J]. 山东医药,2018,58(9):31-33. doi: 10.3969/j.issn.1002-266X.2018.09.009
[8]	王博,田园园,孙成飞,等. 翘嘴鳜microRNA转录组分析及生长相关miRNA鉴定[J]. 基因组学与应用生物学,2017,36(2):603-613. doi: 10.13417/j.gab.036.000603
[9]	Huang W,Ramsey K M,Marcheva B,et al. Circadian rhythms,sleep,and metabolism[J]. Clin Invest,2011,121(6):2133-2141. doi: 10.1172/JCI46043
[10]	Ketelauri P,Scharov K,von Gall C,et al. Acute circadian disruption due to constant light promotes Caspase 1 activation in the mouse hippocampus[J]. Cells,2023,12(14):1836. doi: 10.3390/cells12141836
[11]	Park I,Kim D,Kim J,et al. MicroRNA-25 as a novel modulator of circadian Period2 gene oscillation[J]. Experimental & Molecular Medicine,2020,52(9):1614-1626.
[12]	朱鑫,叶苏杭,李源,等. 鳜miR-21的时空表达特征及短期饥饿胁迫下的适应性节律表达调控[J]. 中国水产科学,2022,29(5):665-672. doi: 10.12264/JFSC2021-0520
[13]	朱鑫,秦家鑫,何雅芝,等. 翘嘴鳜miR-125b的时空表达特征及饥饿对其节律性表达的调控[J]. 基因组学与应用生物学,2023,42(1):34-43. doi: 10.13417/j.gab.042.000034
[14]	Kelu J J,Pipalia T G,Hughes S M. Circadian regulation of muscle growth independent of locomotor activity[J]. Proc Natl Acad Sci U S A,2020,117(49):31208-31218. doi: 10.1073/pnas.2012450117
[15]	刘希良,宾石玉,王开卓,等. 翘嘴鳜的人工繁殖与胚胎发育观察[J]. 广西师范大学学报(自然科学版),2013,31(2):100-106.
[16]	Chu W Y,Liu L S,Li Y L,et al. Systematic identification and differential expression profiling of MicroRNAs from white and red muscles of siniperca chuatsi[J]. Curr Mol Med,2013,13(8):1397-1407. doi: 10.2174/15665240113139990059
[17]	Livak K J,Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method[J]. Methods,2001,25(4):402-408. doi: 10.1006/meth.2001.1262
[18]	Wang B,Xu M T,Li M M,et al. MiR-25 promotes cardiomyocyte proliferation by targeting FBXW7[J]. Mol Ther Nucleic Acids,2020,19:1299-1308. doi: 10.1016/j.omtn.2020.01.013
[19]	Li H Z,Xie Y M,Liu Y S,et al. Alteration in microRNA-25 expression regulate cardiac function via renin secretion[J]. Exp Cell Res,2018,365(1):119-128. doi: 10.1016/j.yexcr.2018.02.029
[20]	任秀美奥,姚旭东,蒙亚琦,等. miR-449b对绵羊早期胚胎发育的影响[J]. 中国畜牧兽医,2022,49(7):2661-2668. doi: 10.16431/j.cnki.1671-7236.2022.07.024
[21]	Giraldez A J,Mishima Y,Rihel J,et al. Zebrafish miR-430 promotes deadenylation and clearance of maternal mRNAs[J]. Science,2006,312(5770):75-79. doi: 10.1126/science.1122689
[22]	Li X,Zhang W B,Fu J,et al. MicroRNA-451 is downregulated in the follicular fluid of women with endometriosis and influences mouse and human embryonic potential[J]. Reprod Biol Endocrinol,2019,17(1):96. doi: 10.1186/s12958-019-0538-z

Relative Articles(20)

Relative Articles

[1]	Chunyan LIU, Bingqing CHANG, Chao LI, Xin REN, Xiaoqin LIU. The Relationship between T Lymphocyte Subsets and Pathological Characteristics of Acute Myeloid Leukemia and the Value of Predicting Chemotherapy Prognosis. Journal of Kunming Medical University, 2024, 45(5): 116-122. doi: 10.12259/j.issn.2095-610X.S20240518
[2]	Peilin CUI, Yuxia SONG, Xuejuan WANG. Predictive Value of Serum and Tissue miR-205 for Postoperative Recurrence of Ovarian-type Endometriosis. Journal of Kunming Medical University, 2024, 45(10): 105-110. doi: 10.12259/j.issn.2095-610X.S20241016
[3]	Zhibo MIN, Ran ZHOU, Dan PU, Tao YANG. Expression of ANXA8，PGRMC1，and miR-92a in Cervical Cancer and Their Relationship with Clinicopathological Features. Journal of Kunming Medical University, 2024, 45(8): 101-108. doi: 10.12259/j.issn.2095-610X.S20240815
[4]	Runlin FENG, Zongqi DENG, Mengyao WU, Yunna WANG, Yu WANG, Guilan LIU. GJB4 Gene Expression in Relation to Clinical and Pathological Features of Pancreatic Cancer Patients. Journal of Kunming Medical University, 2024, 45(8): 1-9.
[5]	Chunyan LIU, Bingqing CHANG, Chao LI, Xin REN, Xiaoqin LIU. Correlations between CD4⁺CD25⁺Treg，sCD30 and Clinical Characteristics of Lymphoma and Their Value in Predicting Infection after Chemotherapy. Journal of Kunming Medical University, 2024, 45(4): 1-6.
[6]	Gaobo DU, Xiaofeng JIA, Qiang YU, Xiaojuan LI. Correlation of the Expression of miR-192 in Serum Expression with Left Ventricular Hypertrophy in Patients with Essential Hypertension. Journal of Kunming Medical University, 2023, 44(2): 139-143. doi: 10.12259/j.issn.2095-610X.S20230204
[7]	Shusheng LIAO, Hong CHEN, Mei LIU, Chengyu PAN, Aidi LUO, Li ZHANG. Clinical Characteristics and Genetic Testing of A Pedigree with Early-onset Parkinson's Disease Caused by PRKN Gene Mutation. Journal of Kunming Medical University, 2023, 44(5): 66-71. doi: 10.12259/j.issn.2095-610X.S20230504
[8]	Wei YANG, Hongyan CHEN, Wendong CHEN, Yanqiong WANG, Xiangfeng BAI. Effects of miR-208a on Myocardial Ischemia-reperfusion Injury by Regulating the Expression of QKI5 in Rats. Journal of Kunming Medical University, 2022, 43(7): 18-24. doi: 10.12259/j.issn.2095-610X.S20220724
[9]	Lianyong CHEN, Haohao RU, Xing YANG, Shuangqun YAN, Tao CHEN, Qinxuan NI, Lin XU. Characteristics of katG and inhA Gene Mutations in INH-Resistant Mycobacterium Tuberculosis in Yunnan Province. Journal of Kunming Medical University, 2022, 43(8): 28-33. doi: 10.12259/j.issn.2095-610X.S20220803
[10]	Jing-mei LUO, Can-hui LI, Lan LI, Jie LIN, Jin-rong ZHANG, Li-hua ZHANG. Expression and Significance of Plasma miR-1181 in Patients with Type 2 Diabetes Mellitus. Journal of Kunming Medical University, 2021, 42(10): 61-67. doi: 10.12259/j.issn.2095-610X.S20211019
[11]	Hong-fei ZHANG, De-long GE, Xiao-fei HUANG, Di MEI, Li GUO, Yi-rong WANG, Hai-mei LI, Tong-yi SUN, Guang-tao JIA, Wen-hui LI. Traditional Chinese Medicine Transcriptome Analysis of Characteristic Distribution of Scorpion Genes in Venomous Glands. Journal of Kunming Medical University, 2021, 42(5): 35-40. doi: 10.12259/j.issn.2095-610X.S20210507
[12]	Yang LIU, Jing LIAO, You-hui LU, Chun-yi SUN, Yu-shi MENG. Negative Regulation of hTERT Expression in Cervical Cancer Hela Cell Line by miR-21. Journal of Kunming Medical University, 2021, 42(3): 23-28. doi: 10.12259/j.issn.2095-610X.S20210307
[13]	Dai Hai Long , Zhang Xing , Pan Yun , Feng Xiao Lan , Lu Xiang Ting , Guang Xue Feng , Yin Xiao Long . . Journal of Kunming Medical University, 2020, 41(04): 60-63.
[14]	Ye Qiu Wen , Zou Tian Ning , Li Cheng Cheng , Song Ting Ting , Tan Jing . . Journal of Kunming Medical University, 2018, 39(12): 28-32.
[15]	Lei Xi Feng , Hou Feng Qiang , Yang Shao Hua , Zhang Wei . . Journal of Kunming Medical University, 2018, 39(12): 49-53.
[16]	Wu Gang . HBx Suppresses miR-338 Expression in Hepatoma via DNA Hypermethylation. Journal of Kunming Medical University, 2017, 38(11): 5-8.
[17]	Zhao Bin , Li Rui Qian , Zhang Guo Ying , Lei Yong Hong , Wang Qi Lin , Liu Zhi Min . Overexpression of miR-4328 Inhibits the Proliferation, Migration and Epithelial Mesenchymal Transition of Prostate Cancer Cells. Journal of Kunming Medical University, 2017, 38(11): 29-33.
[18]	Chen Bo . The Expression of Dicer， miR-10b and Its Precursor in Breast Cancer. Journal of Kunming Medical University,
[19]	Cao Yong Mei , Lv Yi , Liu Yu Jing , Li Ying Chuan . The Effect of miR-200 and Their Targets ZEB1/2 on Epithelial-mesenchymal Transition(EMT) in Early Pulmonary Fibrosis Caused by Acute Lung Injury. Journal of Kunming Medical University, 2016, 37(12): 1-7.
[20]	Chen Bo . . Journal of Kunming Medical University,