Mechanism of Dendrobium Officinale Against Inflammatory Aging Based on Network Pharmacology and Molecular Docking

Ranxi SHI; Tao YANG; Qing CHENG; Liangchen ZHAO; Jiahui GUO; Limei WANG

doi:10.12259/j.issn.2095-610X.S20231101

Volume 44 Issue 11

Nov. 2023

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Ranxi SHI, Tao YANG, Qing CHENG, Liangchen ZHAO, Jiahui GUO, Limei WANG. Mechanism of Dendrobium Officinale Against Inflammatory Aging Based on Network Pharmacology and Molecular Docking[J]. Journal of Kunming Medical University, 2023, 44(11): 1-8. doi: 10.12259/j.issn.2095-610X.S20231101

Citation:

Ranxi SHI, Tao YANG, Qing CHENG, Liangchen ZHAO, Jiahui GUO, Limei WANG. Mechanism of Dendrobium Officinale Against Inflammatory Aging Based on Network Pharmacology and Molecular Docking[J]. Journal of Kunming Medical University, 2023, 44(11): 1-8. doi: 10.12259/j.issn.2095-610X.S20231101

Citation:

Ranxi SHI, Tao YANG, Qing CHENG, Liangchen ZHAO, Jiahui GUO, Limei WANG. Mechanism of Dendrobium Officinale Against Inflammatory Aging Based on Network Pharmacology and Molecular Docking[J]. Journal of Kunming Medical University, 2023, 44(11): 1-8. doi: 10.12259/j.issn.2095-610X.S20231101

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Mechanism of Dendrobium Officinale Against Inflammatory Aging Based on Network Pharmacology and Molecular Docking

doi: 10.12259/j.issn.2095-610X.S20231101

1.
The 2nd Clinical College
2.
The 1st Clinical College
3.
Laboratory Animal Department，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2023-08-18
Available Online: 2023-11-11
Publish Date: 2023-11-30

Abstract

Abstract

Objective To explore the mechanism of Dendrobium officinale against inflammatory aging based on network pharmacology and molecular docking and experimental verification. Methods We used related databases to obtain the action targets of the main components of Dendrobium officinale and the genes of aging rats, then intersected them to obtain the final target. STRING database was used to build a protein-protein interaction（PPI） network. DAVID database was used to conduct enrichment analysis of Gene Ontology（GO） and Kyoto Encyclopedia of Gene and Genomes（KEGG）. AutoDock software was to perform molecular docking verification on the main active components and key targets. Then, the aging rats were gavaged with dendrobium polysaccharide solution for ten week, the expression levels of IL-6 and IL-10 proteins were detected to verify the predicted targets. Results Through database retrieval, 28 active components and 492 target genes were screened out. There were 64 intersections. Enrichment analysis yielded 283 GO functional enrichment entries and 71 KEGG pathway enrichment entries. The main pathway is the PI3K-Akt signaling pathway, Calcium signaling pathway, EGFR tyrosine kinase inhibitor resistance, HIF-1 signaling pathway, Apelin signaling pathway, VEGF signaling pathway, etc. The results of molecular docking showed that each target（AKT1, STAT3, NOS3, GSK3B） and active ingredient（Dentatin, Rutaecarpine, Nodakenetin, Evodaiamine） spontaneously combined and formed stable hydrogen bonds. Animal experiments showed that the content of IL-6 in the medium dose group significantly decreased compared to the blank group, low dose group, and Bailing capsule group, and the content of IL-10 in the low dose group increased compared to the blank group. Conclusions Dendrobium officinale has a delaying effect on the decline of the immune system in elderly rats. It is mainly related to the decrease of IL-6 content and the increase of IL-10 content in the serum. Its mechanism may be related to the expression of key genes AKT1, STAT3, NOS3, GSK3B regulated by components in Dendrobium officinale, such as Dentatin, Rutaecarpine, Nodakenetin, and Evodaiamine.
- Dendrobium officinale,
- Inflammatory aging,
- Network pharmacology,
- IL-6,
- IL-10

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