Artemisinin Mediates the Aerobic Glycolysis of Clear Cell Renal Cell Carcinomas via Regulating ENO2

Yingbao WANG; Xiaoyun LI; Ying TAN; Meng YANG; Yunqiang SHI; Qinrong PING; Libing HU; Yang YANG; Chunhui WANG

doi:10.12259/j.issn.2095-610X.S20231102

Volume 44 Issue 11

Nov. 2023

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Article Navigation > Journal of Kunming Medical University > 2023 > 44(11): 9-15

Yingbao WANG, Xiaoyun LI, Ying TAN, Meng YANG, Yunqiang SHI, Qinrong PING, Libing HU, Yang YANG, Chunhui WANG. Artemisinin Mediates the Aerobic Glycolysis of Clear Cell Renal Cell Carcinomas via Regulating ENO2[J]. Journal of Kunming Medical University, 2023, 44(11): 9-15. doi: 10.12259/j.issn.2095-610X.S20231102

Citation:

Yingbao WANG, Xiaoyun LI, Ying TAN, Meng YANG, Yunqiang SHI, Qinrong PING, Libing HU, Yang YANG, Chunhui WANG. Artemisinin Mediates the Aerobic Glycolysis of Clear Cell Renal Cell Carcinomas via Regulating ENO2[J]. Journal of Kunming Medical University, 2023, 44(11): 9-15. doi: 10.12259/j.issn.2095-610X.S20231102

Citation:

Yingbao WANG, Xiaoyun LI, Ying TAN, Meng YANG, Yunqiang SHI, Qinrong PING, Libing HU, Yang YANG, Chunhui WANG. Artemisinin Mediates the Aerobic Glycolysis of Clear Cell Renal Cell Carcinomas via Regulating ENO2[J]. Journal of Kunming Medical University, 2023, 44(11): 9-15. doi: 10.12259/j.issn.2095-610X.S20231102

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Artemisinin Mediates the Aerobic Glycolysis of Clear Cell Renal Cell Carcinomas via Regulating ENO2

doi: 10.12259/j.issn.2095-610X.S20231102

1.
Dept. of Urology，Yan’an Hospitalof Kunming city，Kunming Yunnan 650051
2.
Dept. of Education，QingDao Eighth People's Hospital，Qingdao Shandong 266041
3.
Dept. of Anesthesiology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2023-09-05
Available Online: 2023-11-04
Publish Date: 2023-11-30

Abstract

Abstract

Objective To investigate Artemisinin-affected cell proliferation and aerobic glycolysis on clear cell renal carcinomas（ccRCC） through regulating ENO2 and clarify the mechanism. Methods ccRCC cell lines OSRC2 and ACHN were exposed to 0, 10, 20, 30, and 40 μmol/L Artemisinin, OSRC2 and ACHN cells treated with 25 μmol/L Artemisinin or transfected with si-ENO2 at the same time. CCK-8 assay detected cell proliferation, glucose consumption, and lactate production were detected by glucose test kit and lactate test kit. The expressions of HK2, LDHA, and ENO2 were detected by Western blot, and ENO2 mRNA expression was detected by RT-qPCR. Results The survival rate of OSRC2 and ACHN cells were decreased with the increase of Artemisinin concentration and treatment time, the IC50 of OSRC2 cells was 25.47 μmol/L, and the IC₅₀ of ACHN was 26.31 μmol/L. ENO2 was upregulated in OSRC2 and ACHN cells（P < 0.01）, and Artemisinin diminished ENO2 expression in cancer cells（all P < 0.05）. Exposure to Artemisinin（25 μmol/L） or knocking down ENO2 significantly inhibited the survival of OSRC2 and ACHN cells（all P < 0.001）, glucose consumption（P < 0.05）, lactate production（all P < 0.05） and protein expression of HK2 and LDHA（all P < 0.05）. Compared with si-ENO2 group, the survival rate（P < 0.001）, glucose consumption（P < 0.05）, lactate production（all P < 0.05） and the expression of HK2 and LDHA（all P < 0.05） of OSRC2 and ACHN cells in knocking down ENO2 and treated with 25 μmol/L Artemisinin group were decreased. Conclusion Artemisinin can inhibit the survival rate and aerobic glycolysis of ccRCC cells, and plays a role by downregulating the expression of ENO2 in ccRCC.
- Clear cells renal cell carcinomas,
- Aerobic glycolysis,
- Artemisinin,
- ENO2,
- Survival rate

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References(24)

References

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