Protective Effect of Corilagin on OX-LDL-Induced HUVECs Cell Damage and Its Impact on the Expression of the MyD88 Signaling Pathway

Daiju TAO; Peng CHEN; Yuqing LI; Linyi CHEN; Renhua YANG; Bo HE; Zhiqiang SHEN

doi:10.12259/j.issn.2095-610X.S20231019

Volume 44 Issue 10

Oct. 2023

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Zhang Jian Hua . Clinical Application of MDT Strategy in the Diagnosis and Treatment of Bone and Soft Tissue Tumors[J]. Journal of Kunming Medical University, 2016, 37(09).

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Daiju TAO, Peng CHEN, Yuqing LI, Linyi CHEN, Renhua YANG, Bo HE, Zhiqiang SHEN. Protective Effect of Corilagin on OX-LDL-Induced HUVECs Cell Damage and Its Impact on the Expression of the MyD88 Signaling Pathway[J]. Journal of Kunming Medical University, 2023, 44(10): 18-25. doi: 10.12259/j.issn.2095-610X.S20231019

Zhang Jian Hua . Clinical Application of MDT Strategy in the Diagnosis and Treatment of Bone and Soft Tissue Tumors[J]. Journal of Kunming Medical University, 2016, 37(09).

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Protective Effect of Corilagin on OX-LDL-Induced HUVECs Cell Damage and Its Impact on the Expression of the MyD88 Signaling Pathway

doi: 10.12259/j.issn.2095-610X.S20231019

School of Pharmaceutical Science/Yunnan Key Laboratory of Pharmacology for Natural Products，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2023-05-31
Available Online: 2023-09-07
Publish Date: 2023-10-25

Abstract

Abstract

Objective To investigate the protective effects of different concentrations and durations of corilagin treatment on oxidative low-density lipoprotein （ox-LDL）-induced damage in human umbilical vein endothelial cells （HUVECs）, as well as its effects on the expression regulation of the MyD88 signaling pathway. Methods In vitro cultured HUVECs were used to replicate the ox-LDL-induced damage model. Morphological and immunological methods were employed to identify HUVECs cells. The MTT assay was used to determine the optimal conditions for replicating the ox-LDL-induced damage model in HUVECs. Cells were divided into different groups based on different treatments: normal group, model group, Corilagin （3.125, 6.25, 12.5, 25, 50 µmol/L） group, and positive control （VE 10 µmol/L, Simvastatin 1 µmol/L） group. The protective effect of corilagin on ox-LDL-induced damage in HUVECs was observed. Western blot and RT-qPCR methods were used to detect the expression changes of MyD88, P65, TNF-α, and MCP-1 in HUVECs cells. Results The cultured cells were confirmed as HUVECs by using morphological and immunological methods. The best condition for replicating ox-LDL-induced damage to HUVECs was 12 hours of treatment with 70 mg/L of ox-LDL. MTT results showed that compared with the ox-LDL group, the corilagin group had a significantly higher cell viability （P < 0.01）. RT-qPCR and Western blot results showed that compared with the control group, the mRNA and protein expression of MyD88, P65, TNF-α, and MCP-1 were increased in the ox-LDL group （P < 0.01）. Compared with the ox-LDL group, the mRNA and protein expression of MyD88, P65, TNF-α, and MCP-1 were decreased （P < 0.01） in the corilagin group and positive control group, and the corilagin group showed a dose-dependent downregulation of the mRNA and protein expression of MyD88, P65, TNF-α, and MCP-1. Conclusion With the increase of time and concentration, corilagin can significantly improve the cell viability of HUVECs induced by ox-LDL damage, and its protective effect is associated with the inhibition of the MyD88 signaling pathway.
- Corilagin,
- HUVECs,
- Ox-LDL,
- Atherosclerosis

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