Effect of Pristimerin on Proliferation of Oral Squamous Cell Carcinoma CAL-27 by Regulating Autophagy

Shiyuan YANG; Hao ZHANG; Tuqiang HU

doi:10.12259/j.issn.2095-610X.S20231024

Volume 44 Issue 10

Oct. 2023

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Shiyuan YANG, Hao ZHANG, Tuqiang HU. Effect of Pristimerin on Proliferation of Oral Squamous Cell Carcinoma CAL-27 by Regulating Autophagy[J]. Journal of Kunming Medical University, 2023, 44(10): 92-99. doi: 10.12259/j.issn.2095-610X.S20231024

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Shiyuan YANG, Hao ZHANG, Tuqiang HU. Effect of Pristimerin on Proliferation of Oral Squamous Cell Carcinoma CAL-27 by Regulating Autophagy[J]. Journal of Kunming Medical University, 2023, 44(10): 92-99. doi: 10.12259/j.issn.2095-610X.S20231024

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Effect of Pristimerin on Proliferation of Oral Squamous Cell Carcinoma CAL-27 by Regulating Autophagy

doi: 10.12259/j.issn.2095-610X.S20231024

Shiyuan YANG¹,
Hao ZHANG^{1, 2},
Tuqiang HU^{2
,
,}

1.
School of Stomatology，Hubei University of Medicine，Shiyan Hubei 442000
2.
Institute of Stomatology，Shiyan Renmin Hospital/ Affiliated Hospital of Hubei University of Medicine，Shiyan Hubei 442000，China

Received Date: 2023-07-05
Available Online: 2023-09-05
Publish Date: 2023-10-25

Abstract

Abstract

Objective To investigate the effect of pristimerin on the proliferation ability of human oral squamous cell carcinoma cells CAL-27 and its related mechanisms. Methods CCK-8 assay was performed to determine the proliferative bioactivity of CAL-27 cells treated with different concentrations of pristimerin, and the IC50 was calculated for different time periods. Colony formation assay was used to detect the cloning ability of CAL-27 cells. Western blotting was used to detect the protein expression levels of the proliferation marker PCNA and the autophagy flux markers BECLIN1, LC3B-II, and LC3B-I. CAL-27 cells were treated with pristimerin in combination with the autophagy inducer rapamycin （RAPA）, and Western blotting was used to detect the protein expression levels of PCNA and the autophagy flux markers BECLIN1, LC3B-II, and LC3B-I. Results Compared with the blank group, the pristimerin at various concentration gradients can inhibit the viability of CAL-27 cells （P < 0.05）, and it exhibits concentration-time dependence. Compared with the blank group, the expression levels of PCNA, BECLIN1 and LC3B-II/LC3B-I proteins in cells were significantly upregulated after treatment with the pristimerin （P < 0.05）. Compared with the group treated with only the pristimerin, the combined treatment of the pristimerin with the autophagy activator rapamycin （RAPA） enhanced the cell activity of CAL-27 cells （P < 0.05）, and the expression levels of PCNA, BECLIN1, and LC3B-II/LC3B-I proteins were significantly upregulated （P < 0.05）. Conclusion Pristimerin significantly inhibits the proliferation of CAL-27 oral squamous carcinoma cells in vitro, which may be associated with downregulation of autophagy-related genes BECLIN1, LC3B-II, and LC3B-I.
- Pristimerin,
- Oral squamous cell carcinoma,
- Proliferation,
- Autophagy

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References(26)

References

[1]	Liu J,Jiang X,Zou A,et al. CircIGHG-induced epithelial-to-mesenchymal transition promotes oral squamous cell carcinoma progression via miR-142-5p/igf2bp3 signaling[J]. Cancer Res,2021,81(2):344-355. doi: 10.1158/0008-5472.CAN-20-0554
[2]	Sung H,Ferlay J,Siegel R L,et al. Global cancer statistics 2020: Globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin,2021,71(3):209-249. doi: 10.3322/caac.21660
[3]	Yang Z,Yan G,Zheng L,et al. YKT6,as a potential predictor of prognosis and immunotherapy response for oral squamous cell carcinoma,is related to cell invasion,metastasis,and CD8+ T cell infiltration[J]. Oncoimmunology,2021,10(1):1938890. doi: 10.1080/2162402X.2021.1938890
[4]	Ling Z,Cheng B,Tao X. Epithelial-to-mesenchymal transition in oral squamous cell carcinoma: Challenges and opportunities[J]. Int J Cancer,2021,148(7):1548-1561. doi: 10.1002/ijc.33352
[5]	Khadela A,Shah Y,Mistry P,et al. Immunomodulatory therapy in head and neck squamous cell carcinoma: Recent advances and clinical prospects[J]. Technol Cancer Res Treat,2023,22:2081074849.
[6]	Debnath J,Gammoh N,Ryan K M. Autophagy and autophagy-related pathways in cancer[J]. Nat Rev Mol Cell Biol,2023,24(8):560-575. doi: 10.1038/s41580-023-00585-z
[7]	Cheon S Y,Kim H,Rubinsztein D C,et al. Autophagy,cellular aging and age-related human diseases[J]. Exp Neurobiol,2019,28(6):643-657. doi: 10.5607/en.2019.28.6.643
[8]	Lock R,Kenific C M,Leidal A M,et al. Autophagy-dependent production of secreted factors facilitates oncogenic ras-driven invasion[J]. Cancer Discov,2014,4(4):466-479. doi: 10.1158/2159-8290.CD-13-0841
[9]	Tompkins K D,Thorburn A. Regulation of apoptosis by autophagy to enhance cancer therapy[J]. Yale J Biol Med,2019,92(4):707-718.
[10]	Bahrami A,Khazaei M,Hassanian S M,et al. Targeting the tumor microenvironment as a potential therapeutic approach in colorectal cancer: rational and progress[J]. J Cell Physiol,2018,233(4):2928-2936. doi: 10.1002/jcp.26041
[11]	Wang Y,Feng W,Wang X,et al. The multifaceted mechanisms of pristimerin in the treatment of tumors state-of-the-art[J]. Biomed Pharmacother,2022,154:113575. doi: 10.1016/j.biopha.2022.113575
[12]	Shaaban A A,El-Kashef D H,Hamed M F,et al. Protective effect of pristimerin against lps-induced acute lung injury in mice[J]. Int Immunopharmacol,2018,59:31-39. doi: 10.1016/j.intimp.2018.03.033
[13]	Cheng S,Zhang Z,Hu C,et al. Pristimerin suppressed breast cancer progression via mir-542-5p/dub3 axis[J]. Onco Targets Ther,2020,13(1171):6651-6660.
[14]	Li J,Guo Q,Lei X,et al. Pristimerin induces apoptosis and inhibits proliferation,migration in h1299 lung cancer cells[J]. J Cancer,2020,11(21):6348-6355. doi: 10.7150/jca.44431
[15]	Zhao Q,Bi Y,Guo J,et al. Effect of pristimerin on apoptosis through activation of ros/ endoplasmic reticulum (ER) stress-mediated noxa in colorectal cancer[J]. Phytomedicine,2021,80:153399. doi: 10.1016/j.phymed.2020.153399
[16]	Zhang Y,Wang J,Hui B,et al. Pristimerin enhances the effect of cisplatin by inhibiting the miR-23a/Akt/GSK3β signaling pathway and suppressing autophagy in lung cancer cells[J]. Int J Mol Med,2019,43(3):1382-1394.
[17]	Zhao Q,Cheng X,Yu W,et al. Pristimerin induces apoptosis and tumor inhibition of oral squamous cell carcinoma through activating ros-dependent er stress/noxa pathway[J]. Phytomedicine,2021,92(11):153723.
[18]	Zhao Q,Liu Y,Zhong J,et al. Pristimerin induces apoptosis and autophagy via activation of ros/ask1/jnk pathway in human breast cancer in vitro and in vivo[J]. Cell Death Discov,2019,5(87):125.
[19]	石艺,钟燕,刘小虎,等. 扁塑藤素增强顺铂对条件性重编程原代肺癌细胞敏感性的机制[J]. 实用医学杂志,2022,38(7):841-847. doi: 10.3969/j.issn.1006-5725.2022.07.012
[20]	周鑫,杨进,陈林,等. 扁塑藤素对膀胱癌细胞增殖和凋亡的影响及其作用机制[J]. 中国癌症防治杂志,2023,15(1):18-24.
[21]	Condello M,Pellegrini E,Caraglia M,et al. Targeting autophagy to overcome human diseases[J]. Int J Mol Sci,2019,20(3):725. doi: 10.3390/ijms20030725
[22]	Yun C W,Lee S H. The roles of autophagy in cancer[J]. Int J Mol Sci,2018,19(11):725.
[23]	Rakesh R,PriyaDharshini L C,Sakthivel K M,et al. Role and regulation of autophagy in cancer[J]. Biochim Biophys Acta Mol Basis Dis,2022,1868(7):166400. doi: 10.1016/j.bbadis.2022.166400
[24]	Milkovic L,Cipak G A,Cindric M,et al. Short overview of ros as cell function regulators and their implications in therapy concepts[J]. Cells,2019,8(8):793. doi: 10.3390/cells8080793
[25]	Nascimbeni A C,Codogno P,Morel E. Local detection of ptdIns3p at autophagosome biogenesis membrane platforms[J]. Autophagy,2017,13(9):1602-1612. doi: 10.1080/15548627.2017.1341465
[26]	Ko J H,Yoon S O,Lee H J,et al. Rapamycin regulates macrophage activation by inhibiting nlrp3 inflammasome-p38 mapk-nfκb pathways in autophagy- and p62-dependent manners[J]. Oncotarget,2017,8(25):40817-40831. doi: 10.18632/oncotarget.17256

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